Relation of rice intake and biomarkers of cadmium for general population in Korea

Kim, Soo-Hwaun; Lim, Young-Wook; Park, Kyung-Su; Yang, Ji-Yeon

doi:10.1016/j.jtemb.2017.04.010

Cited by 17 publications

(5 citation statements)

References 14 publications

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“…Studies from other laboratories found that chronic Cd exposure (3 months) resulted in a concentration of 4 mg/L in the blood of the mice (36). This concentration was slightly higher than the general population, but significantly lower than humans with occupational exposures (37)(38)(39)(40)(41). Taken together, these data show that the dose of Cd used in this study is environmentally relevant and comparable to that of human exposures.…”

Section: Micesupporting

confidence: 47%

Prenatal Cadmium Exposure Alters Proliferation in Mouse CD4+ T Cells via LncRNA Snhg7

et al. 2022

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ObjectivePrenatal cadmium (Cd) exposure leads to immunotoxic phenotypes in the offspring affecting coding and non-coding genes. Recent studies have shown that long non-coding RNAs (lncRNAs) are integral to T cell regulation. Here, we investigated the role of long non-coding RNA small nucleolar RNA host gene 7 (lncSnhg7) in T cell proliferation.MethodsRNA sequencing was used to analyze the expression of lncRNAs in splenic CD4+ T cells with and without CD3/CD28 stimulation. Next, T cells isolated from offspring exposed to control or Cd water throughout mating and gestation were analyzed with and without stimulation with anti-CD3/CD28 beads. Quantitative qPCR and western blotting were used to detect RNA and protein levels of specific genes. Overexpression of a miR-34a mimic was achieved using nucleofection. Apoptosis was measured using flow cytometry and luminescence assays. Flow cytometry was also used to measure T cell proliferation in culture. Finally, lncSnhg7 was knocked down in splenic CD4+ T cells with lentivirus to assess its effect on proliferation.ResultsWe identified 23 lncRNAs that were differentially expressed in stimulated versus unstimulated T cells, including lncSnhg7. LncSnhg7 and a downstream protein, GALNT7, are upregulated in T cells from offspring exposed to Cd during gestation. Overexpression of miR-34a, a regulator of lncSnhg7 and GALNT7, suppresses GALNT7 protein levels in primary T cells, but not in a mouse T lymphocyte cell line. The T cells isolated from Cd-exposed offspring exhibit increased proliferation after activation in vitro, but Treg suppression and CD4+ T cell apoptosis are not affected by prenatal Cd exposure. Knockdown on lncSnhg7 inhibits proliferation of CD4+ T cells.ConclusionPrenatal Cd exposure alters the expression of lncRNAs during T cell activation. The induction of lncSnhg7 is enhanced in splenic T cells from Cd offspring resulting in the upregulation of GALNT7 protein and increased proliferation following activation. miR-34a overexpression decreased GALNT7 expression and knockdown of lncSnhg7 inhibited proliferation suggesting that the lncSnhg7/miR-34a/GALNT7 is an important pathway in primary CD4+ T cells. These data highlight the need to understand the consequences of environmental exposures on lncRNA functions in non-cancerous cells as well as the effects in utero.

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Section: Micesupporting

confidence: 47%

Prenatal Cadmium Exposure Alters Proliferation in Mouse CD4+ T Cells via LncRNA Snhg7

et al. 2022

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“…It has been well established that cadmium (Cd) is a toxic environmental pollutant . Diet and smoking are the two major methods by which Cd is taken up by the human body, and long‐term exposure causes chronic Cd toxicity . Cd exhibits toxic effects on various organs and cells in the body .…”

Section: Introductionmentioning

confidence: 99%

Effect of cadmium on osteoclast differentiation during bone injury in female mice

Zhuo

Liu

et al. 2019

Environmental Toxicology

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Cadmium (Cd) is a toxic heavy metal that represents an occupational hazard and environmental pollutant toxic heavy metal, which can cause osteoporosis following accumulation in the body. The purpose of this study was to investigate the effect of Cd on bone tissue osteoclast differentiation in vivo. Female BALB/c mice were randomly divided into three groups and given drinking water with various concentrations of Cd (0, 5, and 25 mg/L) for 16 weeks, after which the mice were sacrificed after collecting urine and blood. The level of Cd, calcium (Ca), phosphorus (P), trace elements, and some biochemical indicators were measured, and the bone was fixed in a 4% formaldehyde solution for histological observation. Bone marrow cells were isolated to determine the expression of osteoclast‐associated mRNA and proteins. Cd was increased in the blood, urine, and bone in response to Cd in drinking water in a dose‐dependent manner. The content of iron (Fe), manganese (Mn), and zinc (Zn) was significantly increased, whereas Ca and P were decreased in bone compared to the control group. Cd affected the histological structure of the bone, and induced the upregulation and downregulation of tartrate‐resistant acid phosphatase 5b (TRACP‐5b) and estradiol in the serum, respectively. Cd had no significant effect on the alkaline phosphatase activity in the serum. The expression of osteoclast marker proteins, including TRACP, cathepsin K, matrix metalloprotein 9, and carbonic anhydrases were all increased in the Cd‐treated bone marrow cells. Cd significantly increased the expression of receptor activator of nuclear factor kappa B ligand (RANKL), but had lower effect on the expression of osteoprotegerin (OPG) in both bone marrow cells and bone tissue. Thus, Cd exposure destroyed the bone microstructure, promoted the formation of osteoclasts in the bone tissue, and accelerated bone resorption, in which the OPG/RANKL pathway may play an important role.

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“…If possible, such studies should be conducted in young individuals who have relatively few factors associated with sleep problems ( 6 ). Further epidemiological investigation is also warranted to explore the sources of cadmium exposure in the Korean population ( 7 ).…”

Section: Dear Editor-in-chiefmentioning

confidence: 99%

High Blood Cadmium Level in Short Sleepers: Results from the 2008–2013 Korean National Health and Nutrition Survey

Yeo¹,

Hwang²,

Ahn³

2021

ijph

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Relation of rice intake and biomarkers of cadmium for general population in Korea

Cited by 17 publications

References 14 publications

Prenatal Cadmium Exposure Alters Proliferation in Mouse CD4+ T Cells via LncRNA Snhg7

Prenatal Cadmium Exposure Alters Proliferation in Mouse CD4+ T Cells via LncRNA Snhg7

Effect of cadmium on osteoclast differentiation during bone injury in female mice

High Blood Cadmium Level in Short Sleepers: Results from the 2008–2013 Korean National Health and Nutrition Survey

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