Relationship of Tumoral Hyaluronic Acid and Cathepsin D Contents with Histological Type of Gastric Carcinoma

Abstract

The aim of this work was to evaluate the cytosolic contents of hyaluronic acid (HA) and cathepsin D (CatD) in gastric carcinomas and their possible relationships with the clinicopathological parameters of the tumors. Our study demonstrated a wide variability in the cytosolic levels of HA (mean +/- SEM: 3748 +/- 411 ng/mg protein) and cathepsin D (52 +/- 4 pmol/mg protein) in the tumors of 78 gastric cancer patients. In addition, the tumoral contents of HA and CatD were significantly higher (p<0.005) in diffuse type (HA: 6027 +/- 1099 ng/mg protein; CatD: 75 +/- 13 pmol/mg protein) than in intestinal type (HA: 2735 +/- 242 ng/mg protein; CatD: 42 +/- 3 pmol/mg protein) carcinomas. These data suggest that both markers may contribute to the biological characterization of gastric carcinomas.

Prognostic value of cytosolyc cathepsin D content in resectable gastric cancer

“…This variability of the protease content in adjacent nonneoplastic mucosa seems to correspond to the physiological status of the mucosa, whereas the variability in tumor protease content may be in relation to the biological heterogeneity of gastric carcinomas. The results of the present study also confirm our prior observation on a significant higher Cath-D content in carcinomas than in non-neoplastic gastric mucosa [18]. This latter finding suggests that the high Cath-D production in gastric carcinomas may be due to the processes associated with the malignant transformation of healthy gastric cells.…”

Significance of Elevated Levels of Collagen Type IV and Hyaluronic Acid in Gastric Juice and Serum in Gastric Cancer and Precancerous Lesion

“…Abnormal cytoplasmic staining pattern of cathepsin D reflects the invasive potential of gastric cancer cells. In diffuse-type gastric cancer, cathepsin D expression in cancer cells may play an important role in tumor progression, while in intestinal-type gastric cancer, cathepsin D expression in stromal cells may play an important role in tumor progression [30,31]. The incidence of lymph node metastasis is significantly related with the high expression [32].…”

Hyaluronic Acid Interacting Molecules Mediated Crosstalk between Cancer Cells and Microenvironment from Primary Tumour to Distant Metastasis

“…Poor differentiation, metastasis, and aggressive phenotype [27] Serum HA before chemotherapy Chemotherapy resistant; shortened OS and DFS [28,29] Endometrial cancer HA level Enhanced invasion, tumour grading and lymphatic involvement [30] Tumour stromal HA Tumour development [31] Peritumour stroma HA Tumour grade and invasion [32] Gastric cancer HA level Lymph node metastasis, cancer subtype specific, worse survival outcome. [33,34] Tumoral HA Cancer-subtype specific [35] Serum HA Elevated in gastric cancer [36] Lung cancer Baseline plasma HA Bone metastasis; chemotherapy efficacy [37] HA content Histological subtype specific, tumour differentiation, stage, recurrence and DFS [38] Tumour HA Level of malignancy, angiogenesis, patient survival, reflected in sputum [8] Mesothelioma HA level Increased in pleural fluid [39] High effusion HA level Better survival…”