Relative efficiency of the maize‐ and <i>Imperata cylindrica</i>‐mediated chromosome elimination approaches for induction of haploids of wheat‐rye derivatives

Kishore, Naval; Chaudhary, H. K.; Chahota, Rakesh Kumar; Kumar, Vijay; Sood, Salej; Muniyandi, Samuel Jeberson; Tayeng, Tisu

doi:10.1111/j.1439-0523.2010.01793.x

Cited by 31 publications

(23 citation statements)

References 15 publications

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“…The spikes were harvested 14 days after pollination, and the seeds were examined for the presence of embryos. Embryos were found and cultured on an MS nutrient medium (Murashige and Skoog, 1962) The glass culture tubes with embryos were incubated at 4 °C overnight to activate the stress before transfer to 20 ± 2 °C in a 10-h light/14-h dark photoperiod regime until regeneration (Kishore et al, 2011). The regenerated plantlets were then transferred to a liquid MS medium with the same ingredients as the nutrient medium to improve the root growth.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, it is available under natural conditions wherever wheat is cultivated. Compared with other chromosome elimination techniques, including maize hybridization, this technique is much more efficient, practical, and economical Kishore et al, 2011). On the other hand, obtaining higher percentages of haploid regenerants from all the studied species proved that this technique, unlike the others, is not genotypespecific .…”

Section: Introductionmentioning

confidence: 92%

“…On the other hand, obtaining higher percentages of haploid regenerants from all the studied species proved that this technique, unlike the others, is not genotypespecific . Imperata cylindrica L. mediated chromosome elimination has been applied to date only on the species Triticum aestivum L. Pratap et al, 2005;Komeda et al, 2007;Tayeng et al, 2012) and Triticum aestivum L. × Secale cereale L. hybrids (Kishore et al, 2011). Therefore, the objective of the present study was to evaluate the possibility of obtaining dihaploid durum wheat (Triticum durum L.) plants by interspecific hybridization with Imperata cylindrica L.…”

Section: Introductionmentioning

confidence: 99%

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Production of dihaploids in durum wheat using Imperata cylindrica L. mediated chromosome elimination

Çeli̇ktaş¹,

Tiryakioğlu²,

Can³

et al. 2015

Turk J Agric For

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The possibility of obtaining dihaploid durum wheat (Triticum durum L.) plants by interspecific hybridization with Imperata cylindrica L. was studied. Ten local varieties and 1 commercial durum wheat variety (Triticum durum L., 2n = 4x = 28; AABB) were used as maternal lines. Imperata cylindrica L. (2n = 2x = 20) ecotypes sampled from different districts of Hatay were used as the pollen source. Isolated embryos were placed in an MS medium for plant regeneration 14 days after the interspecific hybridization. The highest average seed setting, embryo formation, and dihaploid plant regeneration for the different hybrid combinations were determined as 31.1%, 15.5%, and 11.5%, respectively. Cytological observations revealed that all the regenerants had 2n = 2x = 14 chromosomes.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Production of dihaploids in durum wheat using Imperata cylindrica L. mediated chromosome elimination

Çeli̇ktaş¹,

Tiryakioğlu²,

Can³

et al. 2015

Turk J Agric For

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“…Recently wheat × Imperata cylindrica (Chaudhary et al 2005) system has emerged as a highly efficient alternative for revolutionizing the doubled haploid production scenario. This system has responded to haploid induction significantly better than wheat × Z. mays system, not only in wheat × wheat hybrids (Chaudhary et al 2005;2013a and2013b;Chaudhary 2008a;2008b;2009;2010a;2010b;2012;2013a and2013b;Kaila et al 2012;Rather 2012;Tayeng et al 2012) but also been explored in triticale × wheat Pratap and Chaudhary 2007;Badiyal et al 2014) and wheat × rye derivatives (Kishore et al 2011) efficiently, where wheat × maize system failed altogether.…”

Section: Introductionmentioning

confidence: 99%

Comparative pollination efficiency of freshly harvested pollen ofImperata cylindricaandZea maysfor haploid induction in bread wheat

Mayel

Chaudhary

Badiyal

et al. 2016

Cereal Research Communications

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Pollen viability is among the critical factors, which determine the success of a doubled haploidy breeding programme, thus the present investigation was undertaken to assess the functional viability and relative longevity of the pollen from Zea mays and Imperata cylindrica for the chromosome elimination mediated approach of doubled haploidy breeding. Two wheat genotypes representing spring and winter ecotypes, namely DH 40 and Saptdhara were pollinated with freshly harvested pollen of two known potential haploid inducing sources, namely Z. mays (grown in polyhouse conditions) and I. cylindrica (wild grass) for 15 and 17 days, respectively, keeping five minutes interval between two subsequent pollinations. The results revealed a significant decrease in the viability of Z. mays pollen up to 95 percent in Saptdhara and 85 percent in DH 40 within one hour, whereas Imperata pollen was found to be functionally viable even at the end of experimentation period, showing high embryo formation in both the wheat parents. I. cylindrica exhibited higher frequencies for haploid induction parameters in both the wheat parents as compared to Z. mays. I. cylindrica pollen, being viable for relatively longer periods than Z. mays can hasten the haploid induction endeavours, thus may be a more efficient alternative to Z. mays for breeding programmes using doubled haploidy technique.

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“…anther culture (Barnabas 2003) and chromosome elimination approaches like wheat × Hordeum bulbosum (Barclay 1975), wheat × maize (Laurie & Bennett 1986) and wheat × Imperata cylindrica system , have emerged and are being utilized in different wheat improvement programmes. Among all these available systems of doubled haploid production in wheat, the I. cylindrica-mediated approach has shown its worth in terms of haploid embryo induction frequency not doi: 10.17221/218/2014-CJGPB only in bread wheat but also in triticale × wheat and wheat × rye derivatives Kishore et al 2011). The underlined mechanism behind all the intergeneric hybridization-mediated systems is the elimination of paternal chromosomes during the initial zygotic cell divisions (Komeda et al 2007;Chaudhary et al 2013).…”

mentioning

confidence: 99%

Dihaploid induction in tetraploid durum wheat (Triticum durum L.) using pollen of Imperata cylindrica

Chaudhary¹,

Mahato²,

Kaila³

et al. 2015

CZECH J GENET PLANT

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Chaudhary H.K., Mahato A., Kaila V., Rather S.A., Tayeng T. (2015): Dihaploid induction in tetraploid durum wheat (Triticum durum L.) using pollen of Imperata cylindrica. Czech J. Genet. Plant Breed., 51: 142-147. Doubled haploidy breeding protocols have revolutionized the varietal development programmes in bread wheat, however, the protocols have not proved much efficient in durum wheat. Presently, the durum wheat × maize system is being widely followed for haploid induction but the frequency of haploid development is very poor which has limited its application in durum wheat improvement programmes. In order to formulate an efficient wide hybridization-mediated approach for haploid induction in durum wheat, different genotypes of durum wheat were subjected to hybridization with Imperata cylindrica, a wild perennial grass for the first time in this laboratory. The investigation was carried out for two seasons. During the 1 st year, the investigation was carried out on one genotype only, Langdon, in order to notice the development of haploid embryos and the factors that influence the haploid induction frequency. The most important factor influencing the embryo formation frequency was found to be the concentration of 2,4-dichlorophenoxyacetic acid (2,4-D). Upon pollinating the emasculated spikes of durum wheat genotypes, various concentrations of 2,4-D were injected into the uppermost internode of wheat culm for three consecutive days after pollination to find out the most responding concentration for haploid induction. During the next year, the protocol was applied to eight durum wheat genotypes. The frequency of haploid induction parameters varied with the durum wheat genotypes as well as 2,4-D concentration used. The mean pseudoseed and embryo formation frequency over all the genotypes ranged from 30.2 to 56.3% and 1.2 to 18.4%, respectively. The average embryo formation frequency over all the genotypes was found to be highest (18.4%) at 250 mg/l 2,4-D whereas it was superior for the genotypes WH 896 and Langdon over all the 2,4-D concentrations. At the most responding 2,4-D concentration (250 mg/l), the genotype A-9-30-1 exhibited the highest embryo formation frequency (32.1%). The ploidy status of the developed embryos was identified using cytological analysis carried out on the rootlets of the tissue culture generated plantlets.

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Relative efficiency of the maize‐ and Imperata cylindrica‐mediated chromosome elimination approaches for induction of haploids of wheat‐rye derivatives

Cited by 31 publications

References 15 publications

Production of dihaploids in durum wheat using Imperata cylindrica L. mediated chromosome elimination

Production of dihaploids in durum wheat using Imperata cylindrica L. mediated chromosome elimination

Comparative pollination efficiency of freshly harvested pollen ofImperata cylindricaandZea maysfor haploid induction in bread wheat

Dihaploid induction in tetraploid durum wheat (Triticum durum L.) using pollen of Imperata cylindrica

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