Relationship between increased albendazole systemic exposure and changes in single nucleotide polymorphisms on the β-tubulin isotype 1 encoding gene in Haemonchus contortus

Barrère, Virginie; Álvarez, Luis Ignacio Ortega; Suárez, Gonzalo; Ceballos, Laura; Moreno, Laura; Lanusse, Carlos; Prichard, Roger K.

doi:10.1016/j.vetpar.2011.11.068

Cited by 79 publications

(60 citation statements)

References 21 publications

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“…[27][28][29] The goal in developing such assays was to have reproducible procedures for SNP detection that could be used to monitor for possible resistance selection in MDA programs for human STHs. In our protocol, primer sequences for each reaction were determined and tested against plasmid constructs, each step in the procedure has been tested on field samples of human STHs and the procedures are detailed.…”

Section: Discussionmentioning

confidence: 99%

Molecular and Biological Diagnostic Tests for Monitoring Benzimidazole Resistance in Human Soil-Transmitted Helminths

Diawara¹,

Schwenkenbecher²,

Kaplan³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. In endemic countries with soil-transmitted helminths mass drug administration with albendazole or mebendazole are being implemented as a control strategy. However, it is well known in veterinary helminths that the use of the same benzimidazole drugs can place selection on the β-tubulin gene, leading to resistance. Given the concern that resistance could arise in human soil-transmitted helminths, there is an urgent need to develop accurate diagnostic tools for monitoring resistance. In this study, we developed molecular assays to detect putative resistance genetic changes in Ascaris lumbricoides, Trichuris trichiura, and hookworms, and we optimized an egg hatch assay for the canine hookworm Ancylostoma caninum and applied it to Necator americanus. Both assays were tested on field samples. The molecular assays demonstrated their reproducibility and capacity to detect the presence of worms carrying putative resistance-associated genetic changes. However, further investigations are needed to validate our molecular and biological tests on additional field isolates.

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Section: Discussionmentioning

confidence: 99%

Molecular and Biological Diagnostic Tests for Monitoring Benzimidazole Resistance in Human Soil-Transmitted Helminths

Diawara¹,

Schwenkenbecher²,

Kaplan³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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“…This amount of resistance is due to the concomitance of several factors. The lack of strategic treatment plans and the use of mass-drenching regimes (Arece et al, 2016) are the most important. The use of the same dose for sheep and goats has also likely increased the selection pressure; goats require higher doses than sheep for most anthelmintics (Jackson et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…The prevalence of AR is aggravated by the complexity of the Cuban production systems for small ruminants, where no clear trends toward comprehensive strategies for parasite control have been established and where a single antiparasitic is generally used throughout the year (Delgado-Fernández, 2016). The AS-PCR assay was based on the search for mutations at codon 200 (TTC to TAC) in the gene encoding β-tubulin isotype 1 (Silvestre and Cabaret, 2002;Ghisi et al, 2007;von Samson-Himmelstjerna et al, 2007, Blackhall et al, 2011Barrère et al, 2012). The presence of these mutations in a population causes resistance of the parasites to benzimidazoles.…”

Section: Discussionmentioning

confidence: 99%

First report of multiple anthelmintic resistance in goat farm in Cuba

Arece-García

López-Leyva²,

Olmedo-Juárez

et al. 2017

Helminthologia

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SummaryThis study determined the effi cacies of four classes of anthelmintics (AH) in a goat fl ock where anthelmintic resistance (AR) to ivermectin was suspected. We selected and randomly distributed 105 animals with >500 eggs per gram of feces into seven groups of 15 animals: an untreated control group and groups treated with ivermectin, eprinomectin, albendazole sulfoxide, albendazole, levamisole, and closantel. The goats were individually weighed and treated with the recommended dose. Fecal samples were collected 14 days post-treatment to determine the fecal egg counts and for a fecal egg count reduction test (FECRT). Coprocultures were also performed for identifying any resistant genera. A molecular assay (polymerase chain reaction, PCR) was used to confi rm benzimidazole resistance. The FECRTs for the ivermectin, eprinomectin, albendazole sulfoxide, and albendazole treatments were <90 %, indicating multiple anthelmintic resistance, all in Haemonchus spp. Levamisole had a FECRT confi dence interval <90 %, indicating a moderate level of AR. The PCR detected the ß-tubulin alleles responsible for benzimidazole resistance, confi rming AR. This study is the fi rst monospecifi c report of AR in goats in Cuba, with a total failure of macrocyclic lactones anthelmintic class.

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“…A point mutation in codon 200 (TTC to TAC), causing a phenylalanine to tyrosine substitution, is the most common SNP and often at high frequency in several countries [1,26,27,30] . While the SNPs at codons 167 (TTC to TAC) and 198 (GAA to GCA) are less common and have been reported in a number of different countries [1,11,20,27,31] . In the present study sequence analyses of the isolates which had an over 39.0% resistant allele frequency revealed the existence of only the widest SNP at codon 200 in H. contortus populations in the study area which is in agreement with above studies [1,26,27,30] .…”

Section: Discussionmentioning

confidence: 99%

“…It has been revealed that BZ resistance in trichostrongylid nematodes is particularly associated with the alterations in the β-tubulin isotype 1 gene which has an importance as genetic marker and useful for predicting BZ resistance problems [10] . A point mutations also called as single nucleotide polymorphisms (SNPs) at the codons 167, 198 and 200 of β-tubulin isotype 1 gene have been explored and linked to BZ resistance up to date [11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

Koyunlarda Haemonchus contortus’un Moleküler Prevalansı, Filogenetik Karakterizasyonu ve Benzimidazol Dirençliliği

Önder¹,

Yıldırım²,

İncı³

et al. 2015

Kafkas Univ Vet Fak Derg

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This study was conducted to determine the molecular prevalence and characterization of Haemonchus contortus from sheep along with the benzimidazole (BZ) resistance in H. contortus populations. Fecal samples were collected from a total of 300 sheep in research area and analyzed by fecal flotation. qPCR assays were utilized on trichostrongylid egg positive samples in order to identify H. contortus. Phylogenetic analyses were performed on ribosomal ITS-2 and mt-COI gene regions. BZ sensitive and resistant allele frequencies were determined by qPCR along with sequence analyses of the β-tubulin isotype 1 gene for single nucleotide polimorphizms (SNPs). H. contortus was identified in 36 (24.8%) out of 145 trichostrongylid egg positive samples. H. contortus isolates showed 100.0% identity to each other and 0.1% difference with the isolates available in the GenBank based on phylogenetic analyses of ITS-2 gene while mt-COI analyses of the isolates exhibited a mean of 97.4±0.5% identity to each other and 5.5±0.8% difference with the isolates in GenBank. BZ sensitive and resistant allele frequencies in H. contortus populations were determined as 87.1%±16.2 and 12.9%±16.2, respectively. SNPs were detected only in the codon 200 of the sequenced isolates belong to resistant allele. This study provides the first data on molecular prevalence, phylogenetic characterization and BZ resistance in H. contortus populations from sheep in Turkey. Keywords

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Relationship between increased albendazole systemic exposure and changes in single nucleotide polymorphisms on the β-tubulin isotype 1 encoding gene in Haemonchus contortus

Cited by 79 publications

References 21 publications

Molecular and Biological Diagnostic Tests for Monitoring Benzimidazole Resistance in Human Soil-Transmitted Helminths

Molecular and Biological Diagnostic Tests for Monitoring Benzimidazole Resistance in Human Soil-Transmitted Helminths

First report of multiple anthelmintic resistance in goat farm in Cuba

Koyunlarda Haemonchus contortus’un Moleküler Prevalansı, Filogenetik Karakterizasyonu ve Benzimidazol Dirençliliği

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