Relationship between IgG1 levels and airway responses in guinea pigs actively and passively sensitized to hexahydrophthalic anhydride

Abstract

Organic acid anhydrides (OAAs) are industrial chemicals that may cause induction of specific IgE and airway symptoms in exposed workers. They are a good model for studies of relationships between chemical structure and the sensitizing potential of reactive low-molecular-weight compounds. Hexahydrophthalic anhydride (HHPA) is such a compound. This study aimed to evaluate the relationship between specific IgG1 levels and airway responses in a model to predict the sensitizing potential of OAAs. Guinea pigs were either actively or passively sensitized to HHPA. For active sensitization, guinea pigs were injected i.d. with 0.1 ml of olive oil (vehicle) or 0.05, 0.5, or 5% HHPA in olive oil. Passive sensitization was performed by i.p. injection of different volumes of antisera (0.75-6 ml, either unheated to keep IgE or heated to destroy IgE) taken from HHPA-sensitized guinea pigs. Specific antibody levels were evaluated with ELISA and passive cutaneous anaphylaxis. Animals were challenged 16-18 days after active sensitization, or 2 days after passive sensitization, by intratracheal instillation with HHPA conjugated to guinea pig serum albumin (HHPA-GPSA; 0.05% in saline), and the immediate effects on lung resistance (RL), and plasma extravasation, measured as Evans blue dye extravasation, for up to 6 min were recorded. Active sensitization caused production of specific IgG1. Provocation with HHPA-GPSA caused an increase of both RL and Evans blue dye extravasation, which was dependent upon the active sensitization dose. Challenge with HHPA-GPSA in passively sensitized guinea pigs also produced an increase in both RL and Evans blue dye extravasation which was related to the IgG1 level. In the guinea pig model of HHPA-induced airway allergy, the airway responses are closely related to the serum levels of specific IgG1. Thus, the IgG1 levels induced by the immunization may reflect the sensitizing potential of HHPA.

Asthmalike biphasic airway responses in Brown Norway rats sensitized by dermal exposure to dry trimellitic anhydride powder☆

“…6,7,9,10,12 In addition, a recent study reported nonspecific methacholine airway hyperresponsiveness in Brown Norway rats (sensitized by skin painting with TMA in acetone and olive oil) 24 hours after specific challenge. 11 In the present work, both EAR and LAR were observed in sensitized Brown Norway rats after specific inhalation challenge with TMA.…”

“…Previous studies using animal models demonstrated the production of specific antibodies after exposure by airway inhalation, intradermal injection, or skin painting with OAA (in acetone-oil solvent) and subsequent development of EAR after inhalation challenge to either free OAA or OAA-protein conjugates. [6][7][8][9][10][11] A study of LAR in guinea pigs sensitized to TMA by intradermal injection did not obtain conclusive results, possibly because of the index used to monitor airway responses. 12 To simulate workplace conditions and test the hypothesis that asthmalike responses might occur on the basis of the sensitization to TMA by the dermal route, dry TMA powder was applied topically to the skin.…”

<i>Lactobacillus rhamnosus</i> GG and <i>Lactobacillus casei</i> Suppress <i>Escherichia coli</i>-Induced Chemokine Expression in Intestinal Epithelial Cells

“…After the first report by Strachan [3] in 1989, several environmental factors, including exposure to viral and bacterial microorganisms during early childhood, have been shown to play important roles in the onset of allergic diseases [4,5,6]; these associations are now referred to as the ‘hygiene hypothesis’. On the other hand, intestinal microbiota, an internal environment, might also be involved in the onset of allergic diseases [7]. …”

Antibody responses of rats after immunization with organic acid anhydrides as a model of predictive testing

“…A close cosselation was observed between specific Ig G, titers and lung resistance in guinea pigs sensitized with cis-HHPA (27). The relationship between specific Ig E and the airway response in rats was not evaluated by us in this study.…”