Our objective was to examine the effects of processing, moisture, and anaerobic storage length of reconstituted corn grain on the fermentation profile, geometric mean particle size and ruminal DM disappearance. Dry corn kernels were ground (hammer mill, 5-mm screen) or rolled, then rehydrated to 30, 35 or 40% moisture, and stored for 0, 14, 30, 60, 90, 120 or 180 d in laboratory silos. Rolled corn had an increased geometric mean particle size compared with ground corn (2.24 and 1.13 mm, respectively, at ensiling). However, there was a trend for an interaction between processing and moisture concentration to affect particle size, with geometric mean particle size increasing with increased moisture concentration, especially in ground corn. Longer storage periods also slightly increased geometric mean particle size. Processing, moisture, and storage length interacted to affect the fermentation pattern (two- or three-way interactions). Overall, pH decreased, whereas lactic acid, acetic acid, ethanol, and NH3-N increased with storage length. Reconstituted corn grain with 30% moisture had less lactic acid than corn with 35% and 40% moisture, indicating that fermentation might have been curtailed, and also due to the clostridial fermentation that converts lactic acid to butyric acid. Ensiling reconstituted ground corn with 30% of moisture led to greater concentrations of ethanol and butyric acid, resulting in greater dry matter loss than grain rehydrated to 35 or 40% of moisture. Ammonia-N and in situ ruminal dry matter (DM) disappearance were highest for reconstituted ground corn with 35 or 40% of moisture, mainly after 60 d of storage. Therefore, longer storage periods and greater moisture contents did not offset negative effect of greater particle size on the in situ ruminal DM disappearance of rolled reconstituted corn grain. Nonetheless, reconstituted corn grain should be ensiled with more than 30% moisture and stored for at least two months to improve the ruminal DM disappearance and reduce the formation of ethanol and butyric acid.