Relationship Between Physiological Status and Serum Constituent Values in Minke Whales (Balaenoptera acutorostrata).

Fukui, Yutaka; Mogoe, Toshihiro; Terawaki, Y.; Ishikawa, Hajime; Fujise, Yoshihiro; Ohsumi, Seiji

doi:10.1262/jrd.41.203

Cited by 4 publications

(5 citation statements)

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“…The cleavage rate after ICSI was 46.2%, and a total of six embryos developed to the 2-cell (n = 3), 4-cell (n = 1) or 5-cell (n = 2) stages (Fig. The sodium concentration of minke whale serum (179 mmol/l) (Fukui et al, 1995) is higher than those of porcine (147 mmol/l) (Chang et al, 1976) and mouse serum (148 mmol/l) (Roblero et al, 1976), and the osmolarity of minke whale follicular fluid (388 mosmol) was also higher than those of porcine and bovine follicular fluid (approximately 300 mosmol) (Iwayama et al, 2005). The nuclei of a 5-cell stage embryo are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Major improvement of the IVM method in the present study resulted from changing the osmolarity of the IVM medium to 390 mosmol and supplementing the IVM medium with whale follicular fluid instead of fetal whale serum. The sodium concentration of minke whale serum (179 mmol/l) (Fukui et al, 1995) is higher than those of porcine (147 mmol/l) (Chang et al, 1976) and mouse serum (148 mmol/l) (Roblero et al, 1976), and the osmolarity of minke whale follicular fluid (388 mosmol) was also higher than those of porcine and bovine follicular fluid (approximately 300 mosmol) (Iwayama et al, 2005). The high sodium concentration in the serum and high osmolarity in the follicular fluid are probably specific characteristics of marine mammals.…”

Section: Resultsmentioning

confidence: 99%

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Developmental capacity of Antarctic minke whale (Balaenoptera bonaerensis) vitrified oocytes following in vitro maturation, and parthenogenetic activation or intracytoplasmic sperm injection

Fujihira

Kobayashi

Hochi

et al. 2006

Zygote

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The present study investigated the effects of the sexual maturity of oocyte donors on in vitro maturation (IVM) and the parthenogenetic developmental capacity of fresh minke whale oocytes. The effects of cytochalasin B (CB) pretreatment and two types of cryoprotectant solutions (ethylene glycol (EG) or ethylene glycol and dimethylsulfoxide (EG + DMSO)) on the in vitro maturation of vitrified immature whale oocytes were compared, and the developmental capacity of vitrified immature whale oocytes following IVM and intracytoplasmic sperm injection examined (ICSI). The maturation rate did not differ significantly with sexual maturity (adult, 60.9%; prepubertal, 53.1%), but the parthenogenetic activation rate of oocytes from adult donors (76.7%) was significantly higher (p < 0.05) than that of oocytes from prepubertal donors (46.4%). The maturation rates after vitrification and warming were not significantly different between the EG (22.2%) and EG + DMSO groups (30.2%), or between the CB-treated (30.4%) and non-CB-treated groups (27.3%). These results indicate that parthenogenetic activation of in vitro matured oocytes from adult minke whales was superior to that from prepubertal whales, but that the developmental capacity of the whale oocytes after parthenogenetic activation or ICSI was still low. The present study also showed that CB treatment before vitrification and two kinds of cryoprotectants did not improve the IVM rate following the vitrification of immature whale oocytes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Developmental capacity of Antarctic minke whale (Balaenoptera bonaerensis) vitrified oocytes following in vitro maturation, and parthenogenetic activation or intracytoplasmic sperm injection

Fujihira

Kobayashi

Hochi

et al. 2006

Zygote

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“…Fujihira et al . (2006) increased the osmolarity of the medium by addition of NaCl, KCl, MgSO 4 and CaCl 2 in medium 199 at a constant ratio may be due to higher concentration of sodium in Antarctic minke whale serum (179 mmol) (Fukui et al ., 1995) than those of porcine (147 mmol) (Chang et al ., 1976) and mouse serum (148 mmol) (Roblero et al ., 1976). Contrasting to the previous study, we increased the osmolarity of medium by addition of sorbitol in the present study because reduced concentration of sodium chloride has been recommended for the development of bovine embryos in vitro (Lim et al ., 1994a; Liu & Foote, 1996).…”

Section: Discussionmentioning

confidence: 98%

“…The importance of NaCl concentration and osmolarity of culture media for optimum development of preimplantation embryos has been documented in the bovine (Lim et al, 1994a;Liu & Foote, 1996), porcine (Beckmann & Day, 1993), mouse (Lawittis & Biggeres, 1992;Biggers et al, 1993), rabbit (Li & Foote, 1996) and rat (Miyoshi et al, 1994). Fujihira et al (2006) increased the osmolarity of the medium by addition of NaCl, KCl, MgSO 4 and CaCl 2 in medium 199 at a constant ratio may be due to higher concentration of sodium in Antarctic minke whale serum (179 mmol) (Fukui et al, 1995) than those of porcine (147 mmol) (Chang et al, 1976) and mouse serum (148 mmol) (Roblero et al, 1976). Contrasting to the previous study, we increased the osmolarity of medium by addition of sorbitol in the present study because reduced concentration of sodium chloride has been recommended for the development of bovine embryos in vitro (Lim et al, 1994a;Liu & Foote, 1996).…”

Section: Discussionmentioning

confidence: 99%

Attempts atin vitrofertilization and culture ofin vitromatured oocytes in sei (Balaenoptera borealis) and Bryde's (B. edeni) whales

Bhuiyan

Suzuki

Watanabe

et al. 2009

Zygote

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The cumulus-oocyte-complexes (COCs) recovery rates with respect to reproductive status per sei (Balaenoptera borealis) and Bryde's (B. edeni) whales were determined in Experiment 1. The number of COCs recovered ranged from 16.0 to 30.6 and from 6.7 to 26.8 per sei and Bryde's whales, respectively. The effects of COCs grades and protein supplementation in embryo culture medium on development of in vitro fertilized (IVF) embryos were evaluated in sei and Bryde's whales in Experiment 2. The COCs were classified into either Grade A (COCs with five or more layers of compact cumulus cells) or Grade B (COCs with less than five layers of compact or expanded cumulus cells) before being cultured for IVM. The cleavage (12.0 to 19.5%), 4-cell (8.0 to 12.0%) and 8-cell (4.0 to 8.0%) formation rates in sei whales did not vary significantly between embryos derived from either grade A or B oocytes and between embryos cultured in either fetal whale serum (FWS)- or bovine serum albumin (BSA)-supplemented medium. The cleavage (4.0 to 14.8%), 4-cell (0.0 to 7.5%) and 8-cell (0.0 to 2.6%) formation rates in Bryde's whales did not vary significantly between embryos derived from either grade A or B oocytes and between embryos cultured in either FWS- or BSA-supplemented medium. The grade B oocytes cultured in FWS-supplemented medium developed to morula stage (1.1%) in sei whales. In conclusion, the present study indicates that IVF in sei whales is possible to achieve cleaved embryos developing to morula stage. This is the first in vitro embryo production attempt in sei and Bryde's whales.

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“…In regard to whales, Fukui et al [23] and Suzuki et al [24] have previously measured the plasma or serum concentrations of gonadotropins and steroid hormones in pregnant and non-pregnant Antarctic minke whales. However, the concentrations of the chemical components of wFF from follicles of different sizes have not been investigated in detail, except for some steroid hormones such as progesterone (P 4 ), testesterone (T) and estradiol 17-β (E 2 ) [25].…”

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confidence: 99%

Follicle Size-Dependent Changes in Follicular Fluid Components and Oocyte Diameter in Antarctic Minke Whales (Balaenoptera bonaerensis)

Nagai

Mogoe²,

Ishikawa³

et al. 2007

Journal of Reproduction and Development

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Abstract. The concentrations of various components of follicular fluid were compared among three groups of follicles (small, <5 mm; medium: 5-10 mm; large, >10 mm) with a control that consisted of the components of umbilical serum using seven pregnant Antarctic minke whales. Follicular oocytes recovered from the follicles were also used for measurement of oocyte diameter after removing the cumulus cells. The mean diameter of the ooplasm in the oocytes from the large follicles (143.2 µm) was significantly greater than those from the small (127.1 µm) and medium (131.7 µm) follicles, although there were no significant differences in diameter of the whole oocyte and thickness of the zona pellucida among the three follicular sizes. The osmolarity of the follicular fluid from the small follicles (363.3 mOsmol) was significantly lower than that of the medium follicles (388.9 mOsmol) and tended to be lower than that of large (381.9 mOsmol) follicles, respectively, both of which were similar to that of the umbilical serum (379.5 mOsmol). There was no significant difference in the concentrations of all components of the follicular fluid between the medium and large follicles. As compared with the values of the umbilical serum, the total-protein, glucose, albumin and chlorine concentrations of the follicular fluid from the medium and large follicles were significantly higher, and the total cholesterol and calcium concentrations were significantly lower. The concentrations of lactic acid (85.3-136.0 mg/dl) of the follicular fluid from the three groups of follicles were significantly lower than that of the umbilical serum (360.0 mg/dl). Among the follicles, the follicular fluid from the small follicles (136.0 mg/dl) contained a significantly higher concentration of lactic acid than that from the large follicles (85.3 mg/dl). The progesterone concentrations were not significantly different among the fluid from the three group of follicles and the umbilical serum: however, the estradiol 17-β concentrations of the follicular fluid increased with the size of the follicle (14.3 and 34.6 ng/ml for small and large follicles, respectively). These results offer new information concerning whale reproductive physiology, especially for improvement of in vitro oocyte maturation and related technologies in whales.

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Relationship Between Physiological Status and Serum Constituent Values in Minke Whales (Balaenoptera acutorostrata).

Cited by 4 publications

References 10 publications

Developmental capacity of Antarctic minke whale (Balaenoptera bonaerensis) vitrified oocytes following in vitro maturation, and parthenogenetic activation or intracytoplasmic sperm injection

Developmental capacity of Antarctic minke whale (Balaenoptera bonaerensis) vitrified oocytes following in vitro maturation, and parthenogenetic activation or intracytoplasmic sperm injection

Attempts atin vitrofertilization and culture ofin vitromatured oocytes in sei (Balaenoptera borealis) and Bryde's (B. edeni) whales

Follicle Size-Dependent Changes in Follicular Fluid Components and Oocyte Diameter in Antarctic Minke Whales (Balaenoptera bonaerensis)

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