Reinwardtia indica: phytochemical screening and evaluation of wound healing activity of the extracts in experimental model rats

Abstract

Reinwardtia indica is traditionally used for wound healing. The main aim of this study was to evaluate the wound healing activity of leaves extracts of R. indica using the excision wound model in rats. The leaves of R. indica were collected from Gondrang, Chitwan, Nepal. Leaves were shade dried, extracted by double maceration and subjected to phytochemical screening. Then, the fusion method was used for the formulation of ointment and evaluated. Rats (n=24) were divided into four groups with 6 in each. Excision wound model was used, 2 cm diameter (314 mm 2 ), 2 mm depth wound was created. The treatment was given daily topically to all groups and the % mean wound contraction rate was calculated on days 4, 8, 12 and 16. The result was analyzed statistically using Graph pad prism version 5. Phytochemical test revealed the presence of alkaloid, flavonoid, tannin, phenol, terpenoid, carbohydrate, etc. All the evaluation parameters showed satisfactory results. The extract of R. indica ointment (2% w/w and 5% w/w) increased the wound contraction rate day by day. The % means wound contraction rate, on day 12, (80% and 88%), and on day 16, (97% and 100%) and statistically significant difference was at p<0.0001. The R. indica extract ointment showed an increased wound contraction rate. So, in further R. indica could be used for commercial production of wound healing ointment.

A Comparative Study on Ascorbic Acid Concentration, Total Phenol, and Flavonoid Content in Citrus Species Grown in a Different Region of Western Nepal

“…Plants are a good source of phytochemicals such as phenol, flavonoid, alkaloids, sterols, terpenoids, phenolic acids, stilbenes, lignans, tannins, and saponins, and some plant also contains vitamin C (ascorbic acid), vitamin E, and carotene [1]. Due to the presence of these phytochemicals, plants show many pharmacological activities such as antioxidant activity, wound healing properties, anti-inflammatory activity, pain healing, antidiarrheal activity, antimicrobial, and anticancer activity [2,3]. Citrus fruits are an important source of different bioactive compounds such as ascorbic acid, flavonoid, and phenol, which have antioxidant activity [4][5][6][7].…”