“…The evaluation of progesterone levels in blood and milk, 20-25 days after insemination, has been used for the diagnosis of pregnancy (Robertson & Sarda, 1971; Gadsby, Heap, Henville & Laing, 1974; Pope, Majzlik, Ball & Leaver, 1976;Bulman & Lamming, 1976;Hoffmann, Günzler, Hamburger & Schmidt, 1976; Heap, Holdsworth, Gadsby, Laing & Walters, 1976;Pennington, Spahr & Lodge, 1976a;Holness, Ellison & Wilkins, 1977;Erb, Chew & Keller, 1977), but determinations in milk offer considerable advantages because milk is easier to collect than blood. Various methods have been described for the assay of progesterone in milk, either directly in small amounts of milk (Gadsby et al, 1974;Bulman & Lamming, 1976;Heap et ai, 1976;Dobson & Fitzpatrick, 1976;Allen, Redshaw & Holdsworth, 1980) or after extraction with petroleum ether (Schiavo, Matuszczak, Oltanacu & Foote, 1975;Pennington et ai, 1976a) or petroleum ether and methanol (Hoffmann et al, 1976), but the existence of non-specific interference, the high solubility of progesterone in fat and the effect of variations in fat content on the amount of progesterone determined in different milk samples (Pope et al, 1976) give low precision and accuracy of the assays (Schiavo et al, 1975;Heap et al, 1976).…”