Relationship of ROS accumulation and superoxide dismutase isozymes in developing anther with floret fertility of rice under heat stress

Zhao, Qian; Zhou, Lujian; Liu, Jianchao; Du, Xiaoxia; Asad, Muhammad‐Asad‐Ullah; Huang, Fudeng; Pan, Gang; Cheng, Fangmin

doi:10.1016/j.plaphy.2017.11.009

Cited by 105 publications

(89 citation statements)

References 35 publications

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“…Trxs usually work in a complex with several other proteins involved in ROS scavenging (Yoshida et al ., ; Zhang et al ., ). In addition to Trxs, antioxidant enzymes, predominantly superoxide dismutase (SOD) and catalase (CAT), also help to reduce cellular ROS levels in rice, particularly under high temperature stress, thus avoiding male sterility (Zhao et al ., ,). Several mutants defective in ROS homeostasis displayed developmental defects and sterility mainly due to ROS accumulation (Hu et al ., ; Heng et al ., ).…”

Section: Introductionmentioning

confidence: 99%

DEGENERATED PANICLE AND PARTIAL STERILITY 1 (DPS1) encodes a cystathionine β‐synthase domain containing protein required for anther cuticle and panicle development in rice

et al. 2019

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Summary Degeneration of apical spikelets and reduced panicle fertility are common reasons for low seed‐setting rate in rice (Oryza sativa). However, little is known about the underlying molecular mechanisms. Here, we report a novel degenerated panicle and partial sterility 1 (dps1) mutant that showed panicle apical degeneration and reduced fertility in middle spikelets. dps1 plants were characterized by small whitish anthers with altered cuticle morphology and absence of pollen grains. Amounts of cuticular wax and cutin were significantly reduced in dps1 anthers. Panicles of dps1 plants showed an accumulation of reactive oxygen species (ROS), lower antioxidant activity, and increased programmed cell death. Map‐based cloning revealed that DPS1 encodes a mitochondrial‐localized protein containing a cystathionine β‐synthase domain that showed the highest expression in panicles and anthers. DPS1 physically interacted with mitochondrial thioredoxin proteins Trx1 and Trx20, and it participated in ROS scavenging. Global gene expression analysis in dps1 revealed that biological processes related to fatty acid metabolism and ROS homeostasis were significantly affected, and the expression of key genes involved in wax and cutin biosynthesis were downregulated. These results suggest that DPS1 plays a vital role in regulating ROS homeostasis, anther cuticle formation, and panicle development in rice.

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Section: Introductionmentioning

confidence: 99%

DEGENERATED PANICLE AND PARTIAL STERILITY 1 (DPS1) encodes a cystathionine β‐synthase domain containing protein required for anther cuticle and panicle development in rice

et al. 2019

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“…Under different abiotic stresses including drought, the production of ROS is increased many fold, which leads to oxidative stress in plants [1,30]. Higher ROS levels induce lipid peroxidation in plants and cause injury to cell membranes [10,15]. In this study, we observed a sharp increase in H 2 O 2 and MDA levels under drought stress in WT plants, but H 2 O 2 and MDA levels were significantly less in OsHSFA3-overexpressing plants ( Figure 5).…”

Section: Discussionmentioning

confidence: 48%

“…The antioxidant defense system is one of the major tolerance mechanisms for ROS scavenging under abiotic stresses [14]. Enzymatic antioxidants including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) are key ROS-scavenging enzymes [15,16]. Similarly, polyamines are vital plant metabolites that serve as important ROS scavengers and also induce stomatal closure under drought stress [17,18].…”

mentioning

confidence: 99%

Rice OsHSFA3 Gene Improves Drought Tolerance by Modulating Polyamine Biosynthesis Depending on Abscisic Acid and ROS Levels

Zhu

Zhang

Gao

et al. 2020

IJMS

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Drought is a serious problem, which causes heavy yield losses for rice. Heat-shock factors (HSFs) had been implicated in tolerance to drought and high temperature. However, there has not been much functional characterization and mechanism clarification in rice. Previously, we found an HSF gene, OsHSFA3, was highly related with drought tolerance after screening from 10,000 different samples. Herein, we cloned the OsHSFA3 from rice and overexpressed it in Arabidopsis thaliana to study its regulatory mechanism of drought tolerance. Phenotypic and physiological assays of the transgenic Arabidopsis lines showed that overexpression of OsHSFA3 confers drought tolerance by reducing water loss and reactive oxygen species (ROS) levels, whereas it increases abscisic acid (ABA) levels. However, enzymatic antioxidants such as activity levels of superoxide dismutase, peroxidase and catalase were not significantly different between wild type and transgenic lines. Instead, we observed a significant increase in polyamine content, which was correlated with increased AtADC1, AtADC2, SPDS1 and SPMS expression levels. In silico and in vivo analyses confirmed that OsHSFA3 is a nuclear-localized gene. In addition, OsHSFA3 can bind to the promoter of AtADC1 and OsADC via a yeast one-hybrid assay. Overall, this study reveals that OsHSFA3 improves drought tolerance in Arabidopsis not only by increasing ABA levels, but also by modulating polyamine levels to maintain ROS homeostasis, therefore it could be a strong candidate to develop drought-tolerant rice cultivars.Since plants cannot move from one place to other to avoid the harmful effects of stresses, they adopt various mechanisms for stress tolerance. The antioxidant defense system is one of the major tolerance mechanisms for ROS scavenging under abiotic stresses [14]. Enzymatic antioxidants including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) are key ROS-scavenging enzymes [15,16]. Similarly, polyamines are vital plant metabolites that serve as important ROS scavengers and also induce stomatal closure under drought stress [17,18]. The enzyme arginine decarboxylase (ADC) catalyzes arginine to agmatine, which is the precursor of putrescine and higher polyamines (PAs), such as spermidine and spermine. Several genes have been reported to control the production of polyamines in plants [18,19]. In Arabidopsis thaliana, ADC only has two paralogues, AtADC1 and AtADC2 [20]. In rice, several genes regulate the biosynthesis of polyamines in a collective way and contribute to drought tolerance [21,22]. Thus, exploiting such tolerance mechanisms provide an amazing option to cope with drought stress in rice.Transcription factors (TFs) are well-known for their role in abiotic stress tolerance in plants [8,23]. Among the different TF families, heat shock factors (HSFs), WRKY-motif containing proteins (WRKY), myeloblastosis (MYB) and APETALA2/ethylene response factor (AP2/ERF) families are the most promising for their role in drought and other abiotic stress tolerance [18,[24][25]...

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“…Higher expression of these genes is linked with improved heat tolerance in rice (Zhao et al . 2018a; Zhao et al . 2018b; Das et al .…”

Section: Discussionmentioning

confidence: 99%

“…Rice SOD and CAT are key stress responsive genes which regulate the level of SOD and CAT enzymes in rice (Zhao et al . 2018a; Zhao et al . 2018b).…”

Section: Discussionmentioning

confidence: 99%

Agronomic, physiological and molecular characterization of rice mutants revealed key role of ROS and catalase in high temperature stress tolerance

Zafar

Hameed

Ashraf

et al. 2019

Preprint

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Plants adapt to harsh environments particularly high temperature stress by regulating their physiological and biochemical processes, which are key tolerance mechanisms. Thus, identification of heat-tolerant rice genotypes and reliable selection indices are crucial for rice improvement programs. Here, we evaluated the response of a rice mutant population for high-temperature stress at the seedling and reproductive stages based on agronomic, physiological and molecular traits. The estimate of variance components revealed significant differences (P<0.001) among genotypes, treatments and their interaction for almost all traits. Principal component analysis showed significant diversity among the genotypes and traits under high-temperature stress. The mutant ‘HTT-121’ was identified as the most heat tolerant mutant with higher grain yield, panicle fertility, cell membrane thermo-stability (CMTS) and antioxidant enzyme levels under heat stress conditions. Various seedling-based morpho-physiological traits (leaf fresh weight, relative water contents, malondialdehyde, CMTS) and biochemical traits (superoxide dismutase, catalase and hydrogen peroxide) explained variations in grain yield that could be used as selection indices for heat tolerance in rice at early growth stages. Notably, heat sensitive mutants showed a significant accumulation of ROS level, reduced activities of catalase and upregulation of OsSRFP1 expression under heat stress, suggesting their key role in regulating heat tolerance in rice. The heat-tolerant mutants identified in this study could be used in breeding programs and the development of mapping populations to unravel the underlying genetic architecture for heat-stress adaptability.Summary text for table of contentsHeat stress probably due to changing climate scenario has become a serious threat for global rice production. On the other side, efforts to develop high yielding cultivars have led to the reduced genetic variability to withstand harsh environmental conditions. This study aimed to identify novel heat tolerant mutants developed through gamma irradiation which will provide a unique genetic resource for breeding programs. Further, we have identified reliable selection indices for screening heat-tolerant rice germplasm at early growth stages.

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Relationship of ROS accumulation and superoxide dismutase isozymes in developing anther with floret fertility of rice under heat stress

Cited by 105 publications

References 35 publications

DEGENERATED PANICLE AND PARTIAL STERILITY 1 (DPS1) encodes a cystathionine β‐synthase domain containing protein required for anther cuticle and panicle development in rice

DEGENERATED PANICLE AND PARTIAL STERILITY 1 (DPS1) encodes a cystathionine β‐synthase domain containing protein required for anther cuticle and panicle development in rice

Rice OsHSFA3 Gene Improves Drought Tolerance by Modulating Polyamine Biosynthesis Depending on Abscisic Acid and ROS Levels

Agronomic, physiological and molecular characterization of rice mutants revealed key role of ROS and catalase in high temperature stress tolerance

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