Relationship between hepatic lipid peroxidation and fibrogenesis in carbon tetrachloride-treated rats: effect of zinc administration

Abstract

1. Lipid peroxidation and hepatic fibrogenesis were investigated in 25 carbon tetrachloride-treated rats and in 25 control animals. Rats were further divided into two groups to receive either a standard diet or one supplemented with zinc. From each group, animals were killed at weeks 3 and 18 of the experiment for histological and biochemical assessments which included hepatic lipid peroxide and collagen concentrations and plasma zinc concentration as well as the hepatic activities of proline hydroxylase and collagenase. 2. Results indicated that oral zinc supplementation was associated with a decrease in lipid peroxidation (mean 51%; P < 0.05), collagen deposition (mean 32%; P < 0.001) and proline hydroxylase activity (mean 30%; P < 0.05) at week 18, together with an increase in collagenase activity (mean 208%; P < 0.01) at week 3, in carbon tetrachloride-treated rats. 3. There was a significant direct correlation between lipid peroxidation and proline hydroxylase activity in carbon tetrachloride-treated rats (r = 0.52; P < 0.01) and also a significant inverse correlation between lipid peroxidation and plasma zinc concentration in these animals (r = -0.62; P < 0.001). 4. These findings are consistent with the hypothesis that hepatic lipid peroxidation plays an important role in the aetiology of hepatic fibrogenesis and that zinc mitigates the process.

A Fermented Substance fromAspergillus phoenicisReduces Liver Fibrosis Induced by Carbon Tetrachloride in Rats

“…33) Furthermore, a close relationship has been reported between lipid peroxidation and fibrogenesis in rats in which fibrosis was induced by CCl 4 administration. 17,34) Our results confirm the finding that hepatic lipid peroxidation is increased during hepatic fibrogenesis. We also found that FSAP inhibited CCl 4 -induced hepatic lipid peroxidation and consequently attenuated the development of liver fibrosis.…”

Adenosine reverses a preestablished CCl4-induced micronodular cirrhosis through enhancing collagenolytic activity and stimulating hepatocyte cell proliferation in rats

“…Enzymes scavenging free oxygen radicals (i.e., glutathione reductase and peroxidase, catalase) are decreased during induction of cirrhosis. 20,50,51 In the present study, oxidative stress lasted long after discontinuation of the hepatotoxin and was evident by the increased presence of carbonyl groups in oxidized proteins (Table 3). Acetaldehyde, the main metabolite of ethanol oxidation, increases both collagen production and procollagen mRNA in levels in vitro, an effect blocked by a scavenger of reducing equivalents.…”

“…13 Total liver collagen was extracted, as previously described in detail, 13 and quantified by its content of hydroxyproline according to the method reported by Rojkind and González. 18 Subcellular fractionation was performed as reported by Aguilar-Delfín et al 19 Whole homogenate was used to measure activity of prolyl hydroxylase (EC 1.1.1.1) by the technique modified by Camps et al, 20 whereas the cytosolic fraction was the source for assessing thymidine kinase (EC 2.7.1.21) activity according to Sauer and Willmanns. 21 In whole homogenate, lipid peroxidation was estimated by the amount of aldehydic products quantified by the thiobarbituric acid reaction (TBARS), and the rate of protein oxidation by the presence of carbonyl groups, respectively.…”

The Effect of Dietary Vitamin E and Zinc Levels on Performance and Lipid Oxidation in Fresh and Stored Eggs of Laying Ducks

“…The addition of zinc in duck diet was effective to be antioxidant that prevent the damage of omega-3 and omega-6 from oxidation process. According to Camps et al (1992), zinc protects fatty acids from peroxidation by inhibiting the production of reactive oxygen species. TBARS value of egg yolk stored at room temperature (29.29 -30.07°C) during 21 days was higher than fresh eggs.…”