Reinsemination by intracytoplasmic sperm injection of 1-day-old oocytes after complete conventional fertilization failure

Morton, Paula C.; Yoder, C.; Tucker, Michael; Wright, Graham; Brockman, Wendy D.W.; Kort, Hilton I.

doi:10.1016/s0015-0282(97)00223-9

Cited by 82 publications

(60 citation statements)

References 10 publications

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“…Conflicting results of rescue ICSI have been reported, depending on the sources of oocytes and timing of rescue ICSI performed. As shown in several previously published reports, although 1-day-old oocytes could be normally fertilised by rescue ICSI (Nagy et al 1995;Park et al 2000), the quality of the ensuing embryos was poor and embryonic developmental potential was severely compromised (Morton et al 1997;Yuzpe et al 2000). However, when the timing of recue ICSI was moved from 22 h to 6 h following insemination, significantly higher rates of fertilisation and improved embryonic development were obtained Nagy et al 2006;Zhu et al 2011), suggesting that rescue ICSI is still effective as long as oocytes have not lost their developmental competence at the time point when it is performed.…”

Section: Discussionmentioning

confidence: 83%

“…Although rescue intra-cytoplasmic sperm injection (ICSI) on 1-day-old oocytes is a backup procedure for complete fertilisation failure or poor fertilisation following conventional IVF, developmental potential of the ensuing embryos is poor due to oocyte ageing (Morton et al 1997;Yuzpe et al 2000;Kuczyński et al 2002). Currently rescue ICSI on 1-day-old oocytes is not recommended for patients with total failure of fertilisation.…”

Section: Introductionmentioning

confidence: 99%

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The value of second polar body detection 4 hours after insemination and early rescue ICSI in preventing complete fertilisation failure in patients with borderline semen

Jin¹,

Shu²,

Dai³

et al. 2014

Reprod. Fertil. Dev.

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Abstract. In this study we evaluated the value of short-time insemination and early rescue intra-cytoplasmic sperm injection (ICSI) in preventing the occurrence of complete fertilisation failure for mild or moderate male infertility patients. A total of 866 couples with borderline semen who underwent in vitro fertilisation treatment in 2010 were included. Regular insemination was performed between January and June of 2010 and short-term insemination was performed from July through December 2010, where, as early as 4 h after insemination, oocytes were denuded from cumulus cells and extrusion of the second polar body was evaluated. Of the 4153 mature oocytes with a detectable second polar body 4 h after insemination, 3874 (93.3%) showed signs of fertilisation on Day 1. Where no second polar body was present in any of the retrieved oocytes for a given patient, rescue ICSI was performed immediately. Similar rates of normal fertilisation and percentage of good-quality embryos were obtained between early rescue ICSI and regular ICSI. Clinical pregnancy occurred in 16 of 43 patients (37.2%) receiving early rescue ICSI. Our results showed early rescue ICSI in combination with evaluation of the second polar body 4 h following insemination is an effective method to prevent complete fertilisation failure for patients with mild or moderate male infertility.Additional keywords: borderline semen, clinical outcome, early rescue ICSI, failed fertilization, second polar body.

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

The value of second polar body detection 4 hours after insemination and early rescue ICSI in preventing complete fertilisation failure in patients with borderline semen

Jin¹,

Shu²,

Dai³

et al. 2014

Reprod. Fertil. Dev.

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show abstract

“…Age showed a significant (p < 0.05) positive correlation with DFI (r ¼ 0.42) and negative correlation with sperm concentration (r ¼ À0.37), vitality (r ¼ À0.37), as well as MSI at both 6 h (r ¼ À0.39) and 24 h (r ¼ À0. 28), and VSI at both 6 h (r ¼ À0.34) and 24 h (r ¼ À0.31). Significant differences were observed in DFI and both MSI and VSI at 6 h and 24 h between patients older than 40 years of age compared to younger patients.…”

Section: Resultsmentioning

confidence: 99%

“…As sperm preparation precedes assisted fertilization, in some cases, such as rescue ICSI, by up to 24 h, sperm survival may be an important determinant of success in many of these procedures [18,28,39]. The use of sperm with a high percentage of DNA damage results in a lower pregnancy rate with IVF and a lower incidence of blastocyst development after ICSI [15,24,27,38].…”

Section: Introductionmentioning

confidence: 99%

Sperm Survival: Relationship to Age-Related Sperm DNA Integrity in Infertile Men

Moskovtsev

Willis

White

et al. 2007

Archives of Andrology

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The purpose of our study was to evaluate the effect of age and sperm DNA integrity on sperm survival. Semen samples from fifty six unselected patients undergoing infertility evaluation were assessed in terms of standard semen parameters, DNA integrity, and sperm survival after 6-24 h of incubation. Prolonged incubation of density gradient selected sperm adversely effects sperm survival in older patients and patients with extensive sperm DNA damage. Immediate preparation of such samples prior to use for assisted reproductive technology (ART) may overcome the negative effect of extended incubation.

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“…Reported fertilization rates using this procedure vary from as low as 28% to as high as 60% (1)(2)(3)5), however; pregnancy rates in general are very low (1-3), and higher 3PN formation has been observed (2,3,6). The timing of rescue ICSI is crucial since as the oocyte ages, the success rate decreases, with decrease in normal fertilization rates and increase in 3PN formation (6).…”

Section: Introductionmentioning

confidence: 99%

Embryonic Chromosomal Abnormalities Obtained After Rescue Intracytoplasmic Sperm Injection of 1-Day-Old Unfertilized Oocytes

Pehlivan

Rubio

Ruı́z

et al. 2004

J Assist Reprod Genet

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Purpose : To study if second day intracytoplasmic sperm injection (ICSI) results in chromosomal abnormalities in the embryos. Methods : Rescue ICSI was performed on 14 metaphase II (MII) oocytes after unsuccessful conventional IVF, four were fertilized. Fluorescent in situ hybridization (FISH) was performed on these four embryos and was informative for three. Results : There were two tetraploid embryos, one mosaic embryo with trisomy 21, tetrasomy 18, and tetrasomy for sex chromosomes in one cell and trisomy 22 in another cell. Conclusions : We discourage the use of second day ICSI due to the observed increase in chromosomal abnormalities in these embryos.

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Reinsemination by intracytoplasmic sperm injection of 1-day-old oocytes after complete conventional fertilization failure

Cited by 82 publications

References 10 publications

The value of second polar body detection 4 hours after insemination and early rescue ICSI in preventing complete fertilisation failure in patients with borderline semen

The value of second polar body detection 4 hours after insemination and early rescue ICSI in preventing complete fertilisation failure in patients with borderline semen

Sperm Survival: Relationship to Age-Related Sperm DNA Integrity in Infertile Men

Embryonic Chromosomal Abnormalities Obtained After Rescue Intracytoplasmic Sperm Injection of 1-Day-Old Unfertilized Oocytes

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