Relation of Iron, TIBC and Oxidative Stress with Glycosylated Haemoglobin in Diabetes Mellitus

Abstract

Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycaemia with disturbances of carbohydrate, fat and protein metabolism. Scientific evidences suggest that high iron storage may play a role in pathogenesis of type 2 diabetes. Excess iron accumulation induces organ damage due to the overproduction of ROS through Fenton reaction. Thus, the aim of this study was to find out the relation between serum iron, total iron binding capacity (TIBC) and oxidative stress (OS) with glycosylated haemoglobin (HbA1c) in type 2 diabetes mellitus patients.
 The study consisted of 90 subjects, which were divided into 3 different groups; Group 1 compromised of 30 healthy individuals, Group 2 included 30 T2DM patients with normal glycemic control and Group 3 included 30 T2DM patients with poor glycemic control. Blood samples were collected from the three groups and fasting plasma glucose (FPG), post-prandial plasma glucose (PPPG), HbA1c, Iron, TIBC, Hemoglobin (HB), Malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) levels were analysed.
 We found, that mean levels of FPG, PPPG, HbA1c, Iron and MDA were significantly higher (p < 0.05) and mean levels of TIBC, SOD and CAT were significantly decreased (p < 0.05) in group 3 as compared to group 2 and group 1. There was no significant difference (p > 0.05) observed in iron, TIBC and Hb levels between group 1 and group 2. We found a significant positive correlation of Iron and MDA with HbA1c and significant negative correlation of TIBC, SOD and CAT with HbA1c in group 3.
 In our study we found significant positive correlation of HbA1c with MDA and iron that indicates poor glycemic control leads to increased glycation of haemoglobin and other heme containing proteins. It causes more release of iron that leading to production of oxidative stress and thereby it might plays significant role in early appearance of diabetic complications.