Relative amounts and molecular forms of NESP55 in various bovine tissues

Lovisetti-Scamihorn, Paola; Fischer‐Colbrie, Reiner; Leitner, Bernd; Scherzer, Gerhard; Winkler, Hans

doi:10.1016/s0006-8993(99)01345-1

Cited by 38 publications

(25 citation statements)

References 35 publications

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“…The protein is highly conserved among mammals (80% amino acid identity between mouse and human) and shares features with the chromogranin family of proteins in its hydrophilicity, acidity, and association with secretory vesicles of neuroendocrine cells (3,18). Proteolytic processing occurs to different extents in various tissues and results in the release of the carboxy-terminal octapeptide (30). Recent results show that Nesp55 is associated with fast anterograde axonal transport in the peripheral nervous system and is considered a marker for the constitutive secretory pathway (13,27).…”

Section: Discussionmentioning

confidence: 99%

Imprinted Nesp55 Influences Behavioral Reactivity to Novel Environments

Plagge

Isles

Gordon

et al. 2005

Molecular and Cellular Biology

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134

112

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Genomic imprinting results in parent-of-origin-dependent monoallelic expression of selected genes. Although their importance in development and physiology is recognized, few imprinted genes have been investigated for their effects on brain function. Gnas is a complex imprinted locus whose gene products are involved in early postnatal adaptations and neuroendocrine functions. Gnas encodes the stimulatory G-protein subunit Gs␣ and two other imprinted protein-coding transcripts. Of these, the Nesp transcript, expressed exclusively from the maternal allele, codes for neuroendocrine secretory protein 55 (Nesp55), a chromogranin-like polypeptide associated with the constitutive secretory pathway but with an unknown function. Nesp is expressed in restricted brain nuclei, suggesting an involvement in specific behaviors. We have generated a knockout of Nesp55 in mice. Nesp55-deficient mice develop normally, excluding a role of this protein in the severe postnatal effects associated with imprinting of the Gnas cluster. Behavioral analysis of adult Nesp55 mutants revealed, in three separate tasks, abnormal reactivity to novel environments independent of general locomotor activity and anxiety. This phenotype may be related to prominent Nesp55 expression in the noradrenergic locus coeruleus. These results indicate a role of maternally expressed Nesp55 in controlling exploratory behavior and are the first demonstration that imprinted genes affect such a fundamental behavior.

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Section: Discussionmentioning

confidence: 99%

Imprinted Nesp55 Influences Behavioral Reactivity to Novel Environments

Plagge

Isles

Gordon

et al. 2005

Molecular and Cellular Biology

Self Cite

134

112

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show abstract

“…Disruption of alternative Gnas products could also play a role in dysregulation of energy metabolism. NESP55 is a maternal-specific Gnas gene product expressed primarily in the adrenal medulla and brain (16,17). However, NESP55 probably does not play a role in the m-/+ phenotype, as the exon 2 insertion does not disrupt the NESP55 coding region, and preliminary results suggest that adrenal expression of NESP55 mRNA is intact in m-/+ mice (S. Yu and L.S.…”

Section: Discussionmentioning

confidence: 99%

Paternal versus maternal transmission of a stimulatory G-protein α subunit knockout produces opposite effects on energy metabolism

Yu¹,

Гаврилова²,

Chen³

et al. 2000

J. Clin. Invest.

145

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“…The Nesp55 protein has similarities with the chromogranin family, is associated with secretory vesicles in neuroendocrine cells and is regarded as a marker for the constitutive secretory pathway (Fischer-Colbrie et al 2002). Little is known about its molecular function, but the protein is processed into peptides to variable extent in different cell types (Lovisetti-Scamihorn et al 1999). In agreement with its predominant expression in the nervous system and endocrine tissues (Bauer et al 1999a,b), mice deficient for Nesp55 show a behavioural phenotype, specifically an altered response to novel environments (Plagge et al 2005, Isles et al manuscript in preparation) but, in contrast to Ga s -and XLa s -deficient mice (see below), they exhibit no major effects on development, growth or metabolism.…”

Section: The Protein Coding Transcriptsmentioning

confidence: 99%

Physiological functions of the imprinted Gnas locus and its protein variants Gαs and XLαs in human and mouse

Plagge

Kelsey

Germain‐Lee

2007

Journal of Endocrinology

105

137

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The stimulatory a-subunit of trimeric G-proteins Ga s , which upon ligand binding to seven-transmembrane receptors activates adenylyl cyclases to produce the second messenger cAMP, constitutes one of the archetypal signal transduction molecules that have been studied in much detail. Over the past few years, however, genetic as well as biochemical approaches have led to a range of novel insights into the Ga s encoding guanine nucleotide binding protein, a-stimulating (Gnas) locus, its alternative protein products and its regulation by genomic imprinting, which leads to monoallelic, parental origin-dependent expression of the various transcripts. Here, we summarise the major characteristics of this complex gene locus and describe the physiological roles of Ga s and its 'extra large' variant XLa s at post-natal and adult stages as defined by genetic mutations. Opposite and potentially antagonistic functions of the two proteins in the regulation of energy homeostasis and metabolism have been identified in Gnasand Gnasxl (XLa s )-deficient mice, which are characterised by obesity and leanness respectively. A comparison of findings in mice with symptoms of the corresponding human genetic disease 'Albright's hereditary osteodystrophy'/'pseudohypoparathyroidism' indicates highly conserved functions as well as unresolved phenotypic differences.

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Relative amounts and molecular forms of NESP55 in various bovine tissues

Cited by 38 publications

References 35 publications

Imprinted Nesp55 Influences Behavioral Reactivity to Novel Environments

Imprinted Nesp55 Influences Behavioral Reactivity to Novel Environments

Paternal versus maternal transmission of a stimulatory G-protein α subunit knockout produces opposite effects on energy metabolism

Physiological functions of the imprinted Gnas locus and its protein variants Gαs and XLαs in human and mouse

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