Relationship between production of acute-phase proteins and strength of inflammatory stimulation in rats

Kuribayashi, Takashi; Tomizawa, Misaki; Seita, Tetsurou; Tagata, K.; Yamamoto, Seigo

doi:10.1258/la.2011.010112

Cited by 19 publications

(25 citation statements)

References 23 publications

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“…First, the expression of AGP is regulated by both cytokines (interleukin-1, interleukin-6 and tumor necrosis factorα) and glucocorticoids, unlike other acute phase proteins including fibrinogen, ceruloplasmin and α 2 -microglobulin, which only by interleukin-6. [11][12][13][14] The regulation of AGP production in human hepatocytes by glucagon, interleukin-8 and the interleukin-6 is thought to act via mitogen-activated protein kinase (MAPK) pathway. [15] Furthermore, endogenous and exogenous AGP was found to present at sites of inflammation in rats with inflammatory granuloma in a study using fluorescent labeled antibody to AGP or iodine125 labeled AGP.…”

Section: Introductionmentioning

confidence: 99%

Molecular Aspects of Human Alpha-1 Acid Glycoprotein — Structure and Function

Taguchi¹,

Nishi²,

Chuang³

et al. 2013

Acute Phase Proteins

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Section: Introductionmentioning

confidence: 99%

Molecular Aspects of Human Alpha-1 Acid Glycoprotein — Structure and Function

Taguchi¹,

Nishi²,

Chuang³

et al. 2013

Acute Phase Proteins

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“…Moreover, serum concentrations of α2M increased in rats injected the mixture of IL-6 and CINC-1 rich fraction separated from serum obtained from rats during acute inflammation. Then, IL-6 and CINC-1 are considered to contribute the production of α2M in rats [ 4 5 6 7 ]. α2M was produced by the liver and the serum concentrations of α2M decreased in impairment liver rats [ 8 ].…”

mentioning

confidence: 99%

Elimination half-lives of interleukin-6 and cytokine-induced neutrophil chemoattractant-1 synthesized in response to inflammatory stimulation in rats

Kuribayashi

2018

Lab Anim Res

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The elimination half-lives of in Interleukin-6 (IL-6) and cytokine-induced neutrophil chemoattractant-1 (CINC-1) in rats after inflammatory stimulation were investigated. Five male Sprague-Dawley rats were used (age, 9 weeks; body weight, 235–375 g). Turpentine oil was intramuscularly injected at a dose of 2 mL/kg body weight to induce acute inflammation. Blood was collected pre-injection and 6, 12, 24, 36, 48, 60, 72, 84, and 96 h after the turpentine oil injection. Serum concentrations of IL-6, CINC-1, and α2-macroglobulin (α2M) were measured by enzyme-linked immunosorbent assay. Half-lives were calculated as 0.693/elimination rate constant. The serum concentration of α2M peaked at 48 h after turpentine oil injection. Serum concentrations of IL-6 and CINC-1 increased and peaked at 12 and 24 h, respectively. The terminal elimination half-lives of IL-6 and CINC-1 were 15.5 and 29.9 h, respectively. The half-life of CINC-1 was significantly longer than that of IL-6 (P=0.006). These results suggested that these cytokines synthesized in response to inflammatory stimulation were rapidly eliminated in rats. The serum concentrations of these cytokines should be measured at an early stage if these cytokines will be used as surrogate inflammatory markers instead of acute-phase proteins.

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“…We found a positive correlation between KIM and 170-kDa protein, which could correspond to alpha-2 macroglobulin in rats. Alpha-2-macroglobulin is a typical acute-phase protein in rats [ 17 , 18 ]. In our study groups, KIM-1 levels were higher in rats treated with CyA and lower in rats treated with rapamycin.…”

Section: Discussionmentioning

confidence: 99%

Effect of Immunosuppressive Therapy on Proteinogram in Rats

et al. 2016

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BackgroundIt has been observed that the use of immunosuppressive drugs in patients after transplantation of vascularized organs may be associated with changes in the concentration of certain fractions of plasma proteins. The concentration of these proteins was correlated with an increased risk of occurrence of stage 3 chronic kidney disease (CKD). This article examines the effect of the most commonly used immunosuppressive drugs on the concentration of plasma proteins in Wistar rats.Material/methodsThe study involved 36 rats grouped according to the immunosuppressive regimen used (tacrolimus, mycophenolate mofetil, cyclosporine A, rapamycin, and prednisone). The rats in all study groups were treated with a 3-drug protocol for 6 months. The treatment dose was adjusted based on available data in the literature. No drugs were administered to the control group. The rats were sacrificed and blood samples collected to determine the concentration of plasma proteins using electrophoresis technique.ResultsStatistically significant differences were observed between protein concentrations within the studied groups. The differences related to the proteins with masses of 195 kDa, 170 kDa, 103 kDa, and 58 kDa.Conclusions(1) Immunosuppressive drugs caused changes in the proteinogram of plasma proteins. (2) The strongest effect on rat plasma proteins was exerted by a regimen based on rapamycin. Intermediate, weak, and weakest effects were observed in regimens based on cyclosporine A, tacrolimus, and mycophenolate mofetil, respectively.

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Relationship between production of acute-phase proteins and strength of inflammatory stimulation in rats

Cited by 19 publications

References 23 publications

Molecular Aspects of Human Alpha-1 Acid Glycoprotein — Structure and Function

Molecular Aspects of Human Alpha-1 Acid Glycoprotein — Structure and Function

Elimination half-lives of interleukin-6 and cytokine-induced neutrophil chemoattractant-1 synthesized in response to inflammatory stimulation in rats

Effect of Immunosuppressive Therapy on Proteinogram in Rats

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