Relationship of nitrate supply with growth rate, plasma membrane‐bound and cytosolic nitrate reductase, and tissue nitrate content in tobacco plants

Stöhr, Christine

doi:10.1046/j.1365-3040.1999.00389.x

Cited by 44 publications

(30 citation statements)

References 44 publications

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“…A reduction in NUE has also been observed in some C 4 species such as Miscanthus × giganteus (Danalatos et al, 2007). Furthermore, some studies have reported that excessive application of NO 3 − can reduce leaf area in Nicotiana tabacum (Stöhr, 1999), and whole plant growth in some other species such as Brassica campestris L., Brassica chinensis var. Oleifera Makino et Nenotohas and Spinacia oleracia L. (Chen et al, 2004).…”

Section: Introductionmentioning

confidence: 91%

“…Moreover, concentrations around 30 mM of N or higher have been used in plant culture, either as control (Ciompi et al, 1996) or as high N concentration (Marín et al, 2011). were replaced by CaCl 2 , CaSO 4 and K 2 SO 4 , according to Reed and Hageman (1980) and Stöhr (1999). A minimum of five plants per treatment was used and the study was repeated six times.…”

Section: Growth Chamber Experimentsmentioning

confidence: 99%

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High nitrate supply reduces growth in maize, from cell to whole plant

Saiz-Fernández

Diego

Sampedro

et al. 2015

Journal of Plant Physiology

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Section: Introductionmentioning

confidence: 91%

Section: Growth Chamber Experimentsmentioning

confidence: 99%

High nitrate supply reduces growth in maize, from cell to whole plant

Saiz-Fernández

Diego

Sampedro

et al. 2015

Journal of Plant Physiology

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“…Stöhr (1999) (Table 2) documented the mostly superoptimum N level in hydroponics but not in sand cultures (Plhák 1994(Plhák , 1996.…”

Section: Discussionmentioning

confidence: 99%

Nitrogen supply through transpiration mass flow can limit nitrogen nutrition of plants

Plhák¹

2003

PLANT SOIL ENVIRON

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Pea (Pisum sativum L.), sunflower (Helianthus annuus L.) and maize (Zea mays L.) plants were cultivated for 10 days in hydroponics at 1mM and 7mM nitrate or ammonium concentrations at regulated pH 6 and ambient CO 2 level. Plant growth, content of total N and both ions in plant tissues, uptake of water and both N ions were evaluated, N uptake related to transpiration mass flow and to diffusion supply was calculated. Pea and sunflower preferred nitrate nutrition while maize plants used both N ions. The content of total N as well as of both N ions in plant tissues increased with N level with some exceptions. The uptake of both N ions related to transpiration mass flow was dependent on transpiration rate and N ion concentration. At a 1mM N concentration the uptake of N ions related to transpiration mass flow was low and reached in maize up to 16 times, in sunflower 11 times and in pea 2-3 times lower values in comparison with diffusion supply. At a 7mM N concentration N uptake in pea plants was totally supplied by transpiration mass flow, in sunflower plants the ratio of N supply related to transpiration mass flow amounted to 50% and in maize plants N supply through diffusion prevailed, amounting to 70-80%. These results explicate N starvation at low N supply that can intensify at elevated CO 2 causing decreased stomatal diffusion. The acquisition of inorganic nitrogen is considered as one of the most important aspects of plant mineral nutrition. Plants take up nitrogen mainly in the form of nitrate and ammonium. Differences in the uptake and assimilation of both these N ions in different kinds of plants were the topic of numerous papers published in the past (Haynes and Goh 1978) as well as recently (Matt et al. 2001). The occurrence of both N ions in plant stands depends on natural processes taking place in soils and on different anthropogenic activities as well, especially agricultural ones with the aim to intensify plant production.In the last decade numerous studies were devoted to the clarification of plant behaviour (Drake et al. 1997) and to the description of interactions between photosynthesis, metabolism, growth and nitrogen supply at elevated CO 2 (Geiger et al. 1999, Stitt and Krapp 1999, Maroco et al. 2002.The main goal of this study is to compare the uptake of NO 3 -and NH 4 + at different N levels and constant pH value of nutrient solution in pea, sunflower and maize. Plant growth, N content, N uptake and N supply to roots by diffusion and transpiration mass flow were investigated. The acquired results are discussed also with respect to N supply at elevated CO 2 . MATERIAL AND METHODSThree plant species were used for the experiments: pea (Pisum sativum L.) cv. Liliput, sunflower (Helianthus annuus L.) cv. Albena and maize (Zea mays L.) hybrid CE 205. Plant seeds were germinated for three days in vermiculite moistened with distilled water at 25°C before being placed to hydroponics. Each treatment consisted of 32 plants and each treatment was placed in a container with 4 l of nutrient solution...

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“…The reduction of plant growth in N60 ( Figure 1B) was probably due to a detrimental effect of excess nitrate supply as observed in N. tabacum (Stöhr 1999). In this regard, CO 2 enrichment may provide a solution to mitigate the reduction of growth without decreasing N and/or protein accumulation per unit of biomass, because such a treatment was reported to promote growth without decreasing protein content per unit of fresh weight in N. tabacum (Geiger et al 1999).…”

mentioning

confidence: 83%

Effect of nitrate concentration in nutrient solution on hemagglutinin content of Nicotiana benthamiana leaves in a viral vector-mediated transient gene expression system

Fujiuchi

Matsuda

Matoba

et al. 2014

Plant Biotechnology

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Viral vector-mediated transient expression enables rapid biopharmaceutical protein production using whole plants. In recent years, plant growth conditions have received close attention as factors influencing the content of recombinant protein. In particular, because nitrogen is an important resource used in protein synthesis, nitrogen supplied to the root zone may influence accumulation of recombinant proteins. We tested whether fertilization of Nicotiana benthamiana with high-nitrate nutrient solution during plant growth prior to Agrobacterium-mediated delivery (agroinfiltration) of a tobamoviral vector resulted in high recombinant influenza hemagglutinin (HA) content per unit of leaf fresh weight and/or per plant. Nutrient solution containing 60 mM nitrate yielded 40% higher HA content per unit of leaf fresh weight, and comparable HA content per plant, relative to nutrient solution containing 12 or 36 mM nitrate. There were positive correlations among HA, total soluble protein (TSP), and soluble reduced-nitrogen content, suggesting that fertilization with the high-nitrate nutrient solution increased soluble reduced nitrogen and subsequently provided high TSP and HA. Although the increased HA content per unit of leaf fresh weight was offset by reduced leaf fresh weight on a per plant basis, nitrate-enriched fertilization represents an easily applicable technique to obtain a given amount of HA from smaller leaf biomass, thereby potentially reducing downstream processing costs for HA vaccine production using viral vector-mediated transient expression systems.

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Relationship of nitrate supply with growth rate, plasma membrane‐bound and cytosolic nitrate reductase, and tissue nitrate content in tobacco plants

Cited by 44 publications

References 44 publications

High nitrate supply reduces growth in maize, from cell to whole plant

High nitrate supply reduces growth in maize, from cell to whole plant

Nitrogen supply through transpiration mass flow can limit nitrogen nutrition of plants

Effect of nitrate concentration in nutrient solution on hemagglutinin content of Nicotiana benthamiana leaves in a viral vector-mediated transient gene expression system

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