Relation of 2-O-methyl-l-fucose to blood group H(0) specificity inTaxus Cuspidata twigs

Isolation of Anticoagulant Fractions from Crude Fucoidin

“…It is remarkable that the most active material contained after hydrolysis only 2 demonstrable sugars, namely fucose and a faster component located between fucose and 2-O-methylfucose on paper chromatograms (2). The experiments reported here show that purified fucoidin fractions from Fucus vesiculosus are potent inhibitors of blood coagulation in vitro and in vivo.…”

“…Paper chromatographic technics employed were those described previously ( 2 ) . Reducing sugar was determined by the method of Folin and Malmros ( 10).…”

Limitations of Heterologous Reagents in the Elucidation of Blood Group H(0) Specific Structures

“…Although free 2-0-methyl-L-fucose is as good an inhibitor of Lotus a- p ere « P agglutinin as it is of eel serum, Taxus polysaccharide, in which 2-0-methyl-L-fucose is believed to be the component responsible for H(0) activity in the eel serum system [22], does not neutralize the Lotus agglutinins. This points to a structural arrangement of 2-0-methyl-L-fucose on the macromolecule which makes it accessible only to the eel reagent.…”

“…These were human ovarian cyst H(O) mucoid [1,21], higher plant polysaccharides from Taxus cuspidata [22] and Sassafras albidum [28] and a lipopolysaccharide from E. coli [10,24] prepared in this laboratory. Monosaccharides and glycosides: Many of these were donated by others [20] and some synthesized in this laboratory [4,20].…”

Observations on a Haemagglutinin from the Freshwater Mussel Anodonta anatina

“…Pooled extracts and extracts of single seeds showed the same activity. Springer et al [20] attributed the blood group H activity of T. cus pidata to 2-0-methyl-L-fucose. Since Chessin and McGinniss [1] found that in reality different eel sera may contain different aggluti nins of varying H"0"I specificities, it was thought that T. baccata seed extract might prove to be of value in determining the specificity of A. anatina extract.…”