Relationship between gene polymorphisms of two cytokine genes (TNF-α and IL-6) and occurring of lung cancers in the ethnic group Han of China

Abstract

Tumor necrosis factor (TNF) is a cytokine involved in inflammation and TNF-α might be synthesized ectopically in malignant tumors. Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. The present study is to investigate the relationship between genetic polymorphisms of the TNF-α and IL-6 genes and susceptibility to lung cancers in the ethnic group Han of North China. The genotypes in the -238G locus of TNF-α gene and the -572C locus of the IL-6 gene were determined by PCR-RFLP method in 138 patients with lung cancers and 138 healthy individuals. Software PHASE 1.0 was used to analyze the experimental data. The non-conditional logistic regression model was used to analyze the statistical association of genotypes and susceptibility in two groups adjusted by multiple factors. We found that the TNF-α and IL-6 polymorphisms may be a critical risk for the genetic susceptibility to lung cancers in the ethnic group Han of North China. SNP polymorphisms at the -238G locus of TNF-α gene and the -572C locus of the IL-6 gene were detected by the RFLP-PCR method. We found that high rates of single-base G-to-A alteration at the -238G locus of both alleles and high rates of single-base C-to-G alteration at the -572C locus of both alleles correlated with occurring of lung cancers. It is possible that the SNP markers at the -238G locus of TNF-α gene and the -572C locus of the IL-6 gene serve as biological markers of lung cancers upon further study in the future.

RETRACTED ARTICLE: Meta-analysis of the associations between TNF-α or IL-6 gene polymorphisms and susceptibility to lung cancer

“…For example, Liang et al . demonstrated that G-to-A alteration at the -238G locus of the TNF-α gene and C-to-G alteration at the -572C locus of the IL-6 gene correlated with the development of lung cancers [ 24 ]. Chen et al .…”

Evidence for mast cells activation in the lung of propionic acid-induced autism-like rat model (histological and immunohistochemical study)

“…Immunohistochemical result IL-6 revealed few positive reactions in the bronchiolar epithelium and the CT around bronchiole and blood vessel with negative reaction in the interalveolar septa. A similar distribution of IL-6 in the control lung was previously described [58] . On the other side, tissue samples from the PPA treated lung revealed significantly increased in the area percentage of IL-6 in comparison to the control group.…”

Association of TNFα−308, IFNγ+874, and IL10−1082 gene polymorphisms and the risk of non-small cell lung cancer in the population of the South Indian state of Telangana

“…Further, it favors the production of IFN-γ, thereby facilitating the inflammatory process [18]. Single base alterations, namely, G to A and C to G at the -238G to -572C loci, respectively, in the TNF-α gene were found to occur at a very high rate in lung cancer patients [19]. Further, the IL-10 haplotype predicts the survival and relapse of lung cancer, especially NSCLC [20].…”