Relationship of microbial mass and activity in biodegradation of solid waste

Murphy, Richard J.; Jones, David; Stessel, Richard Ian

doi:10.1016/s0734-242x(05)80026-6

Cited by 16 publications

(11 citation statements)

References 19 publications

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“…(g dry wt.) 31 of cellulolytic bacteria at di¡erent depths of the land¢ll site of Aveley (Essex, UK) and by Palmisano et al [11] who did not detect any cellulolytic bacteria in Fresh Kill Land¢ll (New York, USA). In these previous studies, cellulolytic bacteria were numerated by inoculating the refuse extract directly on cellulose-containing medium and incubating plates under anaerobic condition.…”

Section: Discussionmentioning

confidence: 95%

“…Microbial degradation of cellulose appears as an important metabolic activity in land¢ll where cellulose and hemicellulose are considered to represent 91% of the methane potential of fresh refuse according to Barlaz et al [29]. Previous studies which concerned cellulolytic bacteria of land¢ll have focused only on counting bacteria [8,10^11] or on measuring global cellulolytic activity [7,10,30,31]. To our knowledge, phenotypic studies of cellulolytic bacteria of MSW have been restricted to the identi¢cation of one strain of Cellulomonas [12] and to the characterization of nine anaerobic cellulolytic bacterial isolates [15].…”

Section: Discussionmentioning

confidence: 99%

“…(g dry wt.) 31 . Cellulolytic bacteria were present in all refuse samples and ranged from 1.1U10 6 to 2.3U10 8 c.f.u.…”

Section: Microbiological Countsmentioning

confidence: 99%

“…(g dry wt.) 31 according to the sample ( Table 2). The proportion of cellulolytic bacteria to the total culturable heterotrophic bacteria varied accord- ing to samples, ranging from 3.7 to 75%.…”

Section: Microbiological Countsmentioning

confidence: 99%

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Enumeration and characterization of cellulolytic bacteria from refuse of a landfill

Pourcher¹

2001

FEMS Microbiology Ecology

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Enumeration and phenotypic characterization of aerobic cellulolytic bacteria were performed on fresh, 1 year old and 5 years old refuse samples of a French landfill site. Numbers of cellulolytic bacteria ranged from 1.1U10 6 to 2.3U10 8 c.f.u. (g dry wt.) 31 and were lower in 5 years old refuse samples. A numerical analysis of phenotypic data based on 80 biochemical tests and performed on 321 Gram-positive isolates from refuse, revealed a high phenotypic diversity of cellulolytic bacteria which were distributed into 21 clusters. Based on the phenotypic analysis and the sequencing of 16S rDNA of five representative strains of major clusters, the predominant cellulolytic groups could be assigned to the family of Bacillaceae and to the genera Cellulomonas, Microbacterium and Lactobacillus. Furthermore, chemical parameters such as pH, carbohydrates and volatile solid contents influenced the composition of the cellulolytic bacterial groups which were reduced essentially to the family of Bacillaceae in the oldest refuse samples. ß

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

“…(g dry wt.) 31 . Cellulolytic bacteria were present in all refuse samples and ranged from 1.1U10 6 to 2.3U10 8 c.f.u.…”

Section: Microbiological Countsmentioning

confidence: 99%

Section: Microbiological Countsmentioning

confidence: 99%

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Enumeration and characterization of cellulolytic bacteria from refuse of a landfill

Pourcher¹

2001

FEMS Microbiology Ecology

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“…Some of the most common methods for characterizing the quality of compost during processing include an analysis of caloric content, carbon content, and biological oxygen demand [8,35,36]. A diversity of methods also exist for monitoring the progression of polysaccharide degradation including chemical quantification of different polysaccharides, assays of cellulolytic and amylolytic activities in the waste stream, isotopic tracking of labeled organics, and NMR and NIR spectroscopy [5,6,32,[37][38][39]. Such methods offer a snapshot of the overall abundance of different materials in compost, and the biochemical dynamics of the process, but they provide insufficient information regarding the degree of decomposition relative to progression to an endpoint as decomposition is occurring.…”

Section: Discussionmentioning

confidence: 99%

Endpoint Fragmentation Index: A Method for Monitoring the Evolution of Microbial Degradation of Polysaccharide Feedstocks

Green¹,

Popa

2010

Appl Biochem Biotechnol

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We describe a simple method for tracking the course of microbial degradation of polysaccharide-rich feedstocks. The method involves determining total polysaccharides present in the feedstock, measured in glucose equivalents, relative to the fractional component of polysaccharides exhibiting 2,3-dinitrosalycylic acid aldehyde activity. The ratio of total polysaccharide to aldehyde activity, defined as the end-point fragmentation (EPF) index, is then calculated and tracked as it shifts as microbial degradation of polysaccharide-rich feedstock progresses. While degradation occurs, the EPF index falls. It bottoms out at an asymptotic limit marking the point in time where further degradation of the polysaccharide-rich feedstock has ceased. The EPF index can be used to follow the progressive breakdown of composting polysaccharide-rich waste. It may also have applicability as a means of tracking the turnover of polysaccharides in other complex environments including soil, sediments, wetlands, and peat bogs.

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Application of hydrogeological and biological research for the lysimeter experiment performance under simulated municipal landfill condition

Dąbrowska

Sołtysiak

Biniecka

et al. 2019

J Mater Cycles Waste Manag

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The size and chemical composition of leachates migrating into the aquifer are dependent on the parameters of the waste and the storage conditions. Lysimeter studies allow us to determine the size and chemical composition of leachates as well as the leachate water balance. Lysimeter studies were conducted on a 230-L municipal waste sample for 6 months. During the tests, the specific electrolyte conductivity, pH, Eh, and temperature, as well as the chemical composition, microbiological analysis, and profiling of physiological population level using EcoPlate™ microarrays were measured in collected leachate samples. During the entire experiment, the amounts of inflow and outflow from lysimeters were measured. To assess the existence of significant differences in the chemical component concentrations in leachates, use of Principal Component Analysis was taken into account. The maximum EC value from leachate from the lysimeter was 33 mS/cm. High concentrations of ammonium ion (up to approx. 1400 mg dm −3), chlorides (up to approx. 6800 mg dm −3), and iron (up to approx. 31 mg dm −3) were observed in the effluents. The number of enterococci in May reached 53,000 cells/100 ml. By contrast, the number of these microorganisms was about 15,000 and 16,000 CFU/100 ml in January and April, respectively. Community-level physiological profiling indicates that the activity and functional diversity of microorganisms were higher in the leachate samples obtained in winter compared to effluents collected from lysimeters in spring.

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Relationship of microbial mass and activity in biodegradation of solid waste

Cited by 16 publications

References 19 publications

Enumeration and characterization of cellulolytic bacteria from refuse of a landfill

Enumeration and characterization of cellulolytic bacteria from refuse of a landfill

Endpoint Fragmentation Index: A Method for Monitoring the Evolution of Microbial Degradation of Polysaccharide Feedstocks

Application of hydrogeological and biological research for the lysimeter experiment performance under simulated municipal landfill condition

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