Relation of Paraoxonase1, Arylesterase and Lipid Profile in Ischemic Stroke Patients

Chawhan, Seema S; Mogarekar, Mukund Ramchandra; Wagh, Reena V.; Das, Rajkumar R; Pramanik, Sanjay S; Sonune, Sanjay M; Chawhan, Sanjay M

doi:10.7860/jcdr/2015/15345.6707

Cited by 7 publications

(5 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…H19 can cause neuroinflammation by affecting histone deacetylase 1-dependent M1 microglial polarization and may be an important target for the therapy of ischemic stroke (43). On the other hand, acid phosphatase can promote lipid synthesis (44), which causes the risk of ischemic stroke (45). The present study demonstrated that lncRNA-H19 promoting ACP5 protein expression contributed to atherosclerosis and increases the risk of ischemic stroke.…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA-H19 Contributes to Atherosclerosis and Induces Ischemic Stroke via the Upregulation of Acid Phosphatase 5

et al. 2019

View full text Add to dashboard Cite

Objective: Atherosclerosis is closely associated with ischemic stroke, and long noncoding RNA-H19 (lncRNA-H19) might be a potential target for treating atherosclerosis. The present study aimed to investigate the function of lncRNA-H19 in atherosclerosis and to explore a novel therapeutic strategy for ischemic stroke.Methods: Differentially expressed genes (DEGs) in atherosclerosis were screened by searching public database. In combination with the lncRNA-H19-knockout database, potential lncRNA-H19-mediated gene was retrieved and their relationship was identified. In order to assess the detailed regulatory mechanism of lncRNA-H19, we used a lentivirus packaging system to upregulate Acp5 (Acid phosphatase 5) expression in vascular smooth muscle cells (VSMC) and human umbilical vein endothelial cells (HUVECs). The expression of ACP5 was determined by Western Blot, and evaluations of cell proliferation and apoptosis were detected. An ischemic stroke mouse model was established. Atherosclerosis was measured by using plaque area size. The effects H19 on the expression of ACP5 were explored by the overexpression or silence of H19.Results: H19 and ACP5 were associated with Acute Stroke Treatment (TOAST) subtypes of atherosclerotic patients. The target prediction program and dual-luciferase reporter confirmed ACP5 as a direct target of H19. Lentivirus-mediated H19-forced expression upregulated ACP5 protein levels, promoted cell proliferation and suppressed the apoptosis. The plaque area size was larger in ischemic models than controls. The overexpression or silence of H19 increased or reduced the plaque size. The overexpression or silence of H19 resulted in the expression or inhibition of ACP5.Conclusion: IncRNA-H19 promoting ACP5 protein expression contributed to atherosclerosis and increased the risk of ischemic stroke.

show abstract

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA-H19 Contributes to Atherosclerosis and Induces Ischemic Stroke via the Upregulation of Acid Phosphatase 5

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Moreover, during this process, MPO generates the oxidants hypochlorous acid and nitrogen dioxide, which can lead to posttranslational modification of PON1, including tyrosine modifications that inhibit PON1 activity [ 28 ]. In Chawhan et al’s study [ 29 ], PON1 activity was measured spectrophotometrically in 50 ischemic stroke patients and 50 healthy controls and was found to be decreased significantly in patients (p < 0.001). Kotur-Stevuljevic et al investigated oxidative stress status, PON1 status, lipids, and high-sensitivity C-reactive protein (hsCRP) in 185 ACI patients and found decreased HDL-cholesterol level and a remarkable fall in PON1 activity than controls (p < 0.001), along with more prominent inflammation [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Clinicoradiological evaluation of oxidative stress activity in acute cerebral infarction in the first 24 h and the qualitative importance of dysfunctional HDL in stroke

ÜNAL¹,

VURAL²,

EREN³

et al. 2022

Turkish Journal of Medical Sciences

View full text Add to dashboard Cite

Background/aim Acute cerebral infarction (ACI) occurs as a result of instant disruption of vascular flow that causes disbalance between oxidative/antioxidative activity. We examined the relationship of serum neuro-oxidative stress parameters with stroke severity and infarct volume in ACI and emphasized the qualitative importance of high-density lipoprotein (HDL) on its relationship with myeloperoxidase (MPO) and paraoxonase-1 (PON1) in the acute period of stroke. Materials and methods One hundred ACI patients applied within the first 24 h and 50 healthy volunteers were included. The patient group was evaluated with demographic data (including arrival serum biochemical assessment), clinical disability scores, infarct volume, serum oxidative/antioxidative parameters (lipid hydroperoxide (LOOH), MPO, PON1, MPO/PON ratio). The relevant serum parameters were compared with the control group. Dysfunctional HDL measurement was based on detecting dysfunctionality as a result of a high positive correlation between the dysfunctional feature of HDL and the MPO/PON ratio. The correlation of serum parameters, clinical disability score, and infarct volume were evaluated, and independent analyses of variability with comorbidities were performed. Results A negative correlation between PON1 and arrival NIH score/scale (NIHSS), LOOH and discharge modified rankin scale (mRS), triglyceride level, and infarct volume; a positive correlation between MPO\PON ratio and infarct volume was determined. Logistic regression analyses showed that hypertension, diabetes, and high HbA1C may be predictors of stroke severity, and diabetes mellitus, high HbA1C, infarct volume, and high NIHSS score may be predictors of early disability (p < 0.005). The ROC curve analysis revealed that determining the cut-off value for LOOH is of importance in determining early disability scores (7.2 and 6.2, respectively). Conclusion The balance between oxidative and antioxidative stress parameters and their quantitative/qualitative changes is of importance, especially in the acute period of ACI. Dysfunctional HDL’s evolution and its relationship with other oxidants are significant not only in the cardiovascular aspect but also in the clinicoradiological aspect.

show abstract

“…Oxidative stress is suggested to have been caused by free radicals may play role in migraine pathogenesis 18 .…”

Section: Discussionmentioning

confidence: 99%

Aurasız Migrenli Hastalarda Oksidatif Stres Parametreleri

Çakına¹,

Yücel²,

Polat³

et al. 2020

Namık Kemal Tıp Dergisi

View full text Add to dashboard Cite

Migraine is a multifaceted neurological disease whose molecular mechanisms are not yet clearly defined. Oxidative stress is also believed to play a role in the pathogenesis of migraine. The purpose of this study was to evaluate serum paraoxonase (PON) and arylesterase (ARE) activities, thiols levels, ischemia-modified albumin (IMA), the total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) in patients with migraine without aura.Materials and Methods: 30 patients (5 males and 25 females) and 30 matched healthy controls (8 males and 22 females) were enrolled. Serum PON and ARE activities, thiols levels, IMA, TAS, TOS and OSI were determined by using the spectrophotometric method in the study groups. Statistical analysis was conducted using Mann-Whitney U test and independent samples t-test, and p <0.05 was considered as statistically significant.Results: PON and ARE levels were significantly lower in the migraine without aura group compared with the control group (p <0.05). Conclusion:Our results suggested that the PON and ARE levels might be associated with migraine without aura. In migraine patients, it is recommended that oxidative stress markers be investigated with a larger population and these parameters are taken into consideration.

show abstract

Relation of Paraoxonase1, Arylesterase and Lipid Profile in Ischemic Stroke Patients

Cited by 7 publications

References 21 publications

Long Noncoding RNA-H19 Contributes to Atherosclerosis and Induces Ischemic Stroke via the Upregulation of Acid Phosphatase 5

Long Noncoding RNA-H19 Contributes to Atherosclerosis and Induces Ischemic Stroke via the Upregulation of Acid Phosphatase 5

Clinicoradiological evaluation of oxidative stress activity in acute cerebral infarction in the first 24 h and the qualitative importance of dysfunctional HDL in stroke

Aurasız Migrenli Hastalarda Oksidatif Stres Parametreleri

Contact Info

Product

Resources

About