Relationship between length variations in Ser/Asp-rich repeats in phosphophoryn and in vitro precipitation of calcium phosphate

Kobuke, Seiji; Suzuki, Shigeki; Harai, Hiroaki; Haruyama, Naoto; Nishimura, Fusanori; Shiba, Hideki

doi:10.1016/j.archoralbio.2015.05.013

Cited by 9 publications

(8 citation statements)

References 30 publications

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“…This indicates that P28 could induce new vessel sprouting via its core domain binding to the BMP‐2 receptor 1A. Lastly, the mechanism of P28 successfully inducing bone regeneration may be mainly as following at least: firstly, P28 binds to BMP‐2 receptor type IA and type II, and induces osteoblastic differentiation via BMP‐2‐smad signal pathway (Javed et al , ); secondly, P28 also contained abundant Asp (aspartic acid) and phosphorylated Ser (serine), which promote and guide mineralization of the natural bone matrix (Kobuke et al , ; Li et al , ); lastly, the controlled release of P28 sustained long‐term biological effect.…”

Section: Discussionmentioning

confidence: 99%

Loading of BMP‐2‐related peptide onto three‐dimensional nano‐hydroxyapatite scaffolds accelerates mineralization in critical‐sized cranial bone defects

Sun

Zhou

Liu

et al. 2017

J Tissue Eng Regen Med

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Extrusion free-forming, as a rapid prototyping technique, is extensively applied in fabricating ceramic material in bone tissue engineering. To improve the osteoinductivity of nano-hydroxyapatite (nHA) scaffold fabricated by extrusion free-forming, in this study, we incorporated a new peptide (P28) and optimized the superficial microstructure after shaping by controlling the sintering temperature. P28, a novel bone morphogenic protein 2 (BMP-2)-related peptide, was designed in this study. Analysis of the structure, physicochemical properties and release kinetics of P28 from nHA sintered at temperatures ranging from 1000 °C to 1400 °C revealed that nHA sintered at 1000 °C had higher porosity, preferable pore size and better capacity to control P28 release than that sintered at other temperatures. Moreover, the nHA scaffold sintered at 1000 °C with P28 showed improved adhesion, proliferation and osteogenic differentiation of MC3T3-E1 cells compared with scaffolds lacking P28 or BMP-2. In vivo, nHA scaffolds sintered at 1000 °C with P28 or BMP-2 induced greater bone regeneration in critical-sized rat cranial defects at 6 and 12 weeks post-implantation compared with scaffolds lacking P28 or BMP-2. Thus, nHA scaffolds sintered at 1000 °C and loaded with P28 may be excellent biomaterials for bone tissue engineering. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

Loading of BMP‐2‐related peptide onto three‐dimensional nano‐hydroxyapatite scaffolds accelerates mineralization in critical‐sized cranial bone defects

Sun

Zhou

Liu

et al. 2017

J Tissue Eng Regen Med

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“…In particular, repetitive amino acids (aspartic acids or glutamic acids) would promote the affinity of P28 to hydroxyapatite (HA) and bone . Phosphorylated serine contributes to nucleation of apatite crystallization and mineralization, an essential step for regeneration of hard bone tissues with proper mechanical properties …”

Section: Introductionmentioning

confidence: 99%

Evaluation of osteogenic inductivity of a novel BMP2‐mimicking peptide P28 and P28‐containing bone composite

Sun

Cui

et al. 2017

J Biomedical Materials Res

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We developed a novel BMP2-mimicking peptide P28, which possesses two distinct special structural elements-seven repetitive aspartic acids and phosphorylated serine designed to promote affinity to hydroxyapatite as well as nucleation of apatite crystallization and mineralization. In this work, we systematically studied the osteogenic inductivity of P28 in vitro and in vivo in comparison with BMP2. We confirmed its effects on proliferation, recruitment, and osteogenic differentiation of MC3T3-E1 cells in vitro. Furthermore, we loaded P28 into a biomimetic bone composite nanohydroxyapatite/collagen/poly(L-lactide) (nHAC/PLA) and evaluated its ability to promote bone regeneration in vivo using a rabbit femoral condyle defect model. The micro-CT reconstruction and quantification analysis and the histological analysis indicated that P28/nHAC/PLA bone composite was capable of repairing bone defects.

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“…Recombinant mouse Dpp deleted 63.5 Ser-Asp repeat regions, accounting for 36.5 % of the length of the Ser-Asp repeat region, were generated and these peptides were able to induce calcium-phosphate precipitation similarly to the full length Dpp at the same concentration. In contrast, the inverted Dpp deleted 63.5 Ser-Asp repeat regions had no effect on the induction of calcium phosphate precipitation (Kobuke et al, 2015). The 8-repeat copy of Asp-Ser-Ser residues facilitates calcium-phosphate precipitation and HA crystal growth, promoting the remineralisation of demineralised human enamel and dentine tubule occlusion (Hsu et al, 2011).…”

Section: Siblings and Dsppmentioning

confidence: 99%

“…To analyse the relationship between length variations in Ser-Asp/Asp-Ser repeat regions and the role of DPP in matrix mineralisation, different lengths of the Ser-Asp/Asp-Ser repeat regions have been generated ( Kobuke et al, 2015 ). Recombinant mouse Dpp deleted 63.5 Ser-Asp repeat regions, accounting for 36.5 % of the length of the Ser-Asp repeat region, were generated and these peptides were able to induce calcium-phosphate precipitation similarly to the full length Dpp at the same concentration.…”

Section: Siblings and Dsppmentioning

confidence: 99%

Dentine sialophosphoprotein signal in dentineogenesis and dentine regeneration

Liu¹,

Li²,

Xia³

et al. 2021

eCM

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Dentineogenesis starts on odontoblasts, which synthesise and secrete non-collagenous proteins (NCPs) and collagen. When dentine is injured, dental pulp progenitors/mesenchymal stem cells (MSCs) can migrate to the injured area, differentiate into odontoblasts and facilitate formation of reactionary dentine. Dental pulp progenitor cell/MSC differentiation is controlled at given niches. Among dental NCPs, dentine sialophosphoprotein (DSPP) is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family, whose members share common biochemical characteristics such as an Arg-Gly-Asp (RGD) motif. DSPP expression is cell- and tissue-specific and highly seen in odontoblasts and dentine. DSPP mutations cause hereditary dentine diseases. DSPP is catalysed into dentine glycoprotein (DGP)/sialoprotein (DSP) and phosphoprotein (DPP) by proteolysis. DSP is further processed towards active molecules. DPP contains an RGD motif and abundant Ser-Asp/Asp-Ser repeat regions. DPP-RGD motif binds to integrin αVβ3 and activates intracellular signalling via mitogen-activated protein kinase (MAPK) and focal adhesion kinase (FAK)-ERK pathways. Unlike other SIBLING proteins, DPP lacks the RGD motif in some species. However, DPP Ser-Asp/Asp-Ser repeat regions bind to calcium-phosphate deposits and promote hydroxyapatite crystal growth and mineralisation via calmodulin-dependent protein kinase II (CaMKII) cascades. DSP lacks the RGD site but contains signal peptides. The tripeptides of the signal domains interact with cargo receptors within the endoplasmic reticulum that facilitate transport of DSPP from the endoplasmic reticulum to the extracellular matrix. Furthermore, the middle- and COOH-terminal regions of DSP bind to cellular membrane receptors, integrin β6 and occludin, inducing cell differentiation. The present review may shed light on DSPP roles during odontogenesis.

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Relationship between length variations in Ser/Asp-rich repeats in phosphophoryn and in vitro precipitation of calcium phosphate

Abstract: The generated rPP-Δ63.5 SDrr which can be substituted for rPP-full may be a candidate for a therapeutic molecule to facilitate hard tissue generation such as reparative dentin formation.

Cited by 9 publications

References 30 publications

Loading of BMP‐2‐related peptide onto three‐dimensional nano‐hydroxyapatite scaffolds accelerates mineralization in critical‐sized cranial bone defects

Loading of BMP‐2‐related peptide onto three‐dimensional nano‐hydroxyapatite scaffolds accelerates mineralization in critical‐sized cranial bone defects

Evaluation of osteogenic inductivity of a novel BMP2‐mimicking peptide P28 and P28‐containing bone composite

Dentine sialophosphoprotein signal in dentineogenesis and dentine regeneration

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