Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration

Wang, Hua; Yang, Yuan; Li, Maofu; Liu, Jiashen; Jin, Wanmei

doi:10.1038/s41598-019-49391-8

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…Then, we found that BA plus 2,4-D depressed shoot regeneration in all treated cultivars but increased callus formation frequencies in some cultivars. Our results are compatible Though explants incubated in the dark can promote shoot regeneration in some plants [17,18,19,20], none of Heuchera's previous in vitro studies addressed the dark treatment effects. The dark treatment favored callus formation but not shoot regeneration in some Heuchera cultivars, according to this study.…”

Section: Discussionsupporting

confidence: 85%

In vitro and cutting propagation of Heuchera: rapid mass production protocol and correlation between initiating ability the two methods

Guo

Wang

et al. 2022

Preprint

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There is still a lack of understanding about the efficient mass propagation of multiple Heuchera cultivars leading to a large market gap. And there has been no empirical research on the relationship between propagation methods of cutting and in vitro. In this study, thirteen different cultivars from the genus Heuchera and Heucherella (Heuchera × Tiarella) were successfully rooted by cutting, and calluses and shoots were also successfully inducted from petiole in vitro culture. However, measured indexes varied significantly among cultivars for cutting and tissue culture. Petiole explants treated with cytokinin 6-benzylaminopurine (BA: 0.5 ~ 2.0 mg/L) plus auxin α-naphthaleneacetic acid (NAA: 0.5 mg/L) formed shoots either directly or indirectly during primary culture. And the dark treatment and 2,4-dichlorophenoxyacetic acid (2,4-D) were ineffective for shoot formation but beneficial for callus stimulation. Furthermore, we discovered a negative correlation between initiating cutting recovery and tissue culture inducting, implying that cultivars with low difficulty in cutting initiating were relatively difficult to tissue culture. Then, we developed a simple and efficient Heuchera rapid propagation protocol. That is, micropropagation through obtaining adventitious shoots directly from primary cultures, supplemented by cutting propagation. Our petiole explants system and leaf-cutting technique are expected to guide Heuchera's mass production.

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Section: Discussionsupporting

confidence: 85%

In vitro and cutting propagation of Heuchera: rapid mass production protocol and correlation between initiating ability the two methods

Guo

Wang

et al. 2022

Preprint

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“…Considering these outcomes, we excluded this PGR combination for shoot regeneration. While dark incubation has been shown to promote shoot regeneration in certain plants [18][19][20][21] , no in vitro studies on Heuchera explored the effects of dark treatment. Our study indicated that dark treatment favored callus formation but did not stimulate shoot regeneration in certain Heuchera cultivars.…”

Section: Discussionmentioning

confidence: 99%

Development and comparative analysis of initiation ability in large-scale Heuchera propagation using tissue culture versus cuttings

Xu,

Guo,

Wang

et al. 2023

Sci Rep

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The Heuchera genus, a member of the Saxifragaceae family, encompasses a wide array of varieties and hybrids, serving both traditional medicinal and ornamental purposes. However, a significant knowledge gap persists in achieving efficient mass propagation of diverse Heuchera cultivars creating a substantial market void. To address this, our study focuses on expedited seedling regeneration by investigating leaf cutting and tissue culture techniques to offer novel insights to cultivators. Herein, we successfully rooted thirteen distinct cultivars from the Heuchera and Heucherella (Heuchera × Tiarella) genera through cutting. Moreover, in vitro culture experiments led to the successful induction of calli and shoots from petiole samples. Notably, variations in measured parameters were observed across cultivars in both cutting and tissue culture methodologies. When petiole explants were exposed to cytokinin 6-benzylaminopurine (BA) at concentrations of 0.5, 1.0, and 2.0 mg/L along with auxin α-naphthaleneacetic acid (NAA) at 0.5 mg/L, shoots were produced either directly or indirectly during the primary culture. Exposure to darkness and the application of 2,4-dichlorophenoxyacetic acid (2,4-D) did not promote shoot formation but were beneficial for callus stimulation. Interestingly, a negative correlation was observed between the ease of initiating cutting recovery and inducting tissue culture regeneration, suggesting that cultivars that easily regenerate through cutting might encounter difficulties during induction by tissue culture. In light of these findings, we devised a streamlined and effective protocol for rapid Heuchera propagation. This protocol involves micropropagation, directly acquiring adventitious shoots from primary cultures supplemented by cutting-based propagation methods.

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“…Similar proceso ha sido observado in vitro en el presente estudio, donde los segmentos basales caulinares sometidos a oscuridad, probablemente permitió la activación de yemas latentes, reflejado en la regeneración de brotes adventicios, lo cual probablemente pueda ser explicado que el tratamiento en oscuridad tiene un efecto positivo en la producción de auxinas que permite la inducción y alargamiento del brote etiolado (Yang et al, 2019;Wang et al, 2019). Por otra parte, Muktadir et al (2016) evaluaron la eficiencia de la regeneración de hipocótilos de plántulas de Solanum melongena, donde los periodos de oscuridad mejoran la regeneración de brotes adventicios, Zhang et al (2016) reportaron que los hipocótilos y meristemos apicales de Ricinus communis requirieron tratamiento de oscuridad para promover un mayor número de brotes adventicios, Wang et al (2019), empleando thidiazuron y 14 días de cultivo bajo oscuridad en explantes foliares de Fragaria vesca obtuvieron alto porcentaje y regeneración de brotes por explante. Makenzi et al (2018), colocando esquejes de Ipomoea batata en oscuridad, demostraron que el número de yemas adventicias fueron mayores en aquellas colocadas bajo luz.…”

Section: Discussionunclassified

Regeneración y enraizamiento de brotes adventicios etiolados de cultivares de zarzamora (Rubus sp)

Millones

Castro

2020

Rev. investig. altoandin.

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The objective of the present study was to evaluate the etiolated adventitious shoot regeneration for the development of an in vitro propagation protocol of three Rubus sp. cultivars. Zygotic embryos were extracted from botanical seeds of Rubus sp. cultivars and placed in establishment culture medium. After 90 days of in vitro culture, the seedlings were conditioned to obtain the basal stem segments and placed in growth and development culture media, they were then subjected to periods of darkness for etiolated adventitious shoot regeneration. Afterwards, the nodal segments were transferred to rooting culture media. Results showed that the regeneration of adventitious shoots from Rubus sp. cultivars was on average eight days. Likewise, the sectioned nodal segments of the adventitious shoots induced roots when the medium contained the combination of the auxins NAA and IBA. The regeneration of etiolated adventitious shoots in the three cultivars of Rubus sp. allowed to obtain elongated shoots in a short time and without the use of growth regulators, facilitating the in vitro propagation of this species.

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Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration

Cited by 6 publications

References 41 publications

In vitro and cutting propagation of Heuchera: rapid mass production protocol and correlation between initiating ability the two methods

In vitro and cutting propagation of Heuchera: rapid mass production protocol and correlation between initiating ability the two methods

Development and comparative analysis of initiation ability in large-scale Heuchera propagation using tissue culture versus cuttings

Regeneración y enraizamiento de brotes adventicios etiolados de cultivares de zarzamora (Rubus sp)

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