Relationship between endogenous hormonal content and somatic organogenesis in callus of peach (Prunus persica L. Batsch) cultivars and Prunus persica×Prunus dulcis rootstocks

Pérez‐Jiménez, Margarita; Cantero-Navarro, Elena; Pérez‐Alfocea, Francisco; Le-Disquet, Isabel; Guivarc’h, Anne; Cos-Terrer, José

doi:10.1016/j.jplph.2014.01.006

Cited by 26 publications

(17 citation statements)

References 34 publications

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“…A correlation between decreased Z levels and embryogenic potential has also been reported. Pérez-Jiménez et al [10] found a higher Z endogenous content in Prunus persica non-embryogenic cultures than in embryogenic ones. Similar results were reported in embryogenic genotypes of hazelnut, where Z levels were found [48], as well as in coconut embryogenic cultures [49].…”

Section: Gsh Supplementation Affects Endogenous Levels Of Aba Z and mentioning

confidence: 97%

“…Podocarpus lambertii Klotzsch ex Endl. (Podocarpaceae) is native from South and Southeastern Brazil and restricted areas of SE is affected by many factors, such as genotype, culture medium composition, plant growth regulators, gelling agent, type of explant, stress, and light [10]. However, among all these factors, plant growth regulators appear to play the most crucial role in this morphogenetic route [11].…”

Section: Introductionmentioning

confidence: 99%

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Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.

et al. 2016

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Here we propose a protocol for embryogenic cultures induction, proliferation and maturation for the Brazilian conifer Podocarpus lambertii, and investigated the effect of abscisic acid (ABA) and glutathione (GSH) supplementation on the maturation phase. ABA, zeatin (Z) and salicylic acid (SA) endogenous levels were quantified. Number of somatic embryos obtained in ABA-supplemented treatment was significant higher than in ABA-free treatment, showing the relevance of ABA supplementation during somatic embryos maturation. Histological analysis showed the stereotyped sequence of developmental stages in conifer somatic embryos, reaching the late torpedo-staged embryo. GSH supplementation in maturation culture medium improved the somatic embryos number and morphological features. GSH 0mM and GSH 0.1mM treatments correlated with a decreased ABA endogenous level during maturation, while GSH 0.5mM treatment showed constant levels. All treatments resulted in decreased Z endogenous levels, supporting the concept that cytokinins are important during the initial cell division but not for the later stages of embryo development. The lowest SA levels found in GSH 0.5mM treatment were coincident with early embryonic development, and this treatment resulted in the highest development of somatic embryos. Thus, a correlation between lower SA levels and improved somatic embryo formation can be hypothesized.

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Section: Gsh Supplementation Affects Endogenous Levels Of Aba Z and mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.

et al. 2016

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“…Cytokinins are categorized as being isoprenoid [N6-2-isopentenyl adenine (IP), trans-zeatin (tZ), cis-zeatin (cZ) and dihydrozeatin (DZ)] or aromatic (N6-benzyladenine, kinetin and topolin) types 16 . Abscisic acid (ABA) and jasmonic acid (JA) are phytohormones involved in the tolerance to abiotic stresses 17 . Growth and differentiation in plant tissue culturing are controlled by interactions among exogenous and endogenous hormones 18,19 .…”

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confidence: 99%

Effects of genotypes and explants on garlic callus production and endogenous hormones

Mostafa

Wang

Song

et al. 2020

Sci Rep

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High callus production is a feasible way to improve the propagation coefficient of garlic. It remains unknown how genotypes and explants affect garlic callus formation. In the present investigation, we found that there were significant differences in callus formation among garlic varieties. Tip explants were the best calli-producing source, and 91.05% of the explants from four varieties, on average, formed calli after 45 d of primary culturing. Upper leaf parts explants produced lower values. Among the different varieties and explant types, tip explants of variety T141 induced calli in the shortest time and had the greatest callus fresh weight at 45 d. An endogenous hormone contents analysis showed that auxins (indole-3-acetic acid and methyl indole-3-acetic acetate), cytokinins (trans-zeatin and dihydrozeatin), gibberellins 4, 9,15,19,24 and 53 , abscisic acid, jasmonic acid, jasmonoyl-L-isoleucine, and dihydrojasmonic acid were significantly greater in the tips than those in the upper leaf parts. High endogenous jasmonic acid content might play important roles in callus formation. These results will help us not only establish an efficient garlic callus induction protocol that can be applied to large-scale callus multiplication and regeneration, and to genetically improvement of garlic production, but also understand endogenous hormone roles in tissue/organ differentiation and dedifferentiation.

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“…Successful in vitro shoot regeneration from seed-derived or leaf explants has been reported for several Prunus species, such as P. persica (Druart, 1999;Gentile et al, 2002;Hammerschlag et al, 1985;Mante et al, 1989;Pooler and Scorza, 1995;Scorza et al, 1990), P. canescens (Antonelli and Druart, 1989), P. padus (Hammatt, 1993), P. domestica Cossio, 1991, 1994;Yancheva, 1993), P. dulcis (Ainsley et al, 2000;Miguel and Oliveira, 1999;Miguel et al, 1996), P. armeniaca (P erez-Tornero et al, 2000), P. serotina (Espinosa et al, 2006;Hammatt and Grant, 1998), P. avium (Bhagwat and Lane, 2004;Canli and Tian, 2008;Feeney et al, 2007;Hammatt and Grant, 1998;Matt and Jehle, 2005;Tang et al, 2002;Zong et al, 2019), P. cerasus (Mante et al, 1989;Sarropoulou et al, 2012;Song and Sink, 2006;Tang et al, 2002), and several hybrid rootstocks (Hasan et al, 2010;Ochatt et al, 1988;P erez-Jim enez et al, 2012P erez-Jim enez et al, , 2014Pooler and Scorza, 1995;San et al, 2015;Sarropoulou et al, 2012;Zhou et al, 2010). For most cultivars of Prunus species, leaf explants are preferable to seedderived explants for maintaining an identical genetic background of the cultivars because of their heterozygosity ...…”

mentioning

confidence: 99%

Adventitious Shoot Regeneration and Agrobacterium tumefaciens-mediated Transient Transformation of Almond × Peach Hybrid Rootstock ‘Hansen 536’

Zong¹,

Denler²,

Danial³

et al. 2019

horts

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Hansen 536' (Prunus dulcis 3 Prunus persica) is an important commercial rootstock for peach and almond. However, susceptibility to wet soil and bacterial canker has limited its use primarily to areas with less annual rainfall. Genetic engineering techniques offer an attractive approach to improve effectively the current problems with this cultivar. To develop an efficient shoot regeneration system from leaf explants, 10 culture media containing Murashige and Skoog (MS) or woody plant medium (WPM) supplemented with different plant growth regulators were evaluated, and adventitious shoot regeneration occurred at frequencies ranging from 0% to 36.1%. Optimal regeneration with a frequency of 32.3% to 36.1% occurred with WPM medium containing 8.88 mM 6-benzylamino-purine (BAP) and 0.98 to 3.94 mM indole-3-butyric acid (IBA). The regenerated shoots had a high rooting ability, and 80% of the in vitro shoots tested rooted and survived after being transplanted to substrate directly. Transient transformation showed an efficient delivery of the b-glucuronidase (GUS) reporter gene (gusA) using all three Agrobacterium tumefaciens strains tested with a concentration of OD 600 0.5 to 1.0 for 4 days of cocultivation. The protocols described provide a foundation for further studies to improve shoot regeneration and stable transformation of the important peach and almond rootstock 'Hansen 536'.

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Relationship between endogenous hormonal content and somatic organogenesis in callus of peach (Prunus persica L. Batsch) cultivars and Prunus persica×Prunus dulcis rootstocks

Cited by 26 publications

References 34 publications

Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.

Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.

Effects of genotypes and explants on garlic callus production and endogenous hormones

Adventitious Shoot Regeneration and Agrobacterium tumefaciens-mediated Transient Transformation of Almond × Peach Hybrid Rootstock ‘Hansen 536’

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