Relationship of Glycolytic and Oxidative Metabolism to Particle Entry and Destruction in Phagocytosing Cells

Selvaraj, Ratnam J.; Sbarra, Anthony J.

doi:10.1038/2111272a0

Cited by 180 publications

(60 citation statements)

References 12 publications

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“…This hypothesis predicts that light emission should not occur with granulocytes stimulated by a nonparticulate agent. Fluoride is such an agent, since it has been shown to be capable of stimulating the cellular oxidative burst (30,31), resulting in the production of large quantities of superoxide (32). In Fig.…”

Section: Methodsmentioning

confidence: 99%

The origin of the chemiluminescence of phagocytosing granulocytes.

Cheson¹,

Christensen²,

Sperling³

et al. 1976

J. Clin. Invest.

259

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Section: Methodsmentioning

confidence: 99%

The origin of the chemiluminescence of phagocytosing granulocytes.

Cheson¹,

Christensen²,

Sperling³

et al. 1976

J. Clin. Invest.

259

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“…The effect of F-on the burst was studied further by Selvaraj and Sbarra (9), who concluded that it acted by precipitating with other ions within the cell as an insoluble particle that induced the burst. However, the evidence that fluoride-treated neutrophils contained internalized material was inconclusive.…”

Section: Introductionmentioning

confidence: 99%

Fluoride-mediated activation of the respiratory burst in human neutrophils. A reversible process.

Curnutte¹,

Babior²,

Karnovsky³

1979

J. Clin. Invest.

157

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“…human adult serum (Animal Blood Center, Syracuse, N.Y.) to give final concentrations of leucocytes at 1 0 7 per ml and spores at 1 0 8 per ml. Control suspensions were then compared with leucocytespore suspension to which the following inhibitors were separately added: Glyceraldehyde (final concentration of 8 mM) was added to the first test-tube concurrent with the addition of 4sCa-labelled spores to inhibit leucocytic glycolysis and, ultimately, phagocytosis (Selvaraj and Sbarra, 1966). Colchicine (0.04 mM) was introduced to a second leucocyte-spore suspension.…”

Section: Phagocytosis Systems With Metabolic Inhibitorsmentioning

confidence: 99%

A Study Of Metabolic Factors Involved In The Intracellular Germination Of Clostridium Botulinum Spores After Phagocytosis

Suzuki

Grecz

1972

Journal of Medical Microbiology

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T H E chemical environment within the metabolically active leucocyte during the phagocytic act is conducive to intracellular spore germination. This leucocyte-induced germination of Clostridium botulinum type A spores has been studied in various in-vitro systems after Booth, Suzuki and Grecz (1971a) reported that heat-resistant spores of this organism are converted to heatsensitive germinated spores and vegetative cells in the peritoneal cavity after they have been injected intraperitoneally (IP) into mice.These workers used new staining techniques (Booth, Suzuki and Grecz, 19716) to demonstrate phagocytosis by leucocytes in vivo and in vitro and to show subsequent germination of the spores within the leucocyte. Furthermore, Suzuki, Booth and Grecz (1970) recently found that Cl. botulinum spores must be actively phagocytosed to release toxin. The production of an equivalent ionic environment or exposure of the spores to leucocyte cytoplasmic extracts did not, by themselves, induce spore germination or toxin release. Studies involving the implantation of Millipore diffusion chambers containing spores alone or spores plus leucocytes into the peritoneal cavity of guinea-pigs further supported this concept (Suzuki, Booth, Benedik and Grecz, 1971). Ten per cent. of the animals given chambers containing spores alone died with typical symptoms of botulism, whereas chambers containing spores plus leucocytes killed 90 per cent. of the test guinea-pigs. Passively immunised guinea-pigs were able to survive the implantation of chambers containing C Z . botulinum spores plus leucocytes. Thus, it has been established that polymorphonuclear (PMN) leucocytes can provoke a pathogenic state when interacting with Cl. botulinum spores.During phagocytic engulfment, lactic acid accumulates as a result of glycolysis and causes a rapid decrease in pH of the phagocytic vacuole (Cohn, 1968) to values as low aspH 3.0-4-5 (Rous, 1925;Rowley, 1962). The pentose phosphate pathway present in peripheral leucocytes (Bona, Macovschi and Gheordunescu, 1969 ;Morton, Moran and Stjernholm, 1969) generates its metabolic by-product H202 and brings the reaction of the phagocytic vacuole to near neutrality. Concurrently, lysosome degranulation proceeds and brings into the proximity of the phagocytic vacuole a multitude of enzymes that include lysozyme and myeloperoxidase; the latter forms an effective bactericidal complex with H202 (Klebanoff, 1968(Klebanoff, , 1970Paul et al., 1970).The purpose of the present study was to determine the relationship of metabolic processes within the PMN leucocyte to the induction of intracellular CZ. botulinum spore germination and subsequent toxin release.

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Relationship of Glycolytic and Oxidative Metabolism to Particle Entry and Destruction in Phagocytosing Cells

Cited by 180 publications

References 12 publications

The origin of the chemiluminescence of phagocytosing granulocytes.

The origin of the chemiluminescence of phagocytosing granulocytes.

Fluoride-mediated activation of the respiratory burst in human neutrophils. A reversible process.

A Study Of Metabolic Factors Involved In The Intracellular Germination Of Clostridium Botulinum Spores After Phagocytosis

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