Relationship between organization of the actin cytoskeleton and the cell cycle in normal and adenovirus-infected rat cells

Jackson, Paul; Bellett, A. J. D.

doi:10.1128/jvi.63.1.311-318.1989

Cited by 17 publications

(4 citation statements)

References 22 publications

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“…Such varied activities as genome replication, protein synthesis and transport, and assembly, transport, and release of virions have been associated with these structures. Actin microfilaments in particular have been associated with the rounding and loss of adhesion that frequently occur with viral infection or transformation (6,16,19,24,29,43,47,59), with the intracellular transport of viral structural proteins and viral particles (1,21,49), with the budding process of many enveloped viruses (14,33,34,60), and with the assembly of virions in the cytoplasm (17,44) and in the nucleus (55).…”

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confidence: 99%

Sequential rearrangement and nuclear polymerization of actin in baculovirus-infected Spodoptera frugiperda cells

Charlton

Volkman

1991

J Virol

100

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Proper assembly of nucleocapsids of the baculovirus Autographa californica nuclear polyhedrosis virus is prevented by cytochalasin D, a drug that interferes with actin microfflament function. To investigate the involvement of microfflaments in A. californica nuclear polyhedrosis virus replication, a fluorescence microscopy study was conducted that correlated changes in distribution of microfilaments with events in the life cycle of the virus. Tetramethylrhodamine isothiocyanate-labeled phalloidin was used to label microfflaments, and monoclonal antibody was used to label p39, the major viral capsid protein. Three microfilament arrangements were found in infected cells. During uptake of virus, thick cables were formed. These were insensitive to cycloheximide, indicating that this configuration was a rearrangement of preexisting cellular actin mediated by a component of the viral inoculum. At the time of cell rounding and before viral DNA replication, ventral aggregates of actin were observed. These were sensitive to cycloheximide but not to aphidicolin, indicating that an early viral gene mediated this actin rearrangement. Ventral aggregates did not result from the rounding process itself. Uninfected cells prerounded with colchicine did not form ventral aggregates. Cells prerounded with colchicine and then infected did form aggregates. At the time of exponential production of progeny virus, microfilaments were found in the nucleus surrounding the virogenic stroma. In this area (where nucleocapsid assembly is known to take place) microfilaments colocalized with p39. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western immunoblot analysis identified p39 among proteins retained on an f-actin affinity column. We postulate that microfflaments in the nucleus provide a scaffold to position capsids for proper assembly and filling with DNA.

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confidence: 99%

Sequential rearrangement and nuclear polymerization of actin in baculovirus-infected Spodoptera frugiperda cells

Charlton

Volkman

1991

J Virol

100

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“…In the present study, disruptions in S. littoralis gut morphology and disappearance of microfilament structures in actin ( Figure 6 ) could be a consequence of the phytoecdysteroid titers in the A. iva crude leaf extract. Actin microfilaments in particular have been associated with the rounding and loss of adhesion that frequently occur with viral infection or transformation in response to secondary metabolites [ 51 , 52 ], with the intracellular transport of viral structural proteins and viral particles [ 53 ], with the budding process of many enveloped viruses [ 54 ], and with the assembly of virions in the cytoplasm [ 55 ] and in the nucleus [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Activity of Ajuga iva Extracts Against the African Cotton Leafworm Spodoptera littoralis

Taha-Salaime

Lebedev

Abo-Nassar

et al. 2020

Insects

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Control of the crop pest African cotton leafworm, Spodoptera littoralis (Boisduval), by chemical insecticides has led to serious resistance problems. Ajuga plants contain phytoecdysteroids (arthropod steroid hormone analogs regulating metamorphosis) and clerodanes (diterpenoids exhibiting antifeedant activity). We analyzed these compounds in leaf extracts of the Israeli Ajuga iva L. by liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) and thin-layer chromatography (TLC), and their efficiency at reducing S.littoralis fitness. First and third instars of S. littoralis were fed castor bean leaves (Ricinus communis) smeared with an aqueous suspension of dried methanolic crude extract of A. iva phytoecdysteroids and clerodanes. Mortality, larval weight gain, relative growth rate and survival were compared to feeding on control leaves. We used ‘4’,6-diamidino-2-phenylindole (DAPI, a fluorescent stain) and phalloidin staining to localize A. iva crude leaf extract activity in the insect gut. Ajuga iva crude leaf extract (50, 100 and 250 µg/µL) significantly increased mortality of first-instar S. littoralis (36%, 70%, and 87%, respectively) compared to controls (6%). Third-instar larval weight gain decreased significantly (by 52%, 44% and 30%, respectively), as did relative growth rate (−0.05 g/g per day compared to the relevant controls), ultimately resulting in few survivors. Crude leaf extract (250 µg/µL) reduced gut size, with relocation of nuclei and abnormal actin-filament organization. Ajug iva extract has potential for alternative, environmentally safe insect-pest control.

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“…Histological observations of the gut showed that S. littoralis is Figure 6) could be a consequence of the phytoecdysteroid titers in the A. iva crude leaf extract. Actin microfilaments in particular have been associated with the rounding and loss of adhesion that frequently occur with viral infection or transformation in response to secondary metabolites [56,57], with the intracellular transport of viral structural proteins and viral particles [58], with the budding process of many enveloped viruses [59], and with the assembly of virions in the cytoplasm [60] and in the nucleus [61].…”

Section: Discussionmentioning

confidence: 99%

Biological Activity of <em>Ajuga iva</em> Extracts against the African Cotton Leafworm Spodoptera littoralis

Taha-Salaime

Lebedev

Abo-Nassar

et al. 2020

Preprint

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Control of the crop pest African cotton leafworm Spodoptera littoralis (Boisduval) by chemical insecticides has led to serious resistance problems. Ajuga plants contain phytoecdysteroids (arthropod steroid hormone analogs regulating metamorphoses) and clerodanes (diterpenoids exhibiting antifeedant activity). We analyzed these compounds in leaf extracts of the Israeli Ajuga iva L. by LC-TOF-MS and TLC, and their efficiency at reducing S. littoralis fitness. First and third instars of S. littoralis larvae were fed on castor bean leaves smeared with an aqueous suspension of dried methanolic crude extract of A. iva phytoecdysteroids and clerodanes. Mortality, larval weight gain, relative growth rate and survival were compared to feeding on control leaves. We used DAPI and phalloidin staining to localize A. iva crude leaf extract activity in the insect gut. A. iva crude leaf extract (50, 100 and 250 µg/µL) significantly increased mortality of first-instar S. littoralis larvae (36%, 70% and 87%, respectively) compared to controls (6%). Third-instar larval weight gain decreased significantly (by 52%, 44% and 30%, respectively), as did relative growth rate (–0.05 g/g per day compared to the relevant controls), ultimately resulting in few survivors. Crude leaf extract (250 µg/µL) reduced gut size, with relocation of nuclei and abnormal actin-filament organization. A. iva extract has potential for alternative, environmentally safe insect-pest control.

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Relationship between organization of the actin cytoskeleton and the cell cycle in normal and adenovirus-infected rat cells

Cited by 17 publications

References 22 publications

Sequential rearrangement and nuclear polymerization of actin in baculovirus-infected Spodoptera frugiperda cells

Sequential rearrangement and nuclear polymerization of actin in baculovirus-infected Spodoptera frugiperda cells

Activity of Ajuga iva Extracts Against the African Cotton Leafworm Spodoptera littoralis

Biological Activity of <em>Ajuga iva</em> Extracts against the African Cotton Leafworm Spodoptera littoralis

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