Relationship between microbial activity and microbial community structure in six full-scale anaerobic digesters

Regueiro, Leticia; Veiga, Patricia W.; Figueroa, M.; Alonso-Gutierrez, Jorge; Stams, A.J.M.; Lema, Juan M.; Carballa, Marta

doi:10.1016/j.micres.2012.06.002

Cited by 198 publications

(105 citation statements)

References 39 publications

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“…Likewise, apparent methanogenic coefficients increased from 0.37 day Ϫ1 under mesophilic conditions (3) to 0.77 day Ϫ1 for acetoclastic methanogenesis and 1.4 day Ϫ1 for hydrogenotrophic methanogenesis under thermophilic digestion. Uptake on formate (as a proxy for hydrogenotrophic methanogenesis) was higher than on acetate, which is in agreement with previous studies (3,14). The results therefore indicated that hydrolysis and methanogenesis were both improved at 55°C.…”

Section: Discussionsupporting

confidence: 92%

“…The bacterial and archaeal community structures are highly diverse and affected by substrate composition (6,(12)(13)(14), HRT (12), and operating temperature (6, 15). Two methanogenic pathways from acetate have been reported, including (i) direct acetate cleavage in acetoclastic methanogenic archaea, such as Methanosarcina and Methanosaeta, and (ii) nonacetoclastic oxidation, i.e., the syntrophic association of acetateoxidizing organisms and hydrogenotrophic methanogens, including Methanobacteriales (5, 16).…”

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confidence: 99%

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Methanosarcinaceae and Acetate-Oxidizing Pathways Dominate in High-Rate Thermophilic Anaerobic Digestion of Waste-Activated Sludge

Jensen

Batstone

2013

Appl Environ Microbiol

132

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This study investigated the process of high-rate, high-temperature methanogenesis to enable very-high-volume loading during anaerobic digestion of waste-activated sludge. Reducing the hydraulic retention time (HRT) from 15 to 20 days in mesophilic digestion down to 3 days was achievable at a thermophilic temperature (55°C) with stable digester performance and methanogenic activity. A volatile solids (VS) destruction efficiency of 33 to 35% was achieved on waste-activated sludge, comparable to that obtained via mesophilic processes with low organic acid levels (<200 mg/liter chemical oxygen demand [COD]). Methane yield (VS basis) was 150 to 180 liters of CH 4 /kg of VS added . According to 16S rRNA pyrotag sequencing and fluorescence in situ hybridization (FISH), the methanogenic community was dominated by members of the Methanosarcinaceae, which have a high level of metabolic capability, including acetoclastic and hydrogenotrophic methanogenesis. Loss of function at an HRT of 2 days was accompanied by a loss of the methanogens, according to pyrotag sequencing. The two acetate conversion pathways, namely, acetoclastic methanogenesis and syntrophic acetate oxidation, were quantified by stable carbon isotope ratio mass spectrometry. The results showed that the majority of methane was generated by nonacetoclastic pathways, both in the reactors and in off-line batch tests, confirming that syntrophic acetate oxidation is a key pathway at elevated temperatures. The proportion of methane due to acetate cleavage increased later in the batch, and it is likely that stable oxidation in the continuous reactor was maintained by application of the consistently low retention time.A naerobic digestion (AD) is a biological process which has been widely used for waste-activated sludge and primary sludge stabilization. It offers substantial advantages over alternative stabilization techniques, including low energy requirements, good-quality biosolids product, energy recovery in the form of methane (CH 4 ) gas, and the possibility of nutrient recovery (1, 2). Conventional anaerobic digesters are operated at a mesophilic temperature (35°C) with long hydraulic retention times (HRTs) of 15 to 20 days on waste-activated sludge (WAS), which is excess bacterial material and residual organics from combined aerobic treatment of wastewater, and more than 20 days on primary sludge (PS), which is the settled fraction from raw wastewater (1, 3, 4). Long treatment times are a key disadvantage of anaerobic digestion, requiring large tank volumes and, consequently, high capital cost, as well as increased mixing and maintenance costs. Therefore, reducing digester volume would strongly enhance the competitiveness of anaerobic technologies.Thermophilic anaerobic digestion (55°C to 70°C) is an alternative to conventional mesophilic anaerobic digestion (35°C). The majority of systems are operated at 55°C (5, 6), as higher temperatures can result in instability due to ammonia inhibition and reduced operability (2). Thermophilic anaerobic digestion offer...

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Section: Discussionsupporting

confidence: 92%

mentioning

confidence: 99%

Methanosarcinaceae and Acetate-Oxidizing Pathways Dominate in High-Rate Thermophilic Anaerobic Digestion of Waste-Activated Sludge

Jensen

Batstone

2013

Appl Environ Microbiol

132

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“…Genera from the last three major phyla are associated with all steps of the anaerobic digestion process excluding methanogenesis [7], [8]. Different inocula can be obtained from different biogas reactors and it leads to differences in the initial activity, in the substrate adaptation, as well as in the ability to convert the substrate to methane, or to inhibit methanogenesis [9]- [11]. It is suggested for the inoculum to be fresh, homogenous, sieved and preincubated, to have a wide microbial diversity, and to be tested towards model substrates.…”

Section: Introductionmentioning

confidence: 99%

“…Several methods are used to Two main advantages of IR spectroscopy are: 1) the analyzed samples are not subjected to any chemical treatment thus avoiding secondary reactions; 2) all the compounds present in the sample are measured simultaneously, thereby simplifying and speeding up the analysis. The content of the main biochemical components: carbohydrates, proteins, fats, as well as lignin, cellulose etc., can be determined simultaneously from data analysis of one spectrum [9]. Thus, FT-IR spectroscopy is successfully applied for monitoring various biotechnological processes including environmental [21]- [24].…”

Section: Introductionmentioning

confidence: 99%

Application of Anaerobic Digestate as Inoculum for Treatment of the Plant-Derived Wastes: Case Study

Kalneniece¹,

Bērziņš²,

Petrina³

et al. 2017

Materials Science and Applied Chemistry

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-The aim of this study was to characterize the digestate from an activated sludge biogas reactor (IDARS) as an inoculum for enzymatic hydrolysis and biogas production from organic wastes. Aerobic and anaerobic bacteria are represented in IDARS with H' index 3.1 and 2.5, respectively. IDARS stimulated the enzymatic hydrolysis of wood and leaves, preferably broadleaf tree species. The results demonstrated the potential of IDARS for improvement of the technological parameters for conversion of organic wastes into biogas.

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“…High abundance of Syntrophomonas was generally detected with higher archaeal abundance in anaerobic reactors (Regueiro et al, 2012), engaging in obligate syntrophic propionate oxidation (Talbot et al, 2008). Syntrophomonas was also detected with high abundance in anaerobic sludge treating slaughterhouse wastewater with high fats level (Cammarota et al, 2013) and thought to be the major anaerobic bacterium oxidising butyrate and long-chain fatty acids to acetate and hydrogen through β-oxidation (Pereira et al, 2002).…”

Section: Microbial Architecturementioning

confidence: 99%

Microbial Ecology of Fermentative Microbes in Anaerobic Granules

Yang¹

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School of Chemical EngineeringII"|"P a g e " % AbstractGranules are large, self-supporting biofilms that form naturally in high-rate anaerobic treatment systems and are extremely important to reactor functionality. The biofilm structure has functional and phylogenetic layering, important both scientifically and to function.Fermenters, found on the surface of anaerobic granules, are associated with granule strength and uptake of the primary substrate. Compared to acetogens and methanogens, very limited work has been done on fermenters, particularly in granules. Due to their high diversity and difficulty in selective isolation, fermenters are best studied through culture-independent techniques. Until now, the microbial distribution could only be analysed through sectioning and microscopic analysis with fluorescent in situ hybridization. The whole granule could be analysed by DNA extraction and microbial community profiling methods but this did not provide spatial information. This thesis develops a method to remove microbes selectively from successive spatial layers through hydraulic shearing and demonstrates its application on anaerobic granules of three different types (collected from brewery, cannery and dairy wastewater treatment plants). Outer layers, in particular, could be selectively sheared as confirmed by FISH and TRFLP. Further analysis with 454 pyrosequencing showed that a shift in dominant population from presumptive fermenters (such as Bacteroidales and Anaerolinea) in outer layers to syntrophs (such as Syntrophomonas and Geobacter) in inner layers, with progressive changes through the depth. The method was further leveraged through covariance based deep metagenomic sequencing, with metagenomic analysis used so far to align phylogenetic information. This leveraged the shear based method to provide covariance information for the metagenomic analysis. Shear based phylogenetic and metagenomic aligned well internally and with cryosection-FISH analysis. Information provided could be used with more specific probes (particularly Bacteroidales and Anaerolinea) to confirm that these organisms were key fermenters and highly abundant in outer layers. This study indicated that fermenters were a relatively diverse but discrete population within the granule. Further analysis of the metagenomic information is required to identify roles of specific microbes. The phylogenetic approach was also used in a reactor study with different feeds (gelatine, glucose, VFA) to identify how the microbial population shifted during growth of fermenters. While physical strength was not influenced, the III"|"P a g e " % dominant fermentative groups were strongly impacted by the feed matrix, and inner layer community less changed, but still substantially affected.

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Relationship between microbial activity and microbial community structure in six full-scale anaerobic digesters

Cited by 198 publications

References 39 publications

Methanosarcinaceae and Acetate-Oxidizing Pathways Dominate in High-Rate Thermophilic Anaerobic Digestion of Waste-Activated Sludge

Methanosarcinaceae and Acetate-Oxidizing Pathways Dominate in High-Rate Thermophilic Anaerobic Digestion of Waste-Activated Sludge

Application of Anaerobic Digestate as Inoculum for Treatment of the Plant-Derived Wastes: Case Study

Microbial Ecology of Fermentative Microbes in Anaerobic Granules

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