Reinforced vesicles withstand rigors of microfluidic electroporation

Robinson, David B.; Lee, Eunice S.; Iqbal, Zohora; Rognlien, Judith L.; Davalos, Rafael V.

doi:10.1016/j.snb.2007.02.028

Cited by 10 publications

(6 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In poly(N-isopropylacrylamide) (NIPAM) gel-filled vesicles of DOPC/DPPC mixture, where the hydrogel was linked physically to the membrane [107,108], micropipette aspiration experiments show that the mechanical stability is largely increased, the lysis tension being 30 times larger than the lysis tension of a pure DOPC membrane. Increase in the tension that can support a gel-filled vesicles membrane has been also reported in phospholipid vesicles filled with poly(allylamine) chains (partially protonated with acetic acid) cross-linked with poly(ethylene glycol) diglycidyl ether [109]. However the authors suggested that The left image (A) is of a HCV at a 38:1 acrylamide to methylenebisacrylamide ratio and the right image (B) is of a HCV twice more reticulated, which presents a lower contact area with the substrate and a lower deformation compared to the unperturbed spherical shape.…”

Section: Gelification Of Internal Cavitymentioning

confidence: 61%

Recent trends in the tuning of polymersomes’ membrane properties

2011

View full text Add to dashboard Cite

Abstract. "Polymersomes" are vesicular structures made from the self-assembly of block copolymers. Such structures present outstanding interest for different applications such as micro-or nano-reactor, drug release or can simply be used as tool for understanding basic biological mechanisms. The use of polymersomes in such applications is strongly related to the way their membrane properties are controlled and tuned either by a precise molecular design of the constituting block or by addition of specific components inside the membrane (formulation approaches). Typical membrane properties of polymersomes obtained from the self-assembly of "coil coil" block copolymer since the end of the nineties will be first briefly reviewed and compared to those of their lipidic analogues, named liposomes. Therefore the different approaches able to modulate their permeability, mechanical properties or ability to release loaded drugs, using macromolecular engineering or formulations, are detailed. To conclude, the most recent advances to modulate the polymersomes' properties and systems that appear very promising especially for biomedical application or for the development of complex and bio-mimetic structures are presented.

show abstract

Section: Gelification Of Internal Cavitymentioning

confidence: 61%

Recent trends in the tuning of polymersomes’ membrane properties

2011

View full text Add to dashboard Cite

show abstract

“…138–146 In their pioneering work, Hang and Rubinsky 138, 139 developed the first microfabricated single-cell electroporation chip containing two facing n+ polysilicon electrodes with two fluidic chambers separated by a silicon nitride dielectric membrane in between (Fig. 2B).…”

Section: The Various Strategies For Microfluidic Electroporationmentioning

confidence: 99%

“…143, 144 This microhole structure was expanded to be applied to electroporation of biomimetic vesicles with similar membrane (phospholipid) and size (10–25 μm) to those of living cells. 145, 146 …”

Section: The Various Strategies For Microfluidic Electroporationmentioning

confidence: 99%

“…Constriction structures such as microscale holes were used for electroporation of single or multiple cells. [138][139][140][141][142][143][144][145][146] In their pioneering work, Hang and Rubinsky 138,139 developed the first microfabricated single-cell electroporation chip containing two facing n+ polysilicon electrodes with two fluidic chambers separated by a silicon nitride dielectric membrane in between (Fig. 2B).…”

Section: Channel Geometry Variation and Constriction Structuresmentioning

confidence: 99%

See 1 more Smart Citation

Microfluidic electroporation for cellular analysis and delivery

2013

View full text Add to dashboard Cite

Electroporation is a simple yet powerful technique for breaching cell membrane barrier. The applications of electroporation can be generally divided into two categories: the release of intracellular proteins, nucleic acids and other metabolites for analysis and the delivery of exogenous reagents such as genes, drugs and nanoparticles with therapeutic purposes or for cellular manipulation. In this review, we go over the basic physics associated with cell electroporation and highlight recent technological advances on microfluidic platforms for conducting electroporation. Within the context of its working mechanism, we summarize the accumulated knowledge on how the parameters of electroporation affect its performance for various tasks. We discuss various strategies and designs for conducting electroporation at microscale and then focus on analysis of intracellular contents and delivery of exogenous agents as two major applications of the technique. Finally, an outlook for future applications of microfluidic electroporation in increasingly diverse utilities is presented.

show abstract

“…Through the use of various microfluidic platforms, monodisperse vesicles between 10 and 40 μm in diameter can be generated with a coefficient of variation as small as 5% (36). The structural integrity of the vesicles can be reinforced to the point of protecting against electroporation by filling the vesicle with poly(allyl ammonium acetate) and cross-linking it into a gel (37). The lipid vesicles can also be immobilized on surfaces to allow simplified monitoring of biochemical processes in a parallel manner (32).…”

Section: Ways To Prevent or Minimize Mass Transportmentioning

confidence: 99%

Controlling Mass Transport in Microfluidic Devices

Kuo

Chiu

2011

Annual Rev. Anal. Chem.

View full text Add to dashboard Cite

Microfluidic platforms offer exquisite capabilities in controlling mass transport for biological studies. In this review, we focus on recent developments in manipulating chemical concentrations at the microscale. Some techniques prevent or accelerate mixing, whereas others shape the concentration gradients of chemical and biological molecules. We also highlight several in vitro biological studies in the areas of organ engineering, cancer, and blood coagulation that have benefited from accurate control of mass transfer.

show abstract

Reinforced vesicles withstand rigors of microfluidic electroporation

Cited by 10 publications

References 38 publications

Recent trends in the tuning of polymersomes’ membrane properties

Recent trends in the tuning of polymersomes’ membrane properties

Microfluidic electroporation for cellular analysis and delivery

Controlling Mass Transport in Microfluidic Devices

Contact Info

Product

Resources

About