Relation between Endothelial Nitric Oxide Synthase Genotypes and Oxidative Stress Markers in Larynx Cancer

Abstract

Nitric oxide synthase (eNOS/NOS3) is responsible for the endothelial synthesis of nitric oxide (NO•). G894T polymorphism leads to the amino acid substitution from Glu298Asp that causes lower NOS3 activity and basal NO• production in NOS3 894T (298Asp) allele carriers compared with the GG homozygotes. NO• acts as an antioxidant protecting against Fenton's reaction which generates highly reactive hydroxyl radicals. Allelic variation of NOS3 may influence an individual's risk of laryngeal cancer (LC). In the current study we have examined the possible relationship between NOS3 G894T genotypes and various systemic oxidative damage markers such as protein carbonyl, advanced oxidation protein products, Cu, Zn-superoxide dismutase, thiol group fractions, and lipid hydroperoxides in LC patients. Genotyping was carried out by PCR-RFLP. In LC patients with TT genotype, Cu, Zn-superoxide dismutase activities and nonprotein thiol levels were significantly higher than the controls. In patients with GT and GG genotype, high levels of lipid hydroperoxides showed statistical significance when compared to controls. Our results indicate a potential relationship among G894T polymorphism of NOS3, and impaired redox homeostasis. Further studies are required to determine the role of NOS3 gene polymorphism and impaired plasma redox homeostasis.

Renalase as a Novel Biomarker for Evaluating the Severity of Hepatic Ischemia‐Reperfusion Injury

“…As previously described [29, 30], total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. 2  μ g of total RNA was reversely transcripted using an RNA PCR Kit (Takara Biotechnology, Otsu, Japan), and the resulting cDNA was used as a PCR template.…”

Endothelial nitric oxide synthase gene glu298asp polymorphism in preterm neonates with respiratory distress syndrome

“…15 It protects against Fenton's reaction which generates highly reactive hydroxyl (OH•) free radicals. 16 T allele is functionally relevant affecting eNOS expression and enzyme activity decreasing NO, leading to oxidant/antioxidant imbalance with limited ability to reduce levels of iron-generated (FeNO) and non-irongenerated oxidative stress. 17,18 NO is also essential for decreased pulmonary vascular resistance after birth.…”

The Endothelial Nitric Oxide Synthase Gene Variants as a Risk Factor for Chronic Lymphocytic Leukemia

“…25 Almost 400 eNOS variants have been described up to now and it was found that some of them to be related to carcinogenesis. 5 It was reported that some of these variants are significantly related with the development of certain cancer types. 26,27 This case-control study examined the G894T and intron 4 a/b variants of eNOS gene and their relationship to suspectibility for CLL in a Turkish cohort.…”

“…Although, there were studies that the eNOS G894T variant was associated with prostate cancer, bladder cancer, colorectal cancer, larynx cancer, there were studies that the eNOS G894T variant was not associated with cancer and survival. 4,5,7,[39][40][41][42] Lee et al declared that there was no significant associa-International Journal of Hematology and Oncology tion between the eNOS G894T variant and breast cancer, but this variant was associated with lymph node involvement. 43 In present study, we found a significant difference in genotype distribution between patients and controls.…”

“…Nitric oxide synthase (NOS) is the major source of the NO production; convertion of L-arginine to L-citrulline take place during NO production. 5 There are three different isoforms of NOS family. They include neuronal-NOS (nNOS/NOS1), inducible-NOS (iNOS/NOS2), and endothelial-NOS (eNOS/NOS3).…”