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Introduction: Preterm birth (PTB) is a major determinant of neonatal mortality and morbidity. Preterm babies are prone to serious illness or death during the neonatal period. PTB is one of the unresolved problems in clinical obstetrics and one of the greatest threats to the developing fetus, there is need to determine predictive biomarker for preterm delivery. Therefore present study aimed to assess serum levels of ceruloplasmin and Alkaline phosphatase in preterm and full-term delivery. Materials and Methods: The present study includes total 80 subjects that comprise forty women presenting with preterm onset of labor followed by delivery and forty women who delivered at term served as controls. Blood Samples from the subjects were obtained for ceruloplasmin and Alkaline phosphatase estimation, when patient was in labor. Serum ceruloplasmin and alkaline phosphatase measured spectrophotometrically. Serum ceruloplasmin was estimated by Herbert A Ravin and Henry et al. method. Serum alkaline phosphatase was estimated by Kinetic p-NPP method. Results: Serum ceruloplasmin levels were significantly increased (P<0.001) in preterm delivery as compared to full term delivery. Alkaline phosphatase levels are significantly increased in preterm delivery (p<0.001) as compared to full term delivery. Conclusion: Our study showed that elevated levels of ceruloplasmin and alkaline phosphatase may be associated with preterm delivery in asymptomatic pregnant women. The elevated ALP may be due to mild chronic subclinical infection which may be responsible for preterm delivery. Ceruloplasmin is acute phase reactant, increased due to an antioxidant defence mechanism against oxidative stress.

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Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycaemia with disturbances of carbohydrate, fat and protein metabolism. Scientific evidences suggest that high iron storage may play a role in pathogenesis of type 2 diabetes. Excess iron accumulation induces organ damage due to the overproduction of ROS through Fenton reaction. Thus, the aim of this study was to find out the relation between serum iron, total iron binding capacity (TIBC) and oxidative stress (OS) with glycosylated haemoglobin (HbA1c) in type 2 diabetes mellitus patients. The study consisted of 90 subjects, which were divided into 3 different groups; Group 1 compromised of 30 healthy individuals, Group 2 included 30 T2DM patients with normal glycemic control and Group 3 included 30 T2DM patients with poor glycemic control. Blood samples were collected from the three groups and fasting plasma glucose (FPG), post-prandial plasma glucose (PPPG), HbA1c, Iron, TIBC, Hemoglobin (HB), Malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) levels were analysed. We found, that mean levels of FPG, PPPG, HbA1c, Iron and MDA were significantly higher (p < 0.05) and mean levels of TIBC, SOD and CAT were significantly decreased (p < 0.05) in group 3 as compared to group 2 and group 1. There was no significant difference (p > 0.05) observed in iron, TIBC and Hb levels between group 1 and group 2. We found a significant positive correlation of Iron and MDA with HbA1c and significant negative correlation of TIBC, SOD and CAT with HbA1c in group 3. In our study we found significant positive correlation of HbA1c with MDA and iron that indicates poor glycemic control leads to increased glycation of haemoglobin and other heme containing proteins. It causes more release of iron that leading to production of oxidative stress and thereby it might plays significant role in early appearance of diabetic complications.