Relationship of the bovine IGF1, TG, DGAT1 and MYF5 genes to meat colour, tenderness and cooking loss

Abstract

Bovine insulin-like growth factor 1 (IGF1), thyroglobulin (TG), diacylglycerol-O-acyltransferase 1 (DGAT1) and myogenic factor 5 (MYF5) genes play an important role in the physiology of lipid and muscle metabolism and are therefore considered as candidate genes for meat production traits in farm animals. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in IGF1, TG, DGAT1 and MYF5 genes and to evaluate whether these polymorphisms affected meat colour, tenderness and cooking loss in Holstein cattle. Initially, the SNPs were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. Meat samples (N= 50) derived from M. longissimus thoracis et lumborum (LTL) were used in the current study. Significant differences in variations of meat colour parameters were observed at 24 hours post-mortem. IGF1 was associated with colour parameters of a* and chroma values. In addition, effects of TG were statistically significant on L* and a* values, while, effects of MYF5 were significant on a* value. There was no association of the tested SNPs with meat pH, tenderness and cooking loss. The results presented here may give the valuable information for improving meat colour in cattle.

Association of DGAT1 With Cattle, Buffalo, Goat, and Sheep Milk and Meat Production Traits

“…Lysine (K)→ alanine (A) was detected on exon 8 of DGAT1 gene of Bos taurus: (Figure 3) (21,22,45). Later on, many studies documented the significant association of DGAT1 K232A polymorphism with milk production traits in dairy cattle (45)(46)(47)(48)(49). The DGAT1 region analysis in six Indian cattle (Sahiwal, Rathi, Deoni, Tharparkar, Red Kandhari, and Punganur) revealed fixed DGAT1 K allele (Figure 3) (45).…”

“…Many studies have investigated the effect of DGAT1 gene on meat production traits in beef cattle (4,5,48,131). The role of DGAT1 gene for production traits has also been reported in seven cattle breeds (Angus, Belgian Blue, Charolais, Hereford, Holstein-Friesian, Limousin, and Simmental cattle) (132).…”

Genotyping in the Insulin-like Growth Factor 1 (IGF1/SnaBI) gene of Pasundan cattle with PCR-RFLP method

“…In addition, CC genotype in Polish Holstein had higest of body weight at two months and average daily gain from one to two months (Szewczuk et al 2013). Ardicli et al (2018) reported that the CC genotype had the best of meat colour score at 24 hours post-slaughtering in FH bull. The CC genotype in the present study was detected in all samples and can be suggested as the common genotype for Pasundan cattle.…”

“…Polymorphism in IGF1/SnaBI was affected to the productivity traits of cattle i.e. body weight at three months in Hanwoo cattle (Chung & Kim 2005), serum concentration of IGF1 at 14 days post partum in Friesian Holstein (FH) cattle (Mirzaei et al 2012), service per conception and days open in FH cattle (Ararouti et al 2013), breeding value of milk and fat yields in Iranian Holstein bulls (Mehmannavaz et al 2010), growth traits in beef cattle (Siadkowska et al 2006;Reyna et al 2010;Szewczuk et al 2013), carcass traits (Reyna et al 2010) and meat colour in FH bulls (Ardicli et al 2018). This research was aimed to detect SNP in the exon 1 of IGF1/SnaBI gene in Pasundan cattle using PCR-RFLP method.…”

Effect of the bovine TG5 gene polymorphism on milk- and meat-producing ability

“…The thyroglobulin 5′ leader sequence (TG5) gene is one of the longest mammalian genes. In cattle, it is located in the centromere region of the 14 th chromosome and consists of 37 exons [6][7][8][9]. The TG5 gene has two allelic variants, TG5 T and TG5 C , and three genotypes, TG5 CC , TG5 CT , and TG5 TT .…”