Relationship Between Metal Transcription Factor-1 and Zinc in Resistance to Metals Producing Free Radicals

Formigari, Alessia; Alberton, Paolo; Cantale, Vera; Nadal, V. De; Feltrin, Moira; Ferronato, Silvia; Santon, Alessandro; Schiavon, Luca; Irato, Paola

doi:10.2174/2212796810802030256

Cited by 3 publications

(2 citation statements)

References 93 publications

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“…6 The Cu( i ) affinity of the human copper metallochaperone HAH1 is similar to those of the MBDs of the Menkes (MNK) and Wilson (WLN) ATPases, 7 but Zn( ii ) affinities of these proteins have not been reported. MNK 8 and WLN 9 can both bind Zn( ii ). The in vivo (yeast two-hybrid) interaction between HAH1 and the fourth MBD of WLN (WLN4) depends on zinc concentration, and a tight in vitro Zn( ii )-mediated hetero-complex binds Zn( ii ) at sub-nanomolar concentrations.…”

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confidence: 99%

Crosstalk between Cu(i) and Zn(ii) homeostasis via Atx1 and cognate domains

et al. 2013

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Crosstalk between Cu(i) and Zn(ii) homeostasis via Atx1 and cognate domains

et al. 2013

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“…Within the large family of structurally and functionally diverse proteins involved in metal ion metabolism, the most remarkable and extensively studied are metallothioneins and ferritins. Their functions are the maintenance of homeostasis of essential metal ions (Zn 2+ , Cu 2+ and Fe 2+ ), detoxification after increased, acute or chronic exposure to heavy metal ions, and protection against oxidative stress [ 14 , 15 ]. In Drosophila melanogaster , metalothionein genes are expressed differently during development and the abundance of these proteins can be increased by the inductive effect of various metals [ 16 , 17 ]; however, little is known about the influence of thermal stress on their expression, especially in the context of diapause.…”

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Prolonged Heat Stress during Winter Diapause Alters the Expression of Stress-Response Genes in Ostrinia nubilalis (Hbn.)

Uzelac,

Avramov,

Knežić

et al. 2024

IJMS

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During diapause, a state of temporarily arrested development, insects require low winter temperatures to suppress their metabolism, conserve energy stores and acquire cold hardiness. A warmer winter could, thus, reduce diapause incidence and duration in many species, prematurely deplete their energy reserves and compromise post-diapause fitness. In this study, we investigated the combined effects of thermal stress and the diapause program on the expression of selected genes involved in antioxidant defense and heat shock response in the European corn borer Ostrinia nubilalis. By using qRT-PCR, it has been shown that response to chronic heat stress is characterized by raised mRNA levels of grx and trx, two important genes of the antioxidant defense system, as well as of hsp70 and, somewhat, of hsp90, two major heat shock response proteins. On the other hand, the expression of hsc70, hsp20.4 and hsp20.1 was discontinuous in the latter part of diapause, or was strongly controlled by the diapause program and refractory to heat stress, as was the case for mtn and fer, genes encoding two metal storage proteins crucial for metal ion homeostasis. This is the first time that the effects of high winter temperatures have been assessed on cold-hardy diapausing larvae and pupae of this important corn pest.

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Cadmium Induces the Activation, Concentration, and Expression of MTF-1 Protein As a Biomolecular Response of Sea Urchins Deadema setosum

Rumahlatu¹

2017

omni.akua

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Heavy metal cadmium has toxic effects in the bodies of living organisms.However, the bodies of living organisms have the ability to regulate a wide range of toxic entering the body by using biomolecular response that aims at maintaining cell homeostasis. In this research an induction of cadmium in sea urchins Deadema setosum was carried out. Cadmium was induced in 6 levels of concentration in six treatment groups of D. setosum. Each group consisted of 7 individuals.The examination of MTF-1 protein activation was carried out by immunohistochemical method, the measurement of MTF-1 protein concentration was carried out by the indirect ELISA method (Enzyme Linked Imunoasssay) and the examination of MTF-1 protein expression was carried out by western blotting method. The data were analyzed using descriptive analysis and One Way ANOVA followed by Duncan test 0.05 to determine the mean difference of MTF-1 protein concentration of D. setosum. The research results showed that cadmium induction activated MTF-1 characterized by the condition of liver cells of D. setosum which was brown.The higher the exposure of cadmium concentration on D. setosum, the higher the concentration of MTF-1 expressed

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Relationship Between Metal Transcription Factor-1 and Zinc in Resistance to Metals Producing Free Radicals

Cited by 3 publications

References 93 publications

Crosstalk between Cu(i) and Zn(ii) homeostasis via Atx1 and cognate domains

Crosstalk between Cu(i) and Zn(ii) homeostasis via Atx1 and cognate domains

Prolonged Heat Stress during Winter Diapause Alters the Expression of Stress-Response Genes in Ostrinia nubilalis (Hbn.)

Cadmium Induces the Activation, Concentration, and Expression of MTF-1 Protein As a Biomolecular Response of Sea Urchins Deadema setosum

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