Relationship between &lt;em&gt;IGF2BP2&lt;/em&gt; and &lt;em&gt;IGFBP3&lt;/em&gt; polymorphisms and susceptibility to non-small-cell lung cancer: a case&amp;ndash;control study in Eastern Chinese Han population

Chen, Shu-Chen; Qiu, Hao; Liu, Chao; Wang, Yafeng; Tang, Weifeng; Kang, Mingqiang

doi:10.2147/cmar.s169222

Cited by 18 publications

(19 citation statements)

References 36 publications

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“…The relationship between IGF2BP2 and PI3K/ Akt signaling pathway has been discussed, suggesting up-regulated IGF2BP2 in pancreatic cancer plays a role in cell proliferation [33]. In addition, SNPs of IGF2BP2 and IGF2BP3 has been proved to promote the lymph node metastasis of esophagogastric junction adenocarcinoma [34]. In our GO and KEGG analysis, we noticed that IGF2BP2-related module was correlated with central carbon metabolism, thyroid hormone synthesis and HIF-1signaling pathway, which provided some potential regulatory metabolism of m 6 A RNA modification.…”

Section: Discussionmentioning

confidence: 99%

Identification and validation of m6A RNA methylation regulators with clinical prognostic value in Papillary thyroid cancer

Wang

Zhang

et al. 2020

Cancer Cell Int

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Background: Papillary thyroid cancer (PTC) is a type of malignant tumor with excellent prognosis, accounting for more than 80% of thyroid cancer. Recently, numerous studies illustrated the importance of N 6-methyladenosine (m 6 A) RNA modification to tumorigenesis, but it has never been reported in PTC. Methods: We downloaded data from The Cancer Genome Atlas (TCGA) and analyzed RNA expression, single nucleotide polymorphisms (SNPs) and copy number variations (CNVs) of 19 m 6 A RNA methylation regulators in PTC. Then we used nonnegative matrix factorization (NMF) to cluster patients into two m 6 A subtypes and compared them in overall survival (OS) and disease-free survival (DFS). The Weighted correlation network analysis (WGCNA) and univariate Cox proportional hazard model (CoxPH) were used to select genes for the construction of a m 6 A-related signature. The accuracy and prognostic value of this signature were validated by using receiver operating characteristic (ROC) curves, K-M (Kaplan-Meier) survival analysis, univariant and multivariant analyses. Results: CNVs and differential expression of m 6 A regulators were observed in PTC patients. Especially IGF2BP2 (Insulin-like growth factor 2 mRNA binding protein 2), which was most significantly overexpressed in tumor tissue. We chose 4 genes in the m 6 A-related module from WGCNA: IGF2BP2, STT3A, MTHFD1 and GSTM4, and used them to construct a m 6 A-related signature. The prognostic value of this signature was validated, and risk scores provided by the signature was the independent prognostic factor for PTC. A nomogram was also provided for clinical usage. Conclusions: We performed a comprehensive evaluation of the m 6 A RNA modification landscape of PTC and explored its underlying mechanisms. Our m 6 A-related signature was of great significance in predicting the DFS of patients with PTC. And IGF2BP2 was a gene worthy for further analysis as its strong correlation with DFS and clinical phenotypes of PTC.

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Section: Discussionmentioning

confidence: 99%

Identification and validation of m6A RNA methylation regulators with clinical prognostic value in Papillary thyroid cancer

Wang

Zhang

et al. 2020

Cancer Cell Int

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“…Values represent the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01. lung cancer 33,34 . Previous studies have reported that IGFBP3 expression is increased in many tumors with metastasis, including lung cancer and oral cancer 35,36 .…”

Section: Discussionmentioning

confidence: 99%

“…IGFBP3 also exhibits IGF-independent effects. Previous studies have shown that IGFBP3 expression is correlated with various cancers, such as esophageal cancer 24 , 25 , gastric cancer 26 , colorectal cancer 27 , 28 , pancreatic cancer 29 , prostate cancer 30 – 32 , and lung cancer 33 , 34 . Previous studies have reported that IGFBP3 expression is increased in many tumors with metastasis, including lung cancer and oral cancer 35 , 36 .…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0046263 functions as a ceRNA to promote nasopharyngeal carcinoma progression by upregulating IGFBP3

Chen

et al. 2020

Cell Death Dis

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Accumulating evidences indicate that circular RNAs (circRNAs), a subclass of noncoding RNAs, play important role in regulating gene expression in eukaryotes. Hsa_circ_0046263 (circ-0046263) was found aberrantly expressed in nasopharyngeal carcinoma (NPC), but its role in tumor growth and metastasis remains largely unclear. Sanger sequencing, RNase R assay, and nucleic acid electrophoresis were conducted to verify the identification of circ-0046263. Nuclear separation and fluorescence in situ hybridization (FISH) assays were used to determine the localization of circ-004263. Dual luciferase reporter and RNA immunoprecipitation (RIP) were employed to confirm the binding of circ-0046263 with miR-133a-5p. Colony formation, proliferation, wound healing, transwell, western blot, and in vivo tumor growth and metastasis assays were performed to assess the roles of circ-0046263, miR-133a-5p, IGFBP3 and their interactions in NPC cells. Circ-0046263 was upregulated in both NPC cell lines and tissues. The in vitro functional studies revealed that knockdown of circ-0046263 inhibited the proliferation, invasion, and migration of NPC cells, whereas its overexpression produced the opposite result. In vivo experiments indicated that knockdown or overexpression of circ-0046263 attenuated or promoted tumor growth and metastasis, respectively. Mechanistically, circ-0046263 could act as a miRNA sponge to absorb miR-133a-5p and upregulate the expression of miRNA downstream target IGFBP3. In addition, miR-133a-5p inhibition or IGFBP3 overexpression could rescue the malignant behavior induced by circ-0046263 silencing. Finally, circ-0046263 plays a tumor-promoting role in NPC to enhance malignant behavior through the miR-133a-5p/IGFBP3 axis, which could be a potential target for NPC therapy.

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“…M6A-RNA methylation refers to methylation of adenosine bases at position N6 in 3′UTRs near the stop codons but within the internal long exons [ 10 , 11 ]. Modified IGF2BP2 participates in the development and progression of multiple metabolic disease and cancers, including diabetes [ 2 ], obesity [ 12 ], fatty liver [ 13 ], breast cancer [ 14 ], colorectal carcinoma [ 15 ], esophageal adenocarcinoma [ 16 ], glioma [ 17 ], hepatocellular carcinoma [ 18 ], lung cancer [ 19 ], pancreatic cancer [ 20 ] and many others.…”

Section: Introductionmentioning

confidence: 99%

The role of IGF2BP2, an m6A reader gene, in human metabolic diseases and cancers

2021

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The human insulin-like growth factor 2 (IGF2) mRNA binding proteins 2 (IGF2BP2/IMP2) is an RNA-binding protein that regulates multiple biological processes. Previously, IGF2BP2 was thought to be a type 2 diabetes (T2D)-associated gene. Indeed IGF2BP2 modulates cellular metabolism in human metabolic diseases such as diabetes, obesity and fatty liver through post-transcriptional regulation of numerous genes in multiple cell types. Emerging evidence shows that IGF2BP2 is an N6-methyladenosine (m6A) reader that participates in the development and progression of cancers by communicating with different RNAs such as microRNAs (miRNAs), messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs). Additionally, IGF2BP2 is an independent prognostic factor for multiple cancer types. In this review, we summarize the current knowledge on IGF2BP2 with regard to diverse human metabolic diseases and its potential for cancer prognosis.

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Relationship between <em>IGF2BP2</em> and <em>IGFBP3</em> polymorphisms and susceptibility to non-small-cell lung cancer: a case–control study in Eastern Chinese Han population

Cited by 18 publications

References 36 publications

Identification and validation of m6A RNA methylation regulators with clinical prognostic value in Papillary thyroid cancer

Identification and validation of m6A RNA methylation regulators with clinical prognostic value in Papillary thyroid cancer

Hsa_circ_0046263 functions as a ceRNA to promote nasopharyngeal carcinoma progression by upregulating IGFBP3

The role of IGF2BP2, an m6A reader gene, in human metabolic diseases and cancers

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