Regulatory roles of ganglioside GQ1b in neuronal cell differentiation of mouse embryonic stem cells

Kwak, Dong Hoon; Jin, Jung Woo; Ryu, Ji Won; Ko, Kin Ram; Lee, So Dam; Lee, Jeong-Woong; Kim, Ji Su; Jung, Kyu Yong; Ko, Ki Sung; Yeul, Jin; Hwang, Kyung‐A; Chang, Kyu Tae; Choo, Young‐Kug

doi:10.5483/bmbrep.2011.44.12.799

Cited by 5 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1) (8,9). These gangliosides are thought to be involved in the control of several biological processes, including apoptosis, mouse embryonic development, cell proliferation, cell surface interactions, cell differentiation, and transmembrane signaling (10,11). GM3 is mostly expressed during embryogenesis in mice (12,13), but its expression is decreased during brain development (14).…”

Section: Introductionmentioning

confidence: 99%

Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

Ryu

Chang²,

Lee³

et al. 2012

BMB Reports

Self Cite

View full text Add to dashboard Cite

Gangliosides play important roles in the control of several biological processes, including proliferation and transmembrane signaling. In this study, we demonstrate the effect of ganglioside GM1 on the proliferation of mouse induced pluripotent stem cells (miPSCs). The proliferation rate of miPSCs was lower than in mouse embryonic stem cells (mESCs). Fluorescence activated cell sorting analysis showed that the percentage of cells in the G2/M phase in miPSCs was lower than that in mESCs. GM1 was expressed in mESCs, but not miPSCs. To confirm the role of GM1 in miPSC proliferation, miPSCs were treated with GM1. GM1-treated miPSCs exhibited increased cell proliferation and a larger number of cells in the G2/M phase. Furthermore, phosphorylation of mitogen-activated protein kinases was increased in GM1-treated miPSCs. [BMB Reports 2012; 45(12): 713-718]

show abstract

Section: Introductionmentioning

confidence: 99%

Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

Ryu

Chang²,

Lee³

et al. 2012

BMB Reports

Self Cite

View full text Add to dashboard Cite

show abstract

“…Two 3′ UTR poly(A) sites were identified from ST-MOB for St8sia5 (Table S1), both of which were supported by annotated poly(A) sites from PolyA_DB 3 [45]. St8sia5 has been reported to induce the expression of ganglioside GQ1b and enhance neuronal differentiation via the MAP kinase pathway [46]. Another gene Myef2 (myelin expression factor 2) is a transcriptional repressor of the myelin basic protein gene, which has been found usually upregulated in nerve sheath myxomas and schwannomas [47].…”

Section: Resultsmentioning

confidence: 90%

stAPAminer: Mining Spatial Patterns of Alternative Polyadenylation for Spatially Resolved Transcriptomic Studies

Tang

Sheng

et al. 2022

Preprint

View full text Add to dashboard Cite

Alternative polyadenylation (APA) contributes to transcriptome complexity and gene expression regulation, which has been implicated in various cellular processes and diseases. Single-cell RNA-seq (scRNA-seq) has led to the profile of APA at the single-cell level, however, the spatial information of cells is not preserved in scRNA-seq. Alternatively, spatial transcriptomics (ST) technologies provide opportunities to decipher the spatial context of the transcriptomic landscape within single cells and/or across tissue sections. Pioneering studies on ST have unveiled potential spatially variable genes and/or splice isoforms, however, the pattern of APA usages in spatial contexts remains unappreciated. Here, we developed a toolkit called stAPAminer for mining spatial patterns of APA from spatial barcoded ST data. APA sites were identified and quantified from the ST data. Particularly, an imputation model based on K-nearest neighbors algorithm was designed for recovering APA signals. Then APA genes with spatial patterns of APA usage variation were identified. By analyzing the well-established ST data of mouse olfactory bulb (MOB), we present a detailed view of spatial APA usage across morphological layers of MOB with stAPAminer. We complied a comprehensive list of genes with spatial APA dynamics and obtained several major spatial expression patterns representing spatial APA dynamics in different morphological layers. Extending this analysis to two additional replicates of the MOB ST data, we found that spatial APA patterns of many genes are reproducible among replicates. stAPAminer employs the power of ST for exploring transcriptional atlas of spatial APA patterns with spatial resolution, which is available at https://github.com/BMILAB/stAPAminer.

show abstract

“…The genes RNF165 and EPG5 have been related to neuronal damage with the latter being linked to the multisystem disorder Vici syndrome [Kelly et al, 2013;Zhao et al, 2013]. The involvement of SMAD2 and ST8SIA5 proteins in neural induction and differentiation also makes them ideal candidates for the phenotype of the proband [Chang and Harland, 2007;Kwak et al, 2011]. Further, the LOXHDI gene present in the same locus is associated with nonsyndromic hearing loss [Dror and Avraham, 2010].…”

Section: Discussionmentioning

confidence: 99%

Split Hand/Foot Malformation Associated with 7q21.3 Microdeletion: A Case Report

Sivasankaran¹,

Srikanth²,

Kulshreshtha³

et al. 2015

Mol Syndromol

View full text Add to dashboard Cite

Split hand/foot malformation (SHFM) or ectrodactyly is a rare genetic condition affecting limb development. SHFM shows clinical and genetic heterogeneity. It can present as an isolated form or in combination with additional anomalies affecting the long bones (nonsyndromic form) or other organ systems including the craniofacial, genitourinary and ectodermal structures (syndromic ectrodactyly). This study reports a girl with SHFM who also exhibited developmental delay, mild dysmorphic facial features and sensorineural hearing loss. High-resolution banding analysis indicated an interstitial deletion within the 7q21 band. FISH using locus-specific BAC probes confirmed the microdeletion of 7q21.3. Chromosomal microarray analysis also revealed a microdeletion of 1.856 Mb in 7q21.3. However, a larger 8.44-Mb deletion involving bands 7q21.11q21.2 was observed, and the breakpoints were refined. The phenotype and the candidate genes underlying the pathogenesis of this disorder are discussed.

show abstract

Regulatory roles of ganglioside GQ1b in neuronal cell differentiation of mouse embryonic stem cells

Abstract: Gangliosides play an important role in neuronal differentiation processes. The regulation of ganglioside levels is related to the induction of neuronal cell differentiation. In this study, the

Cited by 5 publications

References 34 publications

Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

stAPAminer: Mining Spatial Patterns of Alternative Polyadenylation for Spatially Resolved Transcriptomic Studies

Split Hand/Foot Malformation Associated with 7q21.3 Microdeletion: A Case Report

Contact Info

Product

Resources

About