Regulatory sequences in the transcription of Neurospora crassa genes: CAAT box, TATA box, Introns, Poly(A) tail formation sequences

Bruchez, J. J. P.; Eberle, Jürgen; Russo, Vincenzo

doi:10.4148/1941-4765.1395

Cited by 83 publications

(60 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TACC repeat is followed by a purine-rich segment. The four introns found in nik-1+ have consensus splice sites that agree with those found in other genes from N. crassa (19).…”

supporting

confidence: 63%

Hyphal development in Neurospora crassa: involvement of a two-component histidine kinase.

Alex

Borkovich

Simon

1996

Proc. Natl. Acad. Sci. U.S.A.

205

View full text Add to dashboard Cite

“…The TACC repeat is followed by a purine-rich segment. The four introns found in nik-1+ have consensus splice sites that agree with those found in other genes from N. crassa (19).…”

supporting

confidence: 63%

Hyphal development in Neurospora crassa: involvement of a two-component histidine kinase.

Alex

Borkovich

Simon

1996

Proc. Natl. Acad. Sci. U.S.A.

205

View full text Add to dashboard Cite

“…We determined the major transcription start sites by RACE experiments (Fig. 1A) and identified a consensus TATA box (8). Northern blot analyses of total RNA extracted from vegetative mycelium revealed a single 1.4-kb hH1 transcript.…”

Section: Resultsmentioning

confidence: 99%

Histone H1 Is Required for Proper Regulation of Pyruvate Decarboxylase Gene Expression in Neurospora crassa

et al. 2003

View full text Add to dashboard Cite

We show that Neurospora crassa has a single histone H1 gene, hH1, which encodes a typical linker histone with highly basic N-and C-terminal tails and a central globular domain. A green fluorescent protein-tagged histone H1 chimeric protein was localized exclusively to nuclei. Mutation of hH1 by repeat-induced point mutation (RIP) did not result in detectable defects in morphology, DNA methylation, mutagen sensitivity, DNA repair, fertility, RIP, chromosome pairing, or chromosome segregation. Nevertheless, hH1 mutants had mycelial elongation rates that were lower than normal on all tested carbon sources. This slow linear growth phenotype, however, was less evident on medium containing ethanol. The pyruvate decarboxylase gene, cfp, was abnormally derepressed in hH1 mutants on ethanol-containing medium. This derepression was also found when an ectopically integrated fusion of the cfp gene promoter to the reporter gene hph was analyzed. Thus, Neurospora histone H1 is required for the proper regulation of cfp, a gene with a key role in the respiratoryfermentative pathway.

show abstract

“…This ORF consists of three exons (229, 673, and 52 bp) divided by two introns (60 and 54 bp) and potentially encodes a 318-amino acid protein. The introns were deduced initially on the basis of consensus sequences for 5 Ј and 3 Ј splice signals typical of fungal genes (Bruchez et al, 1993). To confirm the introns, the cDNA was prepared from total RNA of Mel02010 by reverse transcription-PCR (RT-PCR) with primers B6001 and B6002 (Figure 1).…”

Section: A Putative Open Reading Frame At the Tagged Locusmentioning

confidence: 99%

Plant Colonization by the Vascular Wilt Fungus Fusarium oxysporum Requires FOW1, a Gene Encoding a Mitochondrial Protein

2002

View full text Add to dashboard Cite

The soil-borne fungus Fusarium oxysporum causes vascular wilts of a wide variety of plant species by directly penetrating roots and colonizing the vascular tissue. The pathogenicity mutant B60 of the melon wilt pathogen F. oxysporum f. sp. melonis was isolated previously by restriction enzyme-mediated DNA integration mutagenesis. Molecular analysis of B60 identified the affected gene, designated FOW1 , which encodes a protein with strong similarity to mitochondrial carrier proteins of yeast. Although the FOW1 insertional mutant and gene-targeted mutants showed normal growth and conidiation in culture, they showed markedly reduced virulence as a result of a defect in the ability to colonize the plant tissue. Mitochondrial import of Fow1 was verified using strains expressing the Fow1-green fluorescent protein fusion proteins. The FOW1 -targeted mutants of the tomato wilt pathogen F. oxysporum f. sp. lycopersici also showed reduced virulence. These data strongly suggest that FOW1 encodes a mitochondrial carrier protein that is required specifically for colonization in the plant tissue by F. oxysporum .

show abstract

Regulatory sequences in the transcription of Neurospora crassa genes: CAAT box, TATA box, Introns, Poly(A) tail formation sequences

Cited by 83 publications

References 0 publications

Hyphal development in Neurospora crassa: involvement of a two-component histidine kinase.

Hyphal development in Neurospora crassa: involvement of a two-component histidine kinase.

Histone H1 Is Required for Proper Regulation of Pyruvate Decarboxylase Gene Expression in Neurospora crassa

Plant Colonization by the Vascular Wilt Fungus Fusarium oxysporum Requires FOW1, a Gene Encoding a Mitochondrial Protein

Contact Info

Product

Resources

About