Related base sequences in the DNA of simple and complex organisms. I. DNA/DNA duplex formation and the incidence of partially related base sequences in DNA

McCarthy, Brian J.; McConaughy, Betty L.

doi:10.1007/bf01458450

Cited by 68 publications

(34 citation statements)

References 54 publications

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“…The experiments of Britten and Kohne (1), Walker (2), McCarthy (3), and others (4,5) have emphasized in recent years that the nuclear DNA from eukaryotic cells contains similar but nonidentical sequences which are repeated many times in the haploid genome. It has been argued that even though some classes of proteins have many members, the frequency of repetition for some DNA sequences exceeds reasonable expectations of the size of families of proteins.…”

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confidence: 99%

An Adenylic Acid-Rich Sequence in Messenger RNA of HeLa Cells and Its Possible Relationship to Reiterated Sites in DNA

Darnell

Wall

Tushinski

1971

Proc. Natl. Acad. Sci. U.S.A.

367

111

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Messenger RNA from HeLa cells contains, as part of the polynucleotide chain, RNase-resistant sequences that are labeled by adenosine but not by uridine. Heterogeneous nuclear RNA also contains adenylate-rich RNase-resistant regions, but in lower proportion than messenger RNA. Hybridization to DNA of 32P-labeled messenger RNA reveals that some of the adenylate-rich region is included in the rapidly-hybridizing fraction.

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confidence: 99%

An Adenylic Acid-Rich Sequence in Messenger RNA of HeLa Cells and Its Possible Relationship to Reiterated Sites in DNA

Darnell

Wall

Tushinski

1971

Proc. Natl. Acad. Sci. U.S.A.

367

111

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“…To test this, we denatured and reassociated aliquots of fractions [11][12][13][14][15][16][17] (Fig. 2) chromatographed on Bio-Gel A-50m.…”

Section: Resultsmentioning

confidence: 99%

Internal organization of long repetitive DNA sequences in sea urchin genomes.

Chaudhari¹,

Craig²

1979

Proc. Natl. Acad. Sci. U.S.A.

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In keeping with earlier reports, we have found that reassociated long repeat DNA from sea urchins is thermostable, indicating the absence of evolutionarily diverged families of repeated sequences. However, we found that when fragnients of radiolabeled long repeat DNA were denatured and reassociated with intact long repeat driver DNA, then sheared to 350 basepairs and assayed for thermal stability, the level of mismatch found in the duplexes varied inversely with the length of the starting fragments. This effect was shown to be due directly to the physical size of the molecules involved in reassociation. These results are consistent with, and support a model for, long repeat DNA in which short units of repetition are arranged in precise arraysp The significance of this arrangement of sequence units within long repeat DNA is discussed.Repeated sequences, composing 20-60% of the nuclear DNA of a large variety of animals, have been shown to fall into two distinct size classes, which are generally referred to as the "long" and "short" repetitive sequences (1). Long and short repeats have been demonstrated within the DNAs of animals in several phyla (2-6). These studies have also revealed that reassociated long repeat sequences are more thermostable than short repeats. Thus, long repeat sequences have been considered to belong to families of identical repeats, whereas short repeat sequences belong to families with evolutionarily diverged copies. Although detailed studies have been performed on the sequence complexity, copy number, and electron microscopic appearance of both long and short repeat DNA (3, 5-7), until recently, little was understood of the internal organization of the majority of long repeat sequences.During the course of our earlier investigations, we discovered that when long repeat DNA was sheared, denatured, and then reassociated with itself, mismatched duplexes were produced (8,9). This indicated that long repeat DNA might in fact contain divergent families of repeats. Therefore, we postulated a model in which (i) long repeat sequences are composed of tandem short units of repetition, 150-300 basepairs (bp) long, (ii) some of these units are members of evolutionarily diverged families of more or less related sequences, and (iii) the units of repetition are precisely ordered, with a certain version of a sequence always adjacent to specific other sequences (8,9). This precise arrangement would favor the formation, in intact arrays, of long and perfectly matched duplexes over short, mismatched ones (see ref. 9).The present paper is aimed at testing some aspects of this model. We have investigated the relationships between fragment length and criterion of reassociation on the one hand and the stability of duplexes formed during the reassociations of long repeat DNA on the other. The results reported here strongly support our model for the presence, and precise tandem ar- Long repeat sequences were prepared from radiolabeled or unlabeled DNA, after single strands, 4000-6000 nucleotides long, had ...

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“…We made DNA filters (12) by denaturing DNA and letting the solution pass through cellulose nitrate membrane filter material to which it bound tightly. (Picture a smooth sheet of paper with little DNA strands bristling out from its surface like whiskers.)…”

Section: Filter-bound Dna Experimentsmentioning

confidence: 99%

My Secret Life

Chilton

2018

Annu. Rev. Plant Biol.

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In my early childhood, my parents gave me to my maternal grandparents for a "visit" that extended over a period of nine years. I seemed to be a fairly ordinary student in primary grades, and had to take a remedial general science class upon entering high school, my first exposure to science. It was the teacher of that class, Mr. Auer, who told me that I had scored amazingly high on a science aptitude test given to all freshmen. The people who administered the testing were convinced that I must have cheated somehow. Mr. Auer suggested that I might want to consider a career in science if I liked it. I loved it, and I did. This autobiographical article recounts my changing interests as I became aware of new fields of science during my education and the start of my career. Out of basic studies of a microbe that causes plant cancer, we developed a method to engineer new and useful genes into crop plants.

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Related base sequences in the DNA of simple and complex organisms. I. DNA/DNA duplex formation and the incidence of partially related base sequences in DNA

Cited by 68 publications

References 54 publications

An Adenylic Acid-Rich Sequence in Messenger RNA of HeLa Cells and Its Possible Relationship to Reiterated Sites in DNA

An Adenylic Acid-Rich Sequence in Messenger RNA of HeLa Cells and Its Possible Relationship to Reiterated Sites in DNA

Internal organization of long repetitive DNA sequences in sea urchin genomes.

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