RELATIVE CONTRIBUTION OF NF-κB AND AP-1 IN THE MODULATION BY CURCUMIN AND PYRROLIDINE DITHIOCARBAMATE OF THE UVB-INDUCED CYTOKINE EXPRESSION BY KERATINOCYTES

Hydrogen sulfide inhibits IL-8 expression in human keratinocytes via MAP kinase signaling

“…In line with previous data reporting that NCTC cells can secrete IL-8 under UV stimulation, 39 we found that these cells spontaneously secrete detectable levels of IL-8 in culture. Knowing that IL-17 and IL-22 act as inducers of IL-8 secretion and that ERK activation is required for IL-8 production, we preliminary tested the sensitivity of NCTC cells to IL-17 and IL-22, specifically focusing on the kinetics of ERK activation, the levels of IL-8 mRNA production and IL-8 protein secretion.…”

The toll-like receptor 3 pathway in homeostasis, responses to injury and wound repair

“…More specifically, UVB-damaged keratinocytes release U1 spliceosomal non-coding RNA that activates TLR3 in non-irradiated cells, resulting in the release of inflammatory cytokines, such as TNF-α and IL-6 [49]. These effects are consistent with findings from earlier studies, showing that UV-radiation activated NF-κB [50], triggered the pro-inflammatory MAPK p38 and c-Jun N-terminal kinase signalling arms [51][52][53], and promoted IL-6 and TNF-α release [54,55]. In light of subsequent studies on U1 RNA-mediated TLR3 activation, it seems likely that this pathway is involved in initiating these UVB-triggered inflammatory responses.…”

Curcumin inhibits the expression of COX-2 in UVB-irradiated human keratinocytes (HaCaT) by inhibiting activation of AP-1: p38 MAP kinase and JNK as potential upstream targets

“…COX-2 plays important roles in the development of carcinogenesis as well as inflammation in UVBirradiated skin (Grandjean-Laquerriere A et al, 2002;Surh YJ, 2003;Wilgus et al, 2003;Cui et al, 2004;Nijsten et al, 2004). Curcumin is known to exert its anti-inflammatory action in various cell lines through inhibition of COX-2 expression (Chun et al, 2003;Kang et al, 2004).…”