Relationship between phosphates and alkaline phosphatase of Anabaena flos-aquae in continuous culture

Bone, Derek H.

doi:10.1007/bf00411879

Cited by 51 publications

(15 citation statements)

References 15 publications

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“…In the P-limited chemostat, enzyme activity incrcascd with the degree of P limitation, as noted also by Fuhs et al ( 1972) and Bone ( 1971) . Alkaline phosphatase activity associated with the phytoplankton in the central North Pacific was occasionally detcctcd in waters of the mixed layer (Table 8; Perry 1972).…”

Section: Resultssupporting

confidence: 69%

Phosphate utilization by an oceanic diatom in phosphorus‐limited chemostat culture and in the oligotrophic waters of the central North Pacific1

Perry

1976

Limnology & Oceanography

295

150

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Thalassiosira pseudonana from the oligotrophic waters of the central North Pacific Ocean was grown in phosphorus-limited chemostat culture. When the chemical composition of the cells, expressed as ratios, was compared with that of nitrogen-limited cultures, several of these ratios varied in excess of a factor of five between the two systems. Among these, which emerged as diagnostic indicators of phosphorus-starvation vs. nitrogen-deficiency, were the C : P, N : P, P : Chl a and Chl a : ATP ratios.The physiological responses of a population were determined by its preconditioning history. The minimum cellular phosphorus content, go, varied with the degree of phosphorus starvation. The rate of phosphate uptake at a saturating concentration of phosphate by phosphorus-limited cultures of T. pseudonana exceeded the rates of uptake by nonphosphorus-limited cultures by an order of magnitude; the K, for phosphate uptake was not a function of phosphorus deficiency.Phytoplankton in phosphorus-starved chemostat cultures on medium made with central North Pacific Ocean water did not USC naturally occurring dissolved organic phosphorus despite the presence of cell-surface alkaline phosphatase. Phosphate uptake by natural phytoplankton assemblages in the central North Pacific was measured with JaP. Uptake rates in the field were not significantly affected by light intensity. No circadian periodicity in uptake rates was detected in natural phytoplankton or algae cultured on a light/dark cycle.In the midninetcenth century von Licbig formulated the principle that the maximum population size or maximum yield of plant material was controlled by a single factor, such as "a" limiting nutrient. Brandt (cite&! in Gran 1912) observed that this principle also applied to the regulation of phytoplankton organic production by soluble nutrients. Under specified conditions in a batch culture in the laboratory, which can be considered as analogous to the agronomist's cxpcrimcntal plot, a maximum population density of algae can easily be reached.

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Section: Resultssupporting

confidence: 69%

Phosphate utilization by an oceanic diatom in phosphorus‐limited chemostat culture and in the oligotrophic waters of the central North Pacific1

Perry

1976

Limnology & Oceanography

295

150

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“…At least at intermediate to high dilution rates, diazotrophically growing cultures contained less biomass than the NH,+ cultures (Table l ) , as has been observed before for AnabuenaJlos-aquae (Bone 1971, Rhee andLederman 1983, Layzell et al 1985). This lowered biomass concentration is a consequence of both a reduced yield on P (Yp; Fig.…”

Section: Discussionsupporting

confidence: 74%

INTERACTION OF NITROGEN FIXATION AND PHOSPHORUS LIMITATION IN APHANIZOMENON FLOS‐AQUAE (CYANOPHYCEAE)¹

Nobel

Snoep

Westerhoff

et al. 1997

Journal of Phycology

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The effect of simultaneous nitrogen jixation and phosphorus limitation on the physiological adaptation and growth performance of Aphanizomenon flos-aquae (L.) Ralfs PCC 7905 was studied in continuous culture. I n the absence of ammonia, N2jixation occurred and the maximum growth rate (as determined in diluted batch cultures) was lower. However, no distinction could be made between the steady-state N uptake rates (based on cellular N contents) of N,-Jxing cells and cells grown with ammonia. At the higher dilution rates, the residual P concentration increased with increasing dilution rate, more so under N2fixing conditions, compared to the cultures grown in the presence of ammonia. More generally, the yield of biomass per consumed P, as the biomass concentration itseg decreased with increasing dilution rate, and both were lower under N,-jixing conditions. The restricted biomass production under N2-jixing conditions suggests that reduction of N loading may benefit lake restoration projects. The injuence of N2-Jxation on the sevm't~ of P limitation is discussed in terms of metabolic control analysis. From the increase of the residual P concentration on switching from ammonium to N,-jixing conditions, it is deduced that under N,-jixing and P-limited conditions, control of growth is shared by N and P metabolism.Restoration of eutrophic freshwater lakes by reducing the phosphorus (P) load does not always result in a significant reduction of phytoplankton biomass (e.g. Van Liere and Janse 1992). It was suggested that an additional reduction of the nitrogen (N) load might lead to better (i.e. quicker) results (Lijklema 1983). A complicating factor however could be the occurrence of the cyanobacterial, filamentous species of the order Nostocales, potentially I

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“…Assays for chorismate mutase and phenylalanine aminotransferase were performed on extracts from both halves of the culture to compare specific activities under the presumed repressed and derepressed conditions (Table I). The specific activity of alkaline phosphatase was also examined in both extracts using the method of Bone (1971) as a control for protein degradation under conditions of starvations.…”

Section: R E S U L T S a N D Discussionmentioning

confidence: 99%

Absence of Repression in a Phenylalanine Auxotroph of Synechococcus cedrorum

Kaney¹,

Jhabvala²

1975

Journal of General Microbiology

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Relationship between phosphates and alkaline phosphatase of Anabaena flos-aquae in continuous culture

Cited by 51 publications

References 15 publications

Phosphate utilization by an oceanic diatom in phosphorus‐limited chemostat culture and in the oligotrophic waters of the central North Pacific1

Phosphate utilization by an oceanic diatom in phosphorus‐limited chemostat culture and in the oligotrophic waters of the central North Pacific1

INTERACTION OF NITROGEN FIXATION AND PHOSPHORUS LIMITATION IN APHANIZOMENON FLOS‐AQUAE (CYANOPHYCEAE)¹

Absence of Repression in a Phenylalanine Auxotroph of Synechococcus cedrorum

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Relationship between phosphates and alkaline phosphatase of Anabaena flos-aquae in continuous culture

Cited by 51 publications

References 15 publications

Phosphate utilization by an oceanic diatom in phosphorus‐limited chemostat culture and in the oligotrophic waters of the central North Pacific1

Phosphate utilization by an oceanic diatom in phosphorus‐limited chemostat culture and in the oligotrophic waters of the central North Pacific1

INTERACTION OF NITROGEN FIXATION AND PHOSPHORUS LIMITATION IN APHANIZOMENON FLOS‐AQUAE (CYANOPHYCEAE)1

Absence of Repression in a Phenylalanine Auxotroph of Synechococcus cedrorum

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INTERACTION OF NITROGEN FIXATION AND PHOSPHORUS LIMITATION IN APHANIZOMENON FLOS‐AQUAE (CYANOPHYCEAE)¹