Regulation of Transcription in Nervous Cells

Mandel, P.; Jacob, Monique

doi:10.1007/978-1-4684-8109-9_6

Protein Metabolism of the Nervous System 1970

DOI: 10.1007/978-1-4684-8109-9_6

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Regulation of Transcription in Nervous Cells

P. Mandel¹,

Monique Jacob²

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1973

1978

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Cited by 4 publications

References 54 publications

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The influence of brain cytosol on RNA synthesis and RNA products of isolated mouse brain nuclei

Weck

Johnson

1978

Neurochem Res

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The incubation of isolated nuclei obtained from 10-day-old mouse brain in the presence of brain cell cytosol resulted in an increase in the synthesis of RNA. Under conditions of saturating concentrations of nucleoside triphosphates, the influence of cytosol could not be duplicated by the addition of cyclic nucleotides. The stimulatory activity of cytosol on brain nuclear RNA synthesis could not be attributed to either alterations in the permeability of the nuclear envelope or an increased uptake of radioactively-labeled precursors. Sucrose gradient analysis demonstrated that the RNA products synthesized by nuclei isolated from 10-day-old and adult mouse brain were of a relatively low molecular weight. However, the addition of cytosol resulted in a significant increase in the size of the RNA transcripts. In contrast to the observations with 10-day-old and adult brain nuclei, the RNA from 2-day-old mouse brain nuclei was larger in size and relatively unaffected by the presence of cytosol. Although cytosol caused an increase in the amounts of poly[A]-RNA in nuclei of 2-day-old and adult animals, no comparable effect could be measured in nuclei from 10-day-old brain tissue.

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The influence of brain cytosol on RNA synthesis and RNA products of isolated mouse brain nuclei

Weck

Johnson

1978

Neurochem Res

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Transcriptional and posttranscriptional events associated with neural maturation

Johnson

Weck

1976

Neurochem Res

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tRNA METHYLTRANSFERASE ACTIVITY IN NEONATAL AND MATURE MAMMALIAN NEURAL TISSUE

Johnson

Mathews

Chou

1974

Journal of Neurochemistry

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Abstract— The activity and kinetic characteristics of tRNA methyltransferases were measured with enzyme preparations obtained from neonatal and adult mouse brain tissue. Both neonatal and mature brain enzyme preparations were shown to contain a considerable amount of protein methylase activity which could interfere with the measurement of the tRNA methyltransferases. When increasing amounts of the unfractionated enzymes from young and adult neural tissue were added to reaction mixtures, the saturation kinetics were found to be considerably different. However, fractionation of the samples by precipitation at pH 5 resulted in an increase in the enzyme activity of preparations obtained from adult brain. Although the precipitation at pH 5 allowed a quantitative recovery of the enzyme activity of immature brain samples, this partial purification step led to an apparent activation of the tRNA methyltransferases in adult preparations. This activation was shown to be independent of differential changes in the thermolability of the enzymes but rather to be associated with an increase in the sites methylated and the measured affinity of the adult enzyme preparations with the tRNA substrate. Nicotinamide, a potent inhibitor of tRNA methyltransferase activity in other tissues, was shown to be ineffective in modulating brain tRNA methyltransferase activity. The results are discussed in light of the possible modulation of the activity of specific enzyme species and the alterations in the synthesis of nucleic acid precursors during neural development.

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Regulation of Transcription in Nervous Cells

Cited by 4 publications

References 54 publications

The influence of brain cytosol on RNA synthesis and RNA products of isolated mouse brain nuclei

The influence of brain cytosol on RNA synthesis and RNA products of isolated mouse brain nuclei

Transcriptional and posttranscriptional events associated with neural maturation

tRNA METHYLTRANSFERASE ACTIVITY IN NEONATAL AND MATURE MAMMALIAN NEURAL TISSUE

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