Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium

Abstract

BackgroundUlcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern. Studies have shown that UC is closely associated with the gut microbiota. Garidisan is composed of wild poppy and Artemisia frigida Willd and is commonly used for the treatment of UC in Inner Mongolia, China. In clinical settings, Garidisan has been found to treat UC effectively, with low recurrence. Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear. In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.MethodsThe UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions. High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed. The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.ResultsThe UC mouse model was successfully established and the sequencing results were of adequate quality. There were significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group, and the differences between groups were greater than those within any group. The therapeutic effect of Garidisan on UC is attributed to the direct effect on the Lachnospiraceae family of bacteria.ConclusionGaridisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.

Study of the Influence of Local Strains of Lactobacillus on the Experimental Model of Ulcerative Colitis

“…According to the literature data, an increased level of TNFα is observed in the blood serum and in other pathologies, such as pulmonary dysfunction, abnormalities in the normal course of pregnancy, oncological diseases, bronchial asthma, as well as during the period of exacerbation of diseases of the gastrointestinal tract and during the post-traumatic state, which was also confirmed in our studies [18,24,9,28,29,30].…”

“…For the treatment of active ulcerative colitis, as well as for maintenance therapy, clinical efficacy data is most effective for VSL # 3 and Escherichia coli Nissle 1917. investigated the regulatory effect of garidizan from the wild poppy Artemisia frigida Willd on intestinal microbiota dysbiosis in a mouse model of ulcerative colitis and studied the possible mechanism of the therapeutic effect of garidizan on UC. It has been noted that the therapeutic effect of garidizan on ulcerative colitis is associated with a direct effect on the Lachnospiraceae family of bacteria [18].…”

Preventive effect of Chinese dwarf cherry [Cerasus humilis (Bge.) Sok.] fermentation juice on dextran sulfate sodium-induced ulcerative colitis rats through the regulation of IgA and the intestinal immune barrier

“…Dysbiosis manifests mainly as a decreased diversity of the gut microbiota, a lower quantity of probiotics as the dominant bacteria, and an increase in putrefying bacteria and patho- Food & Function Paper genic bacteria, 26 which is closely related to the occurrence of UC. Oribacterium, Alistipes, and Bacteroidales were significantly enriched in the DSS group.…”

The Influence of Local Strains of Lactobacilli on Experimental Model of Ulcerative Colitis

“…Under physiological conditions the colon epithelial barrier prevents the penetration of bacteria and toxic substances into the internal environment of the body and prevents the development of the inflammatory process. Disturbances of the structure and function of the epithelial barrier can lead to the development of a number of colon diseases, including ulcerative colitis [9].…”

“…Pei LY, Ke YS et al (2019) investigated the regulatory effect of garidisan from wild poppy Artemisia frigidaWilld on microbiota dysbacteriosis intestines in a mouse model of ulcerative colitis and studied the possible mechanism of therapeutic effects of garidisan on UC. It was noted that the therapeutic effect of garidisan on ulcerative colitis is associated with a direct effect on the Lachnospiraceae family of bacteria [9][10].…”