Relationship of sperm superoxide dismutase-like activity with other sperm-specific enzymes and experimentally induced lipid peroxidation in infertile men

Abstract

Key words. Spermatozoa induced lipid peroxidationsuperoxide dismutase-like activityisoenzyme LDH-C4 -diaphoraseexperimentally I Summary. Superoxide dismutase-like activity (SOD-like), isoenzyme lactate dehydrogenase-C4 (LDH-C4) and NADH-diaphorase activities in spermatozoa have been investigated from 58 normozoospermic and 27 oligozoospermic men.Significantly higher SOD-like, LDH-C4 and diaphorase activities (P<O.01, P<0.005 and P< 0.0001, respectively) were detected in spermatozoa from oligozoospermic men, compared to the activities found in normozoospermic samples.SOD-like activity (mean & SE) in oligozoospermic samples amounted to 8.3f 1.6 U spermatozoa, while in spermatozoa in normozoospermic men with a sperm concentration above 20 million of spermatozoa per ml amounted to 4.2 k0.5 U There was a close correlation between the SOD-like activity and biochemical indicators of the presence of residual cytoplasm i.e. isoenzyme LDH-C4 and NADH-diaphorase (r = 0.53 and r = 0.66 in normozoospermic and r=0.63 and r = 0.54 in oligozoospermic men, respectively). A positive relationship between SOD-like activity and experimentally-induced lipid peroxidation was detected in 54 infertile men (r=0.30; P<0.05). These findings suggest that a higher level of superoxide dismutase-like activity may reflect a defect in the development or maturation of spermatozoa and, thereby, a decreased fertility potential. Hence, determination of SOD-like activity may give information on the state of maturity of human spermatozoa, while its role in the antioxidative protection remains to be determined.

Semen quality of honey bee drones maintained from emergence to sexual maturity under laboratory, semi-field and field conditions

“…Superoxide dismutase is one of the antioxidant enzymes involved in the survival of drone spermatozoa (Wegener et al 2012). SOD level has an essential role in maintaining the balance between reactive oxygen species (ROS) generation and degradation (Gavella et al 1996). Kurpisz et al (1996) demonstrated that SOD activity has a beneficial impact on sperm movement, which depends on the dynein ATPase that hydrolyzes ATP to produce a flagellar beat.…”

Low density lipoprotein on poor quality mithun (Bos frontalis) semen preservation

“…During preservation, the semen is exposed to cold shock at atmospheric oxygen which in turn increases the susceptibility to lipid peroxidation due to higher production of reactive oxygen species thereby inducing ageing of sperm and affecting the preservation of semen (Maxwell and Stojanov, 1996). Lipid peroxidation ultimately leads to the impairment of sperm function, altered sperm motility, membrane integrity and damage of sperm DNA and lowered fertility (Gavella et al, 1996). Aitken and Clarkson (1987) reported the axoneme and mitochondria in sperms may be damaged by a high level of reactive oxygen species and MDA.…”

Increase Activity of Superoxide Dismutase in Adults SpragueDawley Rats Liver After Exposed To Bisphenol A

“…In addition, increased superoxide activity has been shown to play an important part in the pathogenesis of different genetic and acquired forms of hypertension in experimental animals, such as, spontaneously hypertensive rats [19][20][21] , angiosensin-infused rats 22,23 , salt-sensitive Dahl rats 24 and lead-exposed rats 25 . On the other hand over expression of SOD may reflect a defect in the development or maturation of spermatozoa, as well as sperm cellular damage, resulting in decreased sperm fertilization potential 26,27 ; knowing that spermatozoa are avoid of cytoplasmic enzyme systems that are required to repair the damage induced by oxidative stress 28 . SOD-2 is located in mitochondria and belongs to a family superoxide dismutase enzymes converts superoxide radicals to H 2 O 2 , which is subsequently converted by catalase or glutathione peroxidase to H 2 O [29][30][31] .…”