Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology

Peng, Anqi; Yin, Guobin; Zuo, Wenjie; Zhang, Luyao; Du, Guocheng; Chen, Jian; Wang, Yang; Kang, Zhen

doi:10.1016/j.synbio.2024.01.013

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Cited by 3 publications

References 130 publications

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Characterization of a small non-coding RNA S612 in Bacillus subtilis

Peng,

Zhang,

Xiong

et al. 2025

Engineering Microbiology

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Characterization of a small non-coding RNA S612 in Bacillus subtilis

Peng,

Zhang,

Xiong

et al. 2025

Engineering Microbiology

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An autonomous microbial sensor enables long-term detection of TNT explosive in natural soil

Essington,

Vezeau,

Cetnar

et al. 2024

Nat Commun

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Microbes can be engineered to sense target chemicals for environmental and geospatial detection. However, when engineered microbes operate in real-world environments, it remains unclear how competition with natural microbes affect their performance over long time periods. Here, we engineer sensors and memory-storing genetic circuits inside the soil bacterium Bacillus subtilis to sense the TNT explosive and maintain a long-term response, using predictive models to design riboswitch sensors, tune transcription rates, and improve the genetic circuit’s dynamic range. We characterize the autonomous microbial sensor’s ability to detect TNT in a natural soil system, measuring single-cell and population-level behavior over a 28-day period. The autonomous microbial sensor activates its response by 14-fold when exposed to low TNT concentrations and maintains stable activation for over 21 days, exhibiting exponential decay dynamics at the population-level with a half-life of about 5 days. Overall, we show that autonomous microbial sensors can carry out long-term detection of an important chemical in natural soil with competitive growth dynamics serving as additional biocontainment.

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An Autonomous Microbial Sensor Enables Long-term Detection of TNT Explosive in Natural Soil

Essington,

Vezeau,

Cetnar

et al. 2024

Preprint

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Microbes can be engineered to detect target chemicals, but when they operate in real-world environments, it remains unclear how competition with natural microbes affect their performance over long time periods. We engineered sensors and memory-storing genetic circuits insideBacillus subtilisto sense and respond to the TNT explosive, using predictive models for rational design. We characterized their ability to detect TNT in a natural soil system, measuring single-cell and population-level behavior over a 28-day period. The autonomous microbial sensor activated its response by 14-fold when exposed to low TNT concentrations and maintained stable activation for over 21 days, exhibiting exponential decay dynamics at the population-level with a half-life of about 5 days. Our results show that engineered soil bacteria can carry out long-term detection of an important chemical in natural soil with competitive growth dynamics serving as additional biocontainment.

show abstract

Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology

Cited by 3 publications

References 130 publications

Characterization of a small non-coding RNA S612 in Bacillus subtilis

Characterization of a small non-coding RNA S612 in Bacillus subtilis

An autonomous microbial sensor enables long-term detection of TNT explosive in natural soil

An Autonomous Microbial Sensor Enables Long-term Detection of TNT Explosive in Natural Soil

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