Reinforced Universal Adhesive by Ribose Crosslinker: A Novel Strategy in Adhesive Dentistry

Abstract

Enzymatic biodegradation of demineralized collagen fibrils could lead to the reduction of resin–dentin bond strength. Therefore, methods that provide protection to collagen fibrils appear to be a pragmatic solution to improve bond strength. Thus, the study’s aim was to investigate the effect of ribose (RB) on demineralized resin–dentin specimens in a modified universal adhesive. Dentin specimens were obtained, standardized and then bonded in vitro with a commercial multi-mode adhesive modified with 0, 0.5%, 1%, and 2% RB, restored with resin composite, and tested for micro-tensile bond strength (µTBS) after storage for 24 h in artificial saliva. Scanning electron microscopy (SEM) was performed to analyze resin–dentin interface. Contact angles were analyzed using a contact angle analyzer. Depth of penetration of adhesives and nanoleakage were assessed using micro-Raman spectroscopy and silver tracing. Molecular docking studies were carried out using Schrodinger small-molecule drug discovery suite 2019-4. Matrix metalloproteinases-2 (MMP-2) and cathepsin-K activities in RB-treated specimens were quantified using enzyme-linked immunosorbent assay (ELISA). The significance level was set at α = 0.05 for all statistical analyses. Incorporation of RB at 1% or 2% is of significant potential (p < 0.05) as it can be associated with improved wettability on dentin surfaces (0.5% had the lowest contact angle) as well as appreciable hybrid layer quality, and higher resin penetration. Improvement of the adhesive bond strength was shown when adding RB at 1% concentration to universal adhesive (p < 0.05). Modified adhesive increased the resistance of collagen degradation by inhibiting MMP-2 and cathepsin-K. A higher RB concentration was associated with improved results (p < 0.01). D-ribose showed favorable negative binding to collagen. In conclusion, universal adhesive using 1% or 2% RB helped in maintaining dentin collagen scaffold and proved to be successful in improving wettability, protease inhibition, and stability of demineralized dentin substrates. A more favorable substrate is created which, in turn, leads to a more stable dentin-adhesive bond. This could lead to more advantageous outcomes in a clinical scenario where a stable bond may result in longevity of the dental restoration.

One-Year Clinical Aging of Low Stress Bulk-Fill Flowable Composite in Class II Restorations: A Case Report and Literature Review

“…Thus, the hybrid layer is a mixture of dentin, hydroxyapatite, resin monomers and residual solvents; hence, its stability ultimately depends on the resistance of the individual components to the degradation [51]. In general, the more compact and homogeneous the hybrid layer, the more stable resin-dentin bond is created [34,52] (Figure 2b).…”

Antibacterial and Bonding Properties of Universal Adhesive Dental Polymers Doped with Pyrogallol

“…Despite novel technologies and constant developments in polymer chemistry, there is still a lack of information regarding the bonding performance of modern universal adhesive systems [ 1 ]. Currently, these types of adhesive systems represent an advanced choice for dental practitioners, as they can be used in self-etch, etch-and-rinse, or a selective enamel-etching mode [ 2 , 3 ]. However, the bonding efficiency of universal adhesives is still questioned, as the durability and stability of the dentin–adhesive interface are limited, especially when used in etched dentin [ 4 , 5 , 6 ].…”

Shelf Life and Storage Conditions of Universal Adhesives: A Literature Review

“…Host collagenolytic enzymes (matrix metalloproteinases (MMPs) and cysteine cathepsins), also called ‘dentin degradomics’, play a functional role in resin–dentin bond loss both during and after dentin demineralization. Research in this field is being undertaken to suggest new methods for preventing their activity [ 1 , 2 ].…”

“…Furthermore, Raman microspectroscopy has evolved to become a versatile method for the in situ structural characterization of adhesive-dentin interfaces [ 2 , 37 ]. The Raman spectrum can probe the chemical structure and offer a clear “fingerprint” of the molecules present in a sample.…”

“…Currently, universal adhesive systems are widely utilized due to their simplified application procedures, the less-sensitive techniques needed, and their more user-friendly nature [ 1 ]. Based on this, clinicians are able to use them in either self-etch, etch-and-rinse, and selective enamel-etch modes [ 2 ]. However, the storage of the bonding agent and its shelf-life are considered to be key components in the extraoral degradation phenomena of adhesive systems [ 3 ].…”