Regulation of vinblastine biosynthesis in cell suspension cultures of catharanthus roseus

Guo, Zhigang; Liu, Yun; Mei-zhen, Gong; Wen, Chen; Li, Wen-Yuan

doi:10.1007/s11240-012-0213-y

Cited by 24 publications

(6 citation statements)

References 34 publications

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“…The biochemicaland metabolic engineering have been studied as a perspective for the creation of new cell lines producing TIAs in large scale bioreactors combined with efficient upstream and downstream processing [15] . Various elicitors of hydroxylase, peroxidase, acetyl transferase and inhibitors of oxygenase on regulation of vinblastine biosynthesis in cell suspension cultures of C. roseus showed Acetyl CoA, Hydrogen peroxide and Benzotriazole to be effective in improving the production of vinblastine [16] .…”

Section: Discussionmentioning

confidence: 99%

Assessment of phytochemical enhancement in Catharanthus roseus by silver nanoparticle

Jan¹,

Naskar²

2021

Pharma Innovation

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Catharanthus roseus (Madagascar periwinkle) is an ornamental plant belonging to the Apocynaceae family which is regarded as a rich source of pharmaceutically important terpenoid indole alkaloids (TIAs). Among these, vinblastine and vincristine are of particular importance because of their wide use in cancer chemotherapy. The present study deals with the preliminary phytochemical screening which reveals the presence of Alkaloids, Flavonoids, Carbohydrates, Saponins, Steroids, Glycosides, Phenols and Tannins in the aqueous extract of the leaf of Catharanthus roseus. The aim of this analysis is to see the significant increase in the amount of production of secondary metabolites in the leaves of Catharanthus roseus. The plant was exposed to silver nanoparticle in the concentration of 0.33 g/kg of soil for three days, resulting in an increase in the amount of secondary metabolites. Qualitative analysis showed the maximum increase of carbohydrate from (83.66 to 261.1 mg/ml) content and Saponins from (8.78 to 40.82 mg/ml) after the application of silver nanoparticles. There was great amplification in alkaloids, phenol and steroid content from (0.006 to 0.187 mg/ml), (0.024 to 0.115mg/ml) and (4.85 mg/ml to 14.88 mg/ml) respectively. Flavanoids content in nanoparticle applied extract of leaves (0.681 mg/ml) showed nearly six times higher concentration than the control plant (0.187 mg/ml).The overall study reveals that the nanoparticles play an important role in augmenting the quantity of secondary metabolites in the leaves and roots of Catharanthus roseus in a substantial manner.

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Section: Discussionmentioning

confidence: 99%

Assessment of phytochemical enhancement in Catharanthus roseus by silver nanoparticle

Jan¹,

Naskar²

2021

Pharma Innovation

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“…Various elicitors of hydroxylase, peroxidase, acetyltransferase and inhibitors of oxygenase were added to a C. roseus cell culture medium to investigate the regulatory effects on tabersonine, vindoline and vinblastine biosynthesis. Hydrogen peroxide was found to be the most effective agent for enhancing the biosynthesis of tabersonine [151].…”

Section: Gmentioning

confidence: 99%

Bioactive Natural Products from Plants and Biotechnological Approaches for their Production

Tripathi

Sapre

Mishra

et al. 2016

Int. J. Biotech. Well. Indus.

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Bioactive natural products are economically important as drugs, fragrances, pigments, food additives and pesticides. The biotechnological tools are important to select, multiply, improve and analyze medicinal plants for production of such products. The utilization of medicinal plant cells for the production of natural or recombinant compounds of commercial interest has gained increasing attention over the past decades. Plant tissue culture systems are possible source of valuable medicinal compounds, fragrances and colorants, which cannot be produced by microbial cells or chemical synthesis. In vitro production of bioactive natural products in plant cell suspension culture has been reported from various medicinal plants and bioreactors are the key step towards commercial production. Genetic transformation is a powerful tool for enhancing the productivity of novel products; especially by Agrobacterium tumefacians. Combinatorial biosynthesis is another approach in the generation of novel natural products and for the production of rare and expensive natural products. Recent advances in the molecular biology, enzymology and bioreactor technology of plant cell culture suggest that these systems may become a viable source of important secondary metabolites. Genetic fingerprinting could be a powerful tool in the field of medicinal plants to be used for correct germplasm identification. In addition, when linked to emerging tools such as metabolomics and proteomics, providing fingerprints of the plant's metabolites or protein composition, it gives data on phenotypic variation, caused by growth conditions or environmental factors, and also yield data on the genes involved in the biosynthesis. DNA profiling techniques like DNA microarrays serve as suitable high throughput tools for the simultaneous analysis of multiple genes and analysis of gene expression that becomes necessary for providing clues about regulatory mechanisms, biochemical pathways and broader cellular functions. New and powerful tools in functional genomics can be used in combination with metabolomics to elucidate biosynthetic pathways of natural products.

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“…Metabolic and biochemical engineering have been studied as a perspective for the creation of new cell lines producing TIAs in large scale bioreactors combined with efficient upstream and downstream processing 64 . Recently, a study to investigate the effect of various elicitors of hydroxylase, peroxidase, acetyltransferase and inhibitors of oxygenase on regulation of vinblastine biosynthesis in cell suspension cultures of C. roseus showed Hydrogen peroxide, Acetyl CoA, Benzotriazole to be very effective in enhancing the production of vinblastine 65 . Table 3 summarises some of the biotechnological approaches used to increase the terpenoid indole alkaloid production.…”

Section: Neuroprotective Activitymentioning

confidence: 99%

Untitled

2013

IJPSR

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Catharanthus roseus is a medicinal herb found in many tropical and subtropical regions around the world. This plant produces a diverse array of secondary metabolites that are pharmaceutically important like vinblastine and vincristine used as chemotherapeutic agents in the treatment of several types of cancers. Low yield of these vinca alkaloids from the plant in vivo and the challenges to meet their high demand worldwide led researchers to develop various in vitro techniques like hairy root culture, callus cultures, shoot cultures, metabolic engineering and regulation studies to increase their production. The present review gives an account of the various phytochemicals derived from the plant and the pharmacological aspects of secondary metabolites studied. Present review also highlights the biotechnological prospects of an efficient and alternative means of production of valuable metabolites from Catharanthus roseus and also from rich microflora residing inside the plant tissues. It gives an emphasis on the need of exploration of diverse niches of endophytes. QUICK RESPONSE CODE

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Regulation of vinblastine biosynthesis in cell suspension cultures of catharanthus roseus

Cited by 24 publications

References 34 publications

Assessment of phytochemical enhancement in Catharanthus roseus by silver nanoparticle

Assessment of phytochemical enhancement in Catharanthus roseus by silver nanoparticle

Bioactive Natural Products from Plants and Biotechnological Approaches for their Production

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