Relative efficacy of conventional sperm parameters and sperm penetration bioassay to assess bull fertility in vitro

Brahmkshtri, B. P.; Edwin, M. J.; John, M.C; Nainar, A. Mahalinga; Krishnan, Arunkumar

doi:10.1016/s0378-4320(98)00108-0

Cited by 44 publications

(19 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The ability to select high-fertility sires results in the production of semen samples with optimal quality that will ultimately improve conception rates. Currently, routine evaluation of sperm concentration and motility is frequently used to assess semen quality but provides limited information about the fertility indexes of sires [21][22][23][24][25][26]. Other criteria such as computerized analysis of motility and acrosome integrity also help to estimate semen quality and have been related to nonreturn rates of bulls, but correlations are not high or even consistent [27][28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

2015

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

2015

View full text Add to dashboard Cite

“…2000). Fertility was also evaluated in vitro using the so‐called oocyte penetration tests, with varying degrees of relation‐to‐sire fertility (Henault and Killian 1995; Brahmkshtri et al. 1999), and where interbull/ejaculate variation could be diminished by testing spermatozoa from several sires simultaneously (Henault and Killian 1995).…”

Section: Correlations Between ‘Functional’ Laboratory Assays and Fertmentioning

confidence: 99%

Laboratory Semen Assessment and Prediction of Fertility: still Utopia?*

Rodríguez‐Martínez

2003

Reprod Domestic Animals

255

156

View full text Add to dashboard Cite

Finding a laboratory test reliable enough to predict the potential fertility of a given semen sample or a given sire for artificial insemination (AI) is still considered utopian, as indicated by the modest correlations seen between results obtained in vitro and field fertility. Male fertility is complex, and depends upon a heterogeneous population of spermatozoa interacting at various levels of the female genital tract, the vestments of the oocyte, and the oocyte itself. For this reason, laboratory assessment of semen must include the testing of most sperm attributes relevant for fertilization and embryo development, not only in individual spermatozoa but within a large sperm population as well. Strategies for the discovery of in vitro predictors of semen fertility require evaluations of low sperm doses for AI, so that differences in innate in vivo fertility can be accurately detected.

show abstract

“…Thus, the hamster oocyte penetration test has been used successfully to assess the sperm fertilizing ability of the bull, the stallion and the red deer (Brackett et al. 1982; Brahmkshtri et al. 1999; Soler and Garde 2003).…”

Section: Discussionmentioning

confidence: 99%

Heterologous In Vitro Fertility Evaluation of Cryopreserved Iberian Red Deer Epididymal Spermatozoa with Zona‐intact Sheep Oocytes and its Relationship with the Characteristics of Thawed Spermatozoa

Soler

Poulin

Fernandez-Santos

et al. 2007

Reprod Domestic Animals

View full text Add to dashboard Cite

A heterologous in vitro system, using zona-intact sheep oocytes, was used to evaluate the relationship between sperm factors of Iberian red deer thawed epididymal sperm and the percentage of cleaved oocytes. Epididymal spermatozoa were recovered from six males, diluted with freezing extender and cryopreserved. After thawing sperm motility (SM) and acrosome and membrane integrities were evaluated. Again, these parameters were assessed after incubation in freezing extender at 37 degrees C for 2 h. After cryopreservation the values for SM and acrosome and membrane integrities were high (approximately 80, 80 and 70% respectively). However, these values significantly decreased after incubation (approximately 59, 62 and 47% respectively). Red deer thawed epididymal sperm fertilized zona-intact sheep oocytes, although the percentage of cleaved oocytes was low (approximately 22%). No relationship was found between sperm parameters assessed after thawing and the percentage of cleaved oocytes. Likewise, any sperm parameter evaluated after incubation was assessed in relation to the percentage of cleaved oocytes. However, acrosome and membrane integrities were near to significance (p = 0.06 and p = 0.09 respectively). Then, we conducted a reduced model with these two variables and both were related to the percentage of cleaved oocytes (p = 0.02 and p = 0.04 respectively). Thus, acrosome and membrane integrities were related to the percentage of cleaved oocytes negatively and positively respectively. It was concluded that the classical parameters assessed in deer thawed sperm samples can be good predictors of the ability to fertilize zona-intact sheep oocytes.

show abstract

Relative efficacy of conventional sperm parameters and sperm penetration bioassay to assess bull fertility in vitro

Cited by 44 publications

References 17 publications

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

Seminal plasma and serum fertility biomarkers in dromedary camels (Camelus dromedarius)

Laboratory Semen Assessment and Prediction of Fertility: still Utopia?*

Heterologous In Vitro Fertility Evaluation of Cryopreserved Iberian Red Deer Epididymal Spermatozoa with Zona‐intact Sheep Oocytes and its Relationship with the Characteristics of Thawed Spermatozoa

Contact Info

Product

Resources

About