Oxidative stress is a process where there is an increase in reactive species, both oxygen and nitrogen. This can happen at both systemic and mitochondrial levels. One of the physiological conditions currently associated with oxidative stress is high-performance physical activity. In this way, it is possible to relate that this problem impacts on sports competitions, especially in Jiu-Jitsu, a growing sport in the world and which demands intense physical effort. Thus, the objective was to evaluate the level of mitochondrial and systemic oxidation in leukocytes of jiu-jitsu athletes. 20 adult men were selected for the control group and 13 athletes for the experimental group. A blood collection was performed to perform MTT and chemiluminescence assays for an analysis of mitochondrial and systemic oxidation, respectively. In addition, two reactive nitrogen species were measured: nitric oxide and peroxynitrite. The evaluation of the leukocyte reducing capacity by MTT showed that the athletes presented a greater reducing environment compared to the control. On the other hand, the chemiluminescence evaluation showed that the athletes' leukocytes showed greater systemic oxidation. There was an increase in both reactive nitrogen species in alteta leukocytes. Given this, it was possible to notice that the athletes presented greater systemic oxidative stress, but with mitochondria with better adaptive capacity to the cell's metabolic demands.