Relationship between ovarian and placental steroid production during early pregnancy in the marmoset monkey (Callithrix jacchus)

Hodges, J. K.; Henderson, Cilla; Hearn, Jack

doi:10.1530/jrf.0.0690613

Cited by 38 publications

(14 citation statements)

References 21 publications

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“…In contrast to that, the strongest signal for cj17HSD7 mRNA was observed in the CL. It increased relative to ␤-actin mRNA in the CL during the first part of pregnancy before the luteo-placental shift in marmoset monkeys [31]. After that, at Day 98, cj17HSD7 expression was reduced.…”

Section: Expression Of Estradiol Synthesizing Enzymes In Reproductivementioning

confidence: 92%

Characterization of 17β-Hydroxysteroid Dehydrogenase Type 7 in Reproductive Tissues of the Marmoset Monkey1

Husen

Adamski

Brüns

et al. 2003

Biology of Reproduction

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In contrast to the known rodent enzymes, the physiological significance of 17␤-hydroxysteroid dehydrogenase type 7 (17HSD7) and its presumed function in reproductive biology is not well understood in primates. As a first step, we recently cloned the complete coding regions of human and marmoset monkey (Callithrix jacchus) 17HSD7 (cj17HSD7). In the present work the complete cDNA of marmoset 17HSD1 (cj17HSD1), including the proximal promoter region, and a partial sequence of marmoset aromatase (cjARO) were sequenced in order to compare the expression of these estradiol synthesizing enzymes with that of 17HSD7 in a primate model and to identify tissues where 17HSD7 might participate in the pathway of estradiol synthesis. The gene structures of cj17HSD1 and cj17HSD7 were determined and proved to be very similar to the human orthologues. Northern hybridization showed that cjARO mRNA seems to be coexpressed preferably with cj17HSD1 in placenta, whereas in other tissues it is expressed in parallel only with cj17HSD7. Especially in corpora lutea, the cj17HSD7 transcript is detectable throughout the luteal phase of the ovarian cycle and increases during pregnancy, in parallel with the transcript of aromatase. Results were confirmed by immunoblots and immunohistochemistry using new polyclonal antisera directed against cj17HSD7 and cjARO protein. The enzymatic conversion of estrone to estradiol was assessed in marmoset corpora lutea. The pattern of coexpression with aromatase supports the hypothesis that luteal 17HSD7 complements placental 17HSD1, ensuring continued estradiol synthesis throughout pregnancy in primates.corpus luteum function, estradiol, female reproductive tract, ovulatory cycle, pregnancy

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Section: Expression Of Estradiol Synthesizing Enzymes In Reproductivementioning

confidence: 92%

Characterization of 17β-Hydroxysteroid Dehydrogenase Type 7 in Reproductive Tissues of the Marmoset Monkey1

Husen

Adamski

Brüns

et al. 2003

Biology of Reproduction

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“…Luteolysis was defined for luteal phases and pregnancies as return of follicularphase levels of urinary EIC within days 2 and 8 following treatment. Given that the placenta assumes the major role of steroid secretion at about day SO (Hodges et al 1983b), luteolysis was unlikely to have been the cause of abortion in the small proportion of cloprostenol treatments performed around and after day 50. For convenience only, therefore, all cloprostenol treatments that resulted in the termination of pregnancy are called luteolytic below.…”

Section: Cloprostenol Treatmentmentioning

confidence: 99%

Monitoring and controlling reproduction in captive common marmosets on the basis of urinary oestrogen metabolites

Nievergelt

Pryce²

1996

Lab Anim

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SummaryNon-invasive methods for routine monitoring of reproductive states and reproduction control in large colonies of captive Collithrix iacchus have been developed. Immunoactive urinary oestrone-3-conjugates (E]C) were measured during non-conception cycles (n=S) and pregnancy In=7). Using plasma progesterone levels to time ovulation, ovulation was quantitatively estimated by al calculating the first EtC rise and b) by establishing an E]C threshold. Ovulation was thus defined as taking place 4 days preceding al the first rise of E]C above follicular-phase levels, or b) a concentration~4.5 J.lgE]C/mg creatinine. Early pregnancy could be determined after day 20 by continued luteal-phase levels of E]C. Secondly, the luteolytic effect of cloprostenol, injected over a wide range of doses and between days 1 and 64 after ovulation/ conception, was analysed. Luteolysis was achieved when cloprostenol was administered after day 5 post-ovulation; the luteolytic effect was found not to be dose-dependent. The success of cloprostenol treatment was 87% as confirmed by endocrine monitoring. The methods described are effective and minimize intervention, and are therefore suitable for long-term applications, particularly in combination with behavioural studies. KeywordsCommon marmoset, urinary oestrogen metabolites, timing of ovulation, determination of pregnancy, luteolytic agent, controlling reproduction Common marmosets (Callithrix iacchus) are important laboratory primates. They are used widely in research on immunology, toxicology, virology, behaviour and reproduction (e.g. Hearn 1994). Marmosets have many advantages as laboratory research primates: they have small body size and are easy to manage; they breed well in captivity, showing no seasonal limitations and usually producing twins twice a year (Hearn 1983). The routine monitoring of ovulation and pregnancy in marmosets is often essential: for example, to regulate the rate of reproduction in a large colony and for studies in many aspects of

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“…Plasma progesterone levels were measured using radioimmunoassay (Diagnostic Product Corporation Pty. Ltd., Los Angeles, California) to monitor the reproductive cycles (Harlow et al, 1984;Hodges et al, 1983) using femoral vein blood samples (0.3 ml) taken initially twice weekly. The marmoset has approximately an 8 day follicular phase and a 20 day luteal phase (Hearn, 1983).…”

Section: Materials and Methods Tracking Reproductive Cyclesmentioning

confidence: 99%

Ultrastructural studies of glycan changes in the apical surface of the uterine epithelium during pre-ovulatory and pre-implantation stages in the marmoset monkey

1999

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It has been postulated that carbohydrates are involved in a variety of cell-cell interactions including blastocyst implantation. In primates, there are only limited investigations on the ultrastructural localisation of the cyclic changes in uterine epithelial surface carbohydrates. Our aim was to investigate such changes during the pre-ovulatory and pre-implantation stages of the reproductive cycle in the marmoset monkey.After fixation of endometrial tissues, avidin-ferritin lectin cytochemistry was employed for apical surface glycan detection at the ultrastructural level. Five lectins were used including Canavalia ensiformis (Con A), Lotus tetragonolobus (LTA), Glycine max (SBA), Phytolacca americana (PWM) and Triticum vulgaris (WGA). Morphometry was used to quantitate changes in the intensity of lectin staining by determining the total number of ferritin particles per unit length of membrane. Surface and intra-cytoplasmic vesicles, stained by the lectins, were also examined.Quantitative ferritin assessment showed that 1 day before presumed implantation (days 11 to 12 after ovulation in the marmoset monkey) there was a significant increase in Con A, LTA and SBA staining on the apical uterine epithelial plasma membrane compared to the pre-ovulatory phase and earlier stages of pregnancy (days 4-8 after ovulation). A significant increase in PWM was also detected from early pregnancy to preimplantation stages. All lectins except WGA produced reproducible staining within reproductive cycle groups. The greatest variation and intensity of epithelial surface staining was observed with WGA and the weakest with LTA. The patchy staining with LTA compared with thick coverage by WGA indicated the complexity of the carbohydrate arrangement in the glycocalyx of the uterine surface plasma membrane. Reduction of WGA reactivity after neuraminidase treatment suggested that the lectin binding might be related to the presence of heavily sialylated apical uterine membrane glycoconjugates.This is the first high-resolution study in primates to report quantitative cyclic changes in fucosyl, galactosyl, glucosyl, and mannosyl sugar residues of the apical uterine epithelial glycocalyx. The findings support the concept Abbreviations: DC, NЈN diacetyl chitobiose; Fuc, fucose; Glc, glucose; Man, mannose; GalNAc, N-acetyl galactosamine; GlcNAc, N-acetyl glucosamine; NeuNAc, neuraminic acid (sialyl residues); NЈdase, neuraminidase; sWGA, succinyl wheatgerm; WGA, native wheatgerm; nil, not applied; 0 to 7, lectin staining on a scale from 0 (negative) to 7 (heavy).

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Relationship between ovarian and placental steroid production during early pregnancy in the marmoset monkey (Callithrix jacchus)

Cited by 38 publications

References 21 publications

Characterization of 17β-Hydroxysteroid Dehydrogenase Type 7 in Reproductive Tissues of the Marmoset Monkey1

Characterization of 17β-Hydroxysteroid Dehydrogenase Type 7 in Reproductive Tissues of the Marmoset Monkey1

Monitoring and controlling reproduction in captive common marmosets on the basis of urinary oestrogen metabolites

Ultrastructural studies of glycan changes in the apical surface of the uterine epithelium during pre-ovulatory and pre-implantation stages in the marmoset monkey

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