Relationship between the mRNA expression levels of calpains 1/2 and proteins involved in cytoskeleton remodeling

Abstract

Remodeling of the cytoskeleton underlies various cellular processes, including those associated with metastasis. The role of the proteases and proteins involved in cytoskeletal reorganization is being actively studied. However, there are no published data on the relationship between the mRNA expression levels of calpains 1/2 (CAPN 1/2) and the proteins associated with cytoskeleton remodeling. Therefore, the purpose of our study was to establish the relationship between the mRNA expression levels of CAPN 1/2 and the proteins involved in cytoskeletal reorganization, such as cell motility markers (SNAI1, VIM, and RND3) and actin-binding proteins (CFN1, PFN1, EZR, FSCN1, and CAP1) using the model of laryngeal/laryngopharyngeal squamous cell carcinoma (LC). The gene expression level was determined by reverse transcriptase real-time PCR and calculated using the 2-Ct method in paired tissue samples of 44 patients with LC (T1-4N0-2M0). The patients were divided into two groups: those with low and those with high CAPN 1/2 expression levels. It was found that metastasis in LC patients was associated with decreased expression levels of VIM and CAP1, and increased levels of CAPN1. A high level of CAPN2 was accompanied by a high expression level of EZR, indicating the activation of invasion processes. The results obtained need to be confirmed in further studies using a larger sample of patients and target genes. Our study is important in elucidating the mechanisms that underlie cancer progression and metastasis, a development that could subsequently open the way to a search for new prognostic and predictive markers of laryngeal/laryngopharyngeal cancer progression.

Human Amniotic Epithelial Cells as a Tool to Investigate the Effects of Cyanidin 3-O-Glucoside on Cell Differentiation

“…CAPN2 encodes calpain-2 catalytic subunit, which is a calcium-regulated non-lysosomal thiol-protease. It catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction [ 40 ]. STMN1 encodes Stathmin, which is involved in the regulation of the microtubule filament system by destabilizing microtubules.…”

Serum levels of cytoskeleton remodeling proteins and their mRNA expression in tumor tissue of metastatic laryngeal and hypopharyngeal cancers

“…Switching between EMT and MAT can be quite fast and depends on the properties of the microenvironment. The participation of proteins of the Rho family GTPases is common to all migration routes [6,7,8,9]. The model of the mouse glioblastoma cell line showed that the induced expression of Rnd3 (Rho family-GTPases) inhibited the activity of Snail1, which blocked the migration of tumor cells [10].…”

miR‑140‑5p alleviates mouse liver ischemia/reperfusion injury by targeting CAPN1

“…CAPN1 belongs to the calpains family, and along with other members, is responsible for limited proteolysis of a variety of target substrates (39). Under physiological conditions, calpains are involved in numerous processes such as cytoskeleton remodeling, signal transduction pathway regulation, platelet activation, cell differentiation, and cell apoptosis (39)(40)(41). CAPN1 overactivation results in unregulated proteolysis and aberrant activation of signaling cascades, leading to cellular damage and ultimately cell death (42).…”