1994
DOI: 10.1159/000462501
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Relative Efficiency of Leucocyte Removal Procedures for the Production of Leucocyte-Poor Red Cell Concentrates Assessed by Flow Cytometry

Abstract: Flow cytometry was used to: (1) determine residual leucocyte numbers in red cell suspensions following the range of leucocyte depletion procedures used in our organisation, and (2) to characterise phenotypically the leucocytes using direct immuofluorescence with monoclonal antibodies to cell surface receptors. Under the conditions used, a lower limit of detection of 2.5 leucocytes per µl (equivalent to 3.43 log(10) or 99.96% removal) could be achieved. Filtration through polyester filters was found to remove u… Show more

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Cited by 4 publications
(5 citation statements)
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“…After freezing and deglycerolization, residual WBCs are essentially lymphocytes, confirming previous reports 11,19 . All of the WBCs are not enclosed in the respective gates set for fresh cells as indicated by the phenotype analysis, but WBCs are associated with adherent fragments and express more than one phenotype.…”
Section: Discussionsupporting
confidence: 82%
See 1 more Smart Citation
“…After freezing and deglycerolization, residual WBCs are essentially lymphocytes, confirming previous reports 11,19 . All of the WBCs are not enclosed in the respective gates set for fresh cells as indicated by the phenotype analysis, but WBCs are associated with adherent fragments and express more than one phenotype.…”
Section: Discussionsupporting
confidence: 82%
“…WBC reduction using commercially available filters can surpass the US standard, producing a WBC reduction greater than 3 logs, 9,16‐18 and WBC reduction of transfused blood components has become increasingly common, although not universally applied in all centers 10 . The use of WBC filters before RBC cryopreservation results in the loss of approximately 20 mL of blood volume, adds supplemental expenses, and requires additional time and manipulation 16,19 . Therefore, we examined alternative methods for WBC reduction and the goal of this study was to evaluate the extent of WBC reduction achieved in frozen/thawed RBC units with and without prior buffy‐coat (BC) removal.…”
mentioning
confidence: 99%
“…Farrugiaetal. [12] discussed an FC method similar to that of Wenz et al [9]. Like us, they used the LeucoGATE combi nation of MAB to show reduction of granulocytes by fil tration of blood through polyester filters made by different producers and by additional washing of RBC after filtration or after an additional filtration.…”
Section: Discussionmentioning
confidence: 99%
“…In the Edinburgh Regional Transfusion Centre of the Scottish National Blood Transfusion Service (SNBTS) we began to look at automated leucocyte counting methods for assessment of the effectiveness of leucodepletion in late 1997, in anticipation of provision in 1998 of leucodepleted blood for neonatal use. Since a Becton-Dickinson flow cytometer was available, we opted to evaluate flow cytometry technology for leucocyte counting following a number of reports of its suitability (Bodensteiner, 1989;Dzik et al, 1990;Wenz et al, 1991;Sheckler & Loken, 1993;Vachula et al, 1993;Farrugia et al, 1994;Rebulla & Dzik, 1994;Dumont & Dumont, 1996).…”
mentioning
confidence: 99%