Relationship between in vitro and in vivo methane production measured simultaneously with different dietary starch sources and starch levels in dairy cattle

Hatew, B.; Cone, J.W.; Pellikaan, W.F.; Podesta, S.C.; Bannink, A.; Hendriks, W.H.; Dijkstra, J.

doi:10.1016/j.anifeedsci.2015.01.012

Cited by 34 publications

(29 citation statements)

References 38 publications

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“…microbial composition) may explain differences in the efficacy of additives. Hatew et al (2015) provided further evidence on the importance of diet fed to the donor animal. Experiments involved the incubation of the same substrate (grass silage or beet pulp) with rumen inoculum obtained from donor cows fed on diets that differed in starch source (native vs. gelatinized maize grain) and starch level (270 vs 530 g/kg concentrate DM).…”

Section: Donor Animal Dietmentioning

confidence: 99%

“…More recently, Hatew et al (2015) reported a study comparing measurements of CH 4 production in vitro and in vivo, and those were conducted simultaneously (animals adapted to the same substrate as incubated in bottles were used as a source of rumen inocula for 72 h in vitro incubations). Measurements of CH 4 production for 24 h in vitro (expressed per unit of OM incubated) were found to be moderately correlated (R 2 = 0.54; P = 0.04, 4 diets and 16 animals used) with in vivo CH 4 production (when expressed per unit of estimated rumen-fermentable OM) across a range of diets differing in source and amount of starch in dietary concentrates.…”

Section: In Vitro Versus In Vivomentioning

confidence: 99%

“…Such experimental approaches have allowed the mode of action of a range of chemicals (Busquet et al, 2005;Bodas et al, 2008;García-González et al, 2008;Durmic et al, 2010) and dietary substrates (Patra and Yu, 2013;Hatew et al, 2015) to be investigated. Use of IVFT offers the opportunity to evaluate a broad spectrum of chemical agents alone or in a number of combinations over a wide range of concentrations (e.g., Busquet et al, 2005;García-González et al, 2008).…”

Section: Tablementioning

confidence: 99%

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Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Yáñez-Ruíz

Bannink

Dijkstra

et al. 2016

Animal Feed Science and Technology

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136

120

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a b s t r a c tIn vitro fermentation techniques (IVFT) have been widely used to evaluate the nutritive value of feeds for ruminants and in the last decade to assess the effect of different nutritional strategies on methane (CH4) production. However, many technical factors may influence the results obtained. The present review has been prepared by the 'Global Network' FACCE-JPI international research consortium to provide a critical evaluation of the main factors that need to be considered when designing, conducting and interpreting IVFT experiments that investigate nutritional strategies to mitigate CH 4 emission from ruminants. Given the increasing and wide-scale use of IVFT, there is a need to critically review reports in the literature and establish what criteria are essential to the establishment and implementation of in vitro techniques. Key aspects considered include: i) donor animal species and number of animal used, ii) diet fed to donor animals, iii) collection and processing of rumen fluid as inoculum, iv) choice of substrate and incubation buffer, v) incubation procedures and CH 4 measurements, vi) headspace gas composition and vii) comparability of in vitro and in vivo measurements. Based on an evaluation of experimental evidence, a set of technical recommendations are presented to harmonize IVFT for feed evaluation, assessment of rumen function and CH 4 production.

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Section: Donor Animal Dietmentioning

confidence: 99%

Section: In Vitro Versus In Vivomentioning

confidence: 99%

Section: Tablementioning

confidence: 99%

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Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Yáñez-Ruíz

Bannink

Dijkstra

et al. 2016

Animal Feed Science and Technology

Self Cite

136

120

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“…We did not observe SBO concentration and forage species interaction, and this was probably because of the absence of concentrate in the diets. Besides concentrate diet characteristics, differences between our donor cows' diets and the composition of evaluated forages can lead to alterations in in vitro CH 4 production(Hatew et al, 2015). The same authors observed variable in vitro CH 4 production for different substrates interacting with inoculums from donor cows fed different starch characteristics, and this can be more relevant when evaluating solely forage diets.4.6 | In vitro potentially digestible neutral detergent fibre digestibilityFat supplementation has a known effect of decreasing rumen populations of fibrinolytic bacteria and protozoa and activity of fibredegrading enzymes…”

mentioning

confidence: 99%

Effect of soya bean oil supplementation and forage type on methane production and fibre digestibility using the in vitro gas production system

Rodrigues

Ramin

Huhtanen

et al. 2017

Grass and Forage Science

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We aimed to evaluate the effect of soya bean oil (SBO) supplementation with different forages on in vitro gas production kinetics, methane (CH4) emissions and potentially digestible neutral detergent fibre (pdNDF) digestibility (IVpdNDFD). Samples of whole‐crop maize silage (MS; Zea mays), sugarcane (SC; Saccharum sp.), perennial ryegrass (RG; Lolium perenne), guinea grass (GG; Panicum maximum) and palisadegrass (PG; Brachiaria brizantha) were incubated with three concentrations of SBO (0, 30 and 60 g/kg of dry matter). The interaction between forage species and SBO inclusion affected molar proportion of acetate, acetate‐to‐propionate ratio, asymptotic gas and IVpdNDFD. Acetate‐to‐propionate ratio numerically decreased from 3.56 to 3.44 and 3.77 to 3.56 for MS and SC respectively. Soya bean oil inclusion at 60 g/kg DM decreased 21.2% and 12.9% of IVpdNDFD for MS and SC respectively. Soya bean oil increase did not affect IVpdNDFD for RG, GG and PG. Soya bean oil inclusion decreased quadratically the asymptotic gas (294–265 ml/g OM) for MS and increased linearly (275–283 ml/g OM) for GG. Lower and greater CH4 production was observed for RG and SC respectively. Methane production decreased quadratically by SBO increase. Soya bean oil inclusion linearly decreased CH4 concentration in total gas at 48 hr of incubation from 133 to 128 ml/L. In conclusion, MS and SC are more sensitive to adverse effects of SBO supplementation from 30 to 60 g/kg DM on rumen fermentation when compared to RG, GG and PG.

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“…Assessment of in vitro gas production (GP) is largely used to evaluate the nutritive value of ruminant feeds by incubating substrate in buffered rumen fluid (Cone, Van Gelder, Visscher, & Oudshoorn, 1996;Dijkstra, Kebreab, Bannink, France, & Lopez, 2005;Getachew, Blümmel, Makkar, & Becker, 1998). This in vitro approach can also be used to evaluate different feeding strategies for their potential to mitigate CH 4 production (Hatew et al, 2015;Holtshausen et al, 2012;Pellikaan et al, 2011). There is a lack of studies reporting in vivo CH 4 production by cattle upon changes in the maturity of a grass silage at harvest (Randby, Weisbjerg, Nørgaard, & Heringstad, 2012;Warner, Bannink, Hatew, Van Laar, & Dijkstra, 2017), and there is a dearth of direct in vitro-in vivo comparisons with respect to CH 4 production.…”

Section: Introductionmentioning

confidence: 99%

In vitro gas and methane production in rumen fluid from dairy cows fed grass silages differing in plant maturity, compared to in vivo data

Macome

Pellikaan

Hendriks

et al. 2018

Animal Physiology Nutrition

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The relationship between in vitro rumen CH production of grass silages, using the gas production technique, and in vivo data obtained with the same cows and rations in respiration chambers was investigated. Silages were made from grass harvested in 2013 on May 6th, May 25th, July 1st and July 8th. The grass silages were used to formulate four different rations which were fed to 24 cows in early and late lactation, resulting in a slightly different dry matter intake (DMI; 16.5 kg/day vs. 15.4 kg/day). The experimental rations consisted of 70% grass silage, 10% maize silage, and 20% concentrates on a dry matter basis. Cows were adapted to the rations for 17 days before rumen fluid was collected via oesophageal tubing, and in vitro gas and CH production were analysed. In vitro total gas and CH production of the (ensiled) grass expressed as ml/g OM decreased with advancing maturity of the grass. The in vitro CH production after 48 hr of incubation expressed in ml/g OM did not correlate with the in vivo CH production expressed in g/kg organic matter intake or g/kg DMI (R = .00-.18, p ≥ .287). The differences in CH emission per unit of intake observed in vivo were rather small between the different rations, which also contributed to the observed poor relationship. Utilizing stepwise multiple regression improved the correlation only slightly. In vitro gas and CH production varied based on whether donor cows were previously adapted to the respective ration or not, suggesting that careful adaption to the experimental diet should be envisaged in in vitro gas and CH production experiments.

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Relationship between in vitro and in vivo methane production measured simultaneously with different dietary starch sources and starch levels in dairy cattle

Cited by 34 publications

References 38 publications

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Effect of soya bean oil supplementation and forage type on methane production and fibre digestibility using the in vitro gas production system

In vitro gas and methane production in rumen fluid from dairy cows fed grass silages differing in plant maturity, compared to in vivo data

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