Regulatory ecotoxicity testing of nanomaterials – proposed modifications of OECD test guidelines based on laboratory experience with silver and titanium dioxide nanoparticles

Previous studies have indicated that engineered nanomaterials can be transferred through the food chain. However, their potential ecotoxicity to the environment is not fully understood. Here, we systematically evaluated the physiological behavior and toxicity of polyvinylpyrrolidone (PVP)-coated silver nanoparticles (AgNPs) using a food chain model from Escherichia coli (E. coli) to Caenorhabditis elegans (C. elegans). Our results demonstrated that AgNPs accumulated in E. coli could be transferred to the C. elegans, and AgNPs were clearly distributed in the gut lumen, subcutaneous tissue and gonad. After being transferred to C. elegans through the food chain, the accumulated AgNPs caused serious toxicity to the higher trophic level (C. elegans), including effects on germ cell death, reproductive integrity and life span. Relative to larger particles (75 nm), small AgNPs (25 nm) more easily accumulated in the food chain and exhibited a stronger toxicity to the higher trophic level. More importantly, both the AgNPs that had accumulated in C. elegans through the food chain and the resulting impairment of germ cells could be transferred to the next generation, indicating that AgNP can cause genetic damage across generations. Our findings highlight that nanomaterials pose potential ecotoxicity to ecosystems via transport through the food chain.

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“…Meanwhile, Hund et al . obtained ecotoxicological data for AgNPs with the modified OECD test guidelines12.…”

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confidence: 99%

Insights into the Ecotoxicity of Silver Nanoparticles Transferred from Escherichia coli to Caenorhabditis elegans

Luo

Yang

et al. 2016

Sci Rep

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“…Organic matter is highly important in the control of NM behavior in soils and the OECD soil uses peat that sorbs NMs so strongly compared to other organic material 98 that a reduction of peat quantity has been advised for OECD testing of NMs. 99 Moreover, saturated column experiments cannot be done in the OECD soil because of the high clay content (20 %) 65,100 and using this reference soil has been shown to lead to variability between toxicity assays. 101 Interfaces with other media.…”

Section: Key Challenges and Trade-offs Guiding Parameter And Benchmarmentioning

confidence: 99%

Harmonizing across environmental nanomaterial testing media for increased comparability of nanomaterial datasets

Geitner

Hendren

Cornelis

et al. 2020

Environ. Sci.: Nano

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“…Their applicability to 1 With 'nanomaterials' often defined as materials having one or more dimensions in the size range of 1-100 nm (e.g. EU, 2011). nanomaterial testing can therefore be questioned (Khan et al, 2017;Kühnel & Nickel 2014;Hund-Rinke et al, 2016). The fundamental reason is that nanomaterials are often insoluble or only partly soluble in water.…”

Section: Environmental Hazard and Risk Assessment Of Nanomaterialsmentioning

confidence: 99%

NanoCRED: A transparent framework to assess the regulatory adequacy of ecotoxicity data for nanomaterials – Relevance and reliability revisited

et al. 2017

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2017. NanoCRED: A transparent framework to assess the regulatory adequacy of ecotoxicity data for nanomaterials-Relevance and reliability revisited. NanoImpact, 6 pp.81-89, http://dx. ABSTRACTEnvironmental hazard and risk assessment serve as the basis for regulatory decisions to protect the environment from unintentional adverse effects of chemical substances including nanomaterials. This process requires reliable and relevant environmental hazard data upon which classification and labelling can be based and Predicted No-Effect Concentration (PNEC) values can be estimated. In a regulatory context ecotoxicological data is often recommended to be generated according to accepted and validated test guidelines, preferably also following Good Laboratory Practice. However, engineered nanomaterials are known to behave very differently in ecotoxicity tests compared to the conventional soluble chemicals, for which most guidelines were developed.Therefore non-guideline tests, or tests following modified test guidelines, can provide valuable information and should not per se be considered less adequate for regulatory use. Here we propose a framework for reliability and relevance evaluation of ecotoxicity data for nanomaterials that take into account the challenges and characterisation requirements associated with testing of these substances. The nanoCRED evaluation criteria, and accompanying guidance, were developed to be used in combination with those developed through the 'Criteria for Reporting and Evaluating Ecotoxicity Data (CRED)' project. This approach can accommodate all types of nanomaterials, all types of aquatic ecotoxicity studies, and qualitative as well as quantitative data evaluation requirements. Furthermore, it is practically feasible to implement and directly applicable in European as well as international regulatory frameworks.

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Regulatory ecotoxicity testing of nanomaterials – proposed modifications of OECD test guidelines based on laboratory experience with silver and titanium dioxide nanoparticles

Abstract: -Fordsmand (2016) Regulatory ecotoxicity testing of nanomaterials -proposed modifications of OECD test guidelines based on laboratory experience with silver and titanium dioxide nanoparticles, Nanotoxicology, 10:10, 1442-1447, DOI: 10.1080/17435390.2016

Cited by 106 publications

References 21 publications

Insights into the Ecotoxicity of Silver Nanoparticles Transferred from Escherichia coli to Caenorhabditis elegans

Insights into the Ecotoxicity of Silver Nanoparticles Transferred from Escherichia coli to Caenorhabditis elegans

Harmonizing across environmental nanomaterial testing media for increased comparability of nanomaterial datasets

NanoCRED: A transparent framework to assess the regulatory adequacy of ecotoxicity data for nanomaterials – Relevance and reliability revisited

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